ST8SIA4 ORF clone FHC11528

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Halotag ORF Clone FHC11528

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Clone info.

Species

human

Clone Name

pFN21AB4480 Sequence (with vector) Restriction Map

Product ID

FHC11528

Flexi Clone PID

FXC11528

ORF Sequence

DNA sequence (1077 bp)
Protein sequence (359 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529101]

Gene

KIBB4480

Symbol

ST8SIA4

Alias : PST, PST1, SIAT8D, ST8SIA-IV

Description

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4, transcript variant 1

Original Clone ID

ah03089

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1077 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 359 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4091.1 5.6e-165 100.0 ST8SIA4 CCDS81919.1 5.9e-97 58.3 ST8SIA2 CCDS10372.1 2e-94 57.9 ST8SIA2 CCDS47252.1 1e-71 100.0 ST8SIA4 CCDS32834.1 1.3e-48 39.3 ST8SIA3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001675 92 355 PF00777 Glycosyl transferase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005659 359 602547 100.0 100.0 NP_001317345 354 602546 58.3 97.8 NP_006002 375 602546 57.9 97.8 XP_016878131 396 602546 64.0 85.5 XP_011541932 261 602547 72.4 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4480 Download

>KIBB4480  1077 bp
ATGCGCTCCATTAGGAAGAGGTGGACGATCTGCACAATAAGTCTGCTCCTGATCTTTTAT
AAGACAAAAGAAATAGCAAGAACTGAGGAGCACCAGGAGACGCAACTCATCGGAGATGGT
GAATTGTCTTTGAGTCGGTCACTTGTCAATAGCTCTGATAAAATCATTCGAAAGGCTGGC
TCTTCAATCTTCCAGCACAATGTAGAAGGTTGGAAAATCAATTCCTCTTTGGTCCTAGAG
ATAAGGAAGAACATACTTCGTTTCTTAGATGCAGAACGAGATGTGTCAGTGGTCAAGAGC
AGTTTTAAGCCTGGTGATGTCATACACTATGTGCTTGACAGGCGCCGGACACTAAACATT
TCTCATGATCTACATAGCCTCCTACCTGAAGTTTCACCAATGAAGAATCGCAGGTTTAAG
ACCTGTGCAGTTGTTGGAAATTCTGGCATTCTGTTAGACAGTGAATGTGGAAAGGAGATT
GACAGTCACAATTTTGTAATAAGGTGTAATCTAGCTCCTGTGGTGGAGTTTGCTGCAGAT
GTGGGAACTAAATCAGATTTTATTACCATGAATCCATCAGTTGTACAAAGAGCATTTGGA
GGCTTTCGAAATGAGAGTGACAGAGAAAAATTTGTGCATAGACTTTCCATGCTGAATGAC
AGTGTCCTTTGGATTCCTGCTTTCATGGTCAAAGGAGGAGAGAAGCACGTGGAGTGGGTT
AATGCATTAATCCTTAAGAATAAACTGAAAGTGCGAACTGCCTATCCGTCATTGAGACTT
ATTCATGCTGTCAGAGGTTACTGGCTGACCAACAAAGTTCCTATCAAAAGACCCAGCACA
GGTCTTCTCATGTATACACTTGCCACAAGATTCTGTGATGAAATTCACCTGTATGGATTC
TGGCCCTTCCCTAAGGATTTAAATGGAAAAGCGGTCAAATATCATTATTATGATGACTTA
AAATATAGGTACTTTTCCAATGCAAGCCCTCACAGAATGCCATTAGAATTCAAAACATTA
AATGTGCTACATAATAGAGGAGCTCTAAAACTGACAACAGGAAAGTGTGTAAAGCAA

ORF Protein

Cloned ORF protein sequence for pFN21AB4480 Download

>KIBB4480  359 aa
MRSIRKRWTICTISLLLIFYKTKEIARTEEHQETQLIGDGELSLSRSLVNSSDKIIRKAG
SSIFQHNVEGWKINSSLVLEIRKNILRFLDAERDVSVVKSSFKPGDVIHYVLDRRRTLNI
SHDLHSLLPEVSPMKNRRFKTCAVVGNSGILLDSECGKEIDSHNFVIRCNLAPVVEFAAD
VGTKSDFITMNPSVVQRAFGGFRNESDREKFVHRLSMLNDSVLWIPAFMVKGGEKHVEWV
NALILKNKLKVRTAYPSLRLIHAVRGYWLTNKVPIKRPSTGLLMYTLATRFCDEIHLYGF
WPFPKDLNGKAVKYHYYDDLKYRYFSNASPHRMPLEFKTLNVLHNRGALKLTTGKCVKQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4480 Download

>pFN21AB4480  5782 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCGCTCCATTAGGAAGAGGTGGACGATCTGCAC
AATAAGTCTGCTCCTGATCTTTTATAAGACAAAAGAAATAGCAAGAACTGAGGAGCACCA
GGAGACGCAACTCATCGGAGATGGTGAATTGTCTTTGAGTCGGTCACTTGTCAATAGCTC
TGATAAAATCATTCGAAAGGCTGGCTCTTCAATCTTCCAGCACAATGTAGAAGGTTGGAA
AATCAATTCCTCTTTGGTCCTAGAGATAAGGAAGAACATACTTCGTTTCTTAGATGCAGA
ACGAGATGTGTCAGTGGTCAAGAGCAGTTTTAAGCCTGGTGATGTCATACACTATGTGCT
TGACAGGCGCCGGACACTAAACATTTCTCATGATCTACATAGCCTCCTACCTGAAGTTTC
ACCAATGAAGAATCGCAGGTTTAAGACCTGTGCAGTTGTTGGAAATTCTGGCATTCTGTT
AGACAGTGAATGTGGAAAGGAGATTGACAGTCACAATTTTGTAATAAGGTGTAATCTAGC
TCCTGTGGTGGAGTTTGCTGCAGATGTGGGAACTAAATCAGATTTTATTACCATGAATCC
ATCAGTTGTACAAAGAGCATTTGGAGGCTTTCGAAATGAGAGTGACAGAGAAAAATTTGT
GCATAGACTTTCCATGCTGAATGACAGTGTCCTTTGGATTCCTGCTTTCATGGTCAAAGG
AGGAGAGAAGCACGTGGAGTGGGTTAATGCATTAATCCTTAAGAATAAACTGAAAGTGCG
AACTGCCTATCCGTCATTGAGACTTATTCATGCTGTCAGAGGTTACTGGCTGACCAACAA
AGTTCCTATCAAAAGACCCAGCACAGGTCTTCTCATGTATACACTTGCCACAAGATTCTG
TGATGAAATTCACCTGTATGGATTCTGGCCCTTCCCTAAGGATTTAAATGGAAAAGCGGT
CAAATATCATTATTATGATGACTTAAAATATAGGTACTTTTCCAATGCAAGCCCTCACAG
AATGCCATTAGAATTCAAAACATTAAATGTGCTACATAATAGAGGAGCTCTAAAACTGAC
AACAGGAAAGTGTGTAAAGCAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT