AFAP1 ORF clone FHC11642

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11642

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Clone info.

Species

human

Clone Name

pFN21AE1022 Sequence (with vector) Restriction Map

Product ID

FHC11642

Flexi Clone PID

FXC11642

Original Clone PID

ORK11642

ORF Sequence

DNA sequence (2190 bp)
Protein sequence (730 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527433]

Gene

KIEE1022

Symbol

AFAP1

Alias : AFAP, AFAP-110, AFAP110

Description

actin filament associated protein 1, transcript variant 2

Original Clone ID

ef06191

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2190 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 730 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3397.1 4.6e-175 99.9 AFAP1 CCDS47010.1 7.4e-123 88.8 AFAP1 CCDS34274.1 4e-49 46.9 AFAP1L1 CCDS54932.1 4.1e-49 48.4 AFAP1L1 CCDS31287.1 2.4e-19 36.3 AFAP1L2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 159 247 PF00169 Pleckstrin homology domain IPR001849 351 434 PF00169 Pleckstrin homology domain HMMSmart IPR001849 154 251 SM00233 Pleckstrin homology domain IPR001849 348 443 SM00233 Pleckstrin homology domain ProfileScan IPR001849 153 249 PS50003 Pleckstrin homology domain IPR001849 347 441 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_940997 730 608252 99.9 100.0 XP_006713972 751 608252 99.9 100.0 XP_016864024 748 608252 97.5 100.0 XP_011511846 814 608252 88.8 92.9 NP_001128119 814 608252 88.8 92.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1022 Download

>KIEE1022  2190 bp
ATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCTTGAACTCCTGGACCATGAATATCTA
ACCTCAACTGTCAGGGAGAAAAAGGCAGTGATAACCAACATTCTGCTAAGAATACAGTCA
TCCAAAGGTTTTGATGTGAAGGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCTGCCA
GCCCCTCCTCAGATGCCCCTGCCGGAGATCCCTCAGCCCTGGCTGCCTCCTGACAGTGGG
CCTCCACCATTGCCAACATCCTCCCTCCCAGAAGGTTATTATGAGGAAGCTGTGCCGCTG
AGCCCCGGAAAAGCTCCGGAATACATCACATCAAATTATGATTCCGATGCGATGAGCAGC
TCTTATGAGTCGTATGATGAAGAGGAGGAGGATGGGAAGGGGAAGAAAACCCGGCACCAG
TGGCCCTCCGAGGAGGCCTCCATGGACCTGGTCAAGGACGCCAAAATCTGCGCCTTCCTG
CTGCGGAAGAAGCGGTTCGGCCAGTGGACCAAGTTGCTCTGCGTCATCAAAGACACCAAA
CTGCTGTGCTATAAAAGTTCCAAGGACCAGCAGCCTCAGATGGAACTGCCACTCCAAGGC
TGTAACATTACGTACATCCCGAAAGACAGCAAAAAGAAGAAGCACGAGCTGAAGATTACT
CAGCAGGGCACGGACCCGCTTGTTCTCGCCGTCCAGAGCAAGGAACAGGCCGAGCAGTGG
CTGAAGGTGATCAAAGAAGCCTACAGTGGTTGTAGTGGCCCCGTGGATTCAGAGTGTCCT
CCTCCACCAAGCTCCCCGGTGCACAAGGCAGAACTGGAGAAGAAACTGTCTTCAGAGAGA
CCCAGCTCAGATGGGGAGGGTGTTGTGGAAAATGGAATTACCACATGTAATGGAAAGGAG
CAAGTGAAGAGGAAGAAAAGTTCCAAATCAGAGGCCAAGGGCACTGTGTCGAAAGTCACT
GGGAAAAAAATCACCAAGATCATCAGTCTGGGAAAGAAAAAGCCGTCCACAGACGAGCAG
ACCTCCTCAGCTGAGGAAGATGTTCCCACCTGCGGCTATCTGAACGTGCTCTCCAACAGC
CGCTGGCGAGAGCGCTGGTGCCGAGTGAAAGATAACAAGCTCATTTTCCACAAGGACAGG
ACTGACCTGAAGACCCATATTGTGTCTATTCCGCTCCGTGGCTGCGAGGTGATCCCGGGT
TTGGATTCTAAACATCCTCTGACGTTCCGGCTGCTGCGCAACGGCCAGGAGGTTGCAGTA
TTGGAGGCATCTTCTTCTGAAGACATGGGCAGGTGGATTGGGATTTTACTCGCAGAGACG
GGATCGTCCACAGACCCGGAGGCTCTGCACTATGACTACATTGATGTGGAGATGTCTGCA
AGTGTCATTCAGACAGCCAAACAGACCTTCTGTTTCATGAACAGGCGTGTTATATCTGCT
AACCCATATCTAGGGGGCACCTCCAACGGCTATGCCCACCCCAGCGGGACGGCACTTCAT
TATGACGATGTCCCGTGCATCAACGGCTCGCTCAAGGGTAAAAAGCCCCCCATGGCGTCT
AATGGGGTCACAGGAAAAGGGAAGACTCTGAGCAGTCAGCCAAAGAAAGCGGATCCCGCG
GCTGTTGTGAAAAGGACGGGTTCGAATGCTGCCCAGTACAAGTATGGCAAGAACCGGGTT
GAAGCAGATGCCAAGCGGCTACAGACCAAAGAGGAGGAGCTGCTGAAGAGGAAAGAGGCC
CTGCGGAATAGGCTGGCCCAGCTCCGCAAGGAAAGAAAAGACCTTCGAGCGGCTATTGAA
GTGAACGCCGGCAGGAAGCCGCAGGCGATCCTGGAGGAGAAGCTGAAGCAGCTGGAGGAG
GAGTGCCGGCAGAAGGAGGCGGAGCGTGTCAGCCTGGAGCTGGAGCTGACGGAGGTCAAG
GAGAGCCTGAAGAAAGCGCTGGCGGGCGGAGTCACCCTGGGGCTGGCCATCGAGCCCAAG
TCAGGGACATCGAGTCCACAGTCTCCAGTGTTCAGGCACCGGACCCTGGAAAACTCGCCC
ATCTCCAGCTGTGACACCAGTGACACCGAGGGCCCCGTGCCGGTGAACAGCGCGGCCGTC
TTGAAGAAGAGCCAGGCTGCCCCGGGCAGCTCCCCCTGCCGAGGGCATGTGCTGCGGAAG
GCCAAGGAATGGGAATTGAAGAACGGGACC

ORF Protein

Cloned ORF protein sequence for pFN21AE1022 Download

>KIEE1022  730 aa
MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLP
APPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSS
SYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTK
LLCYKSSKDQQPQMELPLQGCNITYIPKDSKKKKHELKITQQGTDPLVLAVQSKEQAEQW
LKVIKEAYSGCSGPVDSECPPPPSSPVHKAELEKKLSSERPSSDGEGVVENGITTCNGKE
QVKRKKSSKSEAKGTVSKVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNS
RWRERWCRVKDNKLIFHKDRTDLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAV
LEASSSEDMGRWIGILLAETGSSTDPEALHYDYIDVEMSASVIQTAKQTFCFMNRRVISA
NPYLGGTSNGYAHPSGTALHYDDVPCINGSLKGKKPPMASNGVTGKGKTLSSQPKKADPA
AVVKRTGSNAAQYKYGKNRVEADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAAIE
VNAGRKPQAILEEKLKQLEEECRQKEAERVSLELELTEVKESLKKALAGGVTLGLAIEPK
SGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAVLKKSQAAPGSSPCRGHVLRK
AKEWELKNGT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1022 Download

>pFN21AE1022  6895 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCT
TGAACTCCTGGACCATGAATATCTAACCTCAACTGTCAGGGAGAAAAAGGCAGTGATAAC
CAACATTCTGCTAAGAATACAGTCATCCAAAGGTTTTGATGTGAAGGACCATGCTCAGAA
GCAGGAGACCGCTAACAGCCTGCCAGCCCCTCCTCAGATGCCCCTGCCGGAGATCCCTCA
GCCCTGGCTGCCTCCTGACAGTGGGCCTCCACCATTGCCAACATCCTCCCTCCCAGAAGG
TTATTATGAGGAAGCTGTGCCGCTGAGCCCCGGAAAAGCTCCGGAATACATCACATCAAA
TTATGATTCCGATGCGATGAGCAGCTCTTATGAGTCGTATGATGAAGAGGAGGAGGATGG
GAAGGGGAAGAAAACCCGGCACCAGTGGCCCTCCGAGGAGGCCTCCATGGACCTGGTCAA
GGACGCCAAAATCTGCGCCTTCCTGCTGCGGAAGAAGCGGTTCGGCCAGTGGACCAAGTT
GCTCTGCGTCATCAAAGACACCAAACTGCTGTGCTATAAAAGTTCCAAGGACCAGCAGCC
TCAGATGGAACTGCCACTCCAAGGCTGTAACATTACGTACATCCCGAAAGACAGCAAAAA
GAAGAAGCACGAGCTGAAGATTACTCAGCAGGGCACGGACCCGCTTGTTCTCGCCGTCCA
GAGCAAGGAACAGGCCGAGCAGTGGCTGAAGGTGATCAAAGAAGCCTACAGTGGTTGTAG
TGGCCCCGTGGATTCAGAGTGTCCTCCTCCACCAAGCTCCCCGGTGCACAAGGCAGAACT
GGAGAAGAAACTGTCTTCAGAGAGACCCAGCTCAGATGGGGAGGGTGTTGTGGAAAATGG
AATTACCACATGTAATGGAAAGGAGCAAGTGAAGAGGAAGAAAAGTTCCAAATCAGAGGC
CAAGGGCACTGTGTCGAAAGTCACTGGGAAAAAAATCACCAAGATCATCAGTCTGGGAAA
GAAAAAGCCGTCCACAGACGAGCAGACCTCCTCAGCTGAGGAAGATGTTCCCACCTGCGG
CTATCTGAACGTGCTCTCCAACAGCCGCTGGCGAGAGCGCTGGTGCCGAGTGAAAGATAA
CAAGCTCATTTTCCACAAGGACAGGACTGACCTGAAGACCCATATTGTGTCTATTCCGCT
CCGTGGCTGCGAGGTGATCCCGGGTTTGGATTCTAAACATCCTCTGACGTTCCGGCTGCT
GCGCAACGGCCAGGAGGTTGCAGTATTGGAGGCATCTTCTTCTGAAGACATGGGCAGGTG
GATTGGGATTTTACTCGCAGAGACGGGATCGTCCACAGACCCGGAGGCTCTGCACTATGA
CTACATTGATGTGGAGATGTCTGCAAGTGTCATTCAGACAGCCAAACAGACCTTCTGTTT
CATGAACAGGCGTGTTATATCTGCTAACCCATATCTAGGGGGCACCTCCAACGGCTATGC
CCACCCCAGCGGGACGGCACTTCATTATGACGATGTCCCGTGCATCAACGGCTCGCTCAA
GGGTAAAAAGCCCCCCATGGCGTCTAATGGGGTCACAGGAAAAGGGAAGACTCTGAGCAG
TCAGCCAAAGAAAGCGGATCCCGCGGCTGTTGTGAAAAGGACGGGTTCGAATGCTGCCCA
GTACAAGTATGGCAAGAACCGGGTTGAAGCAGATGCCAAGCGGCTACAGACCAAAGAGGA
GGAGCTGCTGAAGAGGAAAGAGGCCCTGCGGAATAGGCTGGCCCAGCTCCGCAAGGAAAG
AAAAGACCTTCGAGCGGCTATTGAAGTGAACGCCGGCAGGAAGCCGCAGGCGATCCTGGA
GGAGAAGCTGAAGCAGCTGGAGGAGGAGTGCCGGCAGAAGGAGGCGGAGCGTGTCAGCCT
GGAGCTGGAGCTGACGGAGGTCAAGGAGAGCCTGAAGAAAGCGCTGGCGGGCGGAGTCAC
CCTGGGGCTGGCCATCGAGCCCAAGTCAGGGACATCGAGTCCACAGTCTCCAGTGTTCAG
GCACCGGACCCTGGAAAACTCGCCCATCTCCAGCTGTGACACCAGTGACACCGAGGGCCC
CGTGCCGGTGAACAGCGCGGCCGTCTTGAAGAAGAGCCAGGCTGCCCCGGGCAGCTCCCC
CTGCCGAGGGCATGTGCTGCGGAAGGCCAAGGAATGGGAATTGAAGAACGGGACCGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT