SKAP2 ORF clone FHC11377

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11377

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Clone info.

Species

human

Clone Name

pFN21AE0604 Sequence (with vector) Restriction Map

Product ID

FHC11377

Flexi Clone PID

FXC11377

ORF Sequence

DNA sequence (1077 bp)
Protein sequence (359 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528968]

Gene

KIEE0604

Symbol

SKAP2

Alias : PRAP, RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R

Description

src kinase associated phosphoprotein 2, transcript variant 1

Original Clone ID

cs02104

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1077 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 359 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5400.1 5.9e-102 100.0 SKAP2 CCDS32674.1 3.3e-38 44.6 SKAP1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001452 314 329 PR00452 Src homology-3 domain IPR001452 333 342 PR00452 Src homology-3 domain IPR001452 344 356 PR00452 Src homology-3 domain HMMPfam IPR001849 118 217 PF00169 Pleckstrin homology domain IPR001452 313 349 PF00018 Src homology-3 domain HMMSmart IPR001849 117 221 SM00233 Pleckstrin homology domain IPR001452 300 357 SM00326 Src homology-3 domain ProfileScan IPR001849 116 219 PS50003 Pleckstrin homology domain IPR001452 297 358 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003921 359 605215 100.0 100.0 XP_016868260 359 605215 100.0 100.0 NP_003717 359 604969 44.6 96.7 NP_001068567 358 604969 44.3 96.7 XP_016880746 335 604969 43.6 84.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0604 Download

>KIEE0604  1077 bp
ATGCCCAACCCCAGCAGCACCTCCTCTCCCTACCCCCTCCCTGAGGAAATTAGGAACCTG
TTGGCAGATGTTGAAACATTTGTAGCAGATATACTGAAAGGAGAAAATTTATCCAAGAAA
GCAAAGGAAAAGAGAGAATCCCTTATTAAGAAGATAAAAGATGTAAAGTCTATCTATCTT
CAGGAATTTCAAGACAAAGGTGATGCAGAAGATGGGGAAGAATATGATGACCCTTTTGCT
GGGCCTCCAGACACTATTTCATTAGCCTCAGAACGATATGATAAAGACGATGAAGCCCCC
TCTGATGGAGCCCAGTTTCCTCCAATTGCAGCACAAGACCTTCCTTTTGTTCTAAAGGCT
GGCTACCTTGAAAAACGCAGAAAAGATCACAGCTTTCTGGGATTTGAATGGCAGAAACGG
TGGTGTGCTCTCAGTAAAACGGTATTCTATTATTATGGAAGTGATAAAGACAAACAACAG
AAAGGTGAATTTGCAATAGATGGCTACAGTGTCAGAATGAATAACACTCTAAGAAAGGAT
GGAAAGAAAGATTGCTGTTTTGAAATCTCTGCTCCTGATAAACGTATATATCAGTTTACA
GCAGCTTCTCCCAAAGATGCTGAAGAATGGGTACAGCAGCTGAAATTTGTATTGCAAGAT
ATGGAATCTGATATTATTCCTGAGGATTATGATGAGAGAGGAGAATTATATGATGATGTT
GATCATCCTCTACCAATAAGCAATCCACTAACAAGCAGTCAACCAATAGATGATGAAATT
TATGAAGAACTTCCAGAAGAAGAAGAGGACAGTGCTCCAGTGAAAGTGGAAGAACAAAGG
AAGATGAGTCAGGATAGTGTCCATCACACCTCAGGGGATAAGAGCACTGATTATGCTAAT
TTTTACCAGGGATTGTGGGATTGTACTGGAGCTTTTTCTGATGAGTTGTCATTTAAGCGT
GGTGATGTGATTTACATTCTTAGCAAGGAATACAATAGATATGGCTGGTGGGTAGGAGAA
ATGAAGGGAGCCATTGGCTTGGTGCCTAAAGCCTACATAATGGAGATGTATGATATT

ORF Protein

Cloned ORF protein sequence for pFN21AE0604 Download

>KIEE0604  359 aa
MPNPSSTSSPYPLPEEIRNLLADVETFVADILKGENLSKKAKEKRESLIKKIKDVKSIYL
QEFQDKGDAEDGEEYDDPFAGPPDTISLASERYDKDDEAPSDGAQFPPIAAQDLPFVLKA
GYLEKRRKDHSFLGFEWQKRWCALSKTVFYYYGSDKDKQQKGEFAIDGYSVRMNNTLRKD
GKKDCCFEISAPDKRIYQFTAASPKDAEEWVQQLKFVLQDMESDIIPEDYDERGELYDDV
DHPLPISNPLTSSQPIDDEIYEELPEEEEDSAPVKVEEQRKMSQDSVHHTSGDKSTDYAN
FYQGLWDCTGAFSDELSFKRGDVIYILSKEYNRYGWWVGEMKGAIGLVPKAYIMEMYDI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0604 Download

>pFN21AE0604  5782 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCCAACCCCAGCAGCACCTCCTCTCCCTACCC
CCTCCCTGAGGAAATTAGGAACCTGTTGGCAGATGTTGAAACATTTGTAGCAGATATACT
GAAAGGAGAAAATTTATCCAAGAAAGCAAAGGAAAAGAGAGAATCCCTTATTAAGAAGAT
AAAAGATGTAAAGTCTATCTATCTTCAGGAATTTCAAGACAAAGGTGATGCAGAAGATGG
GGAAGAATATGATGACCCTTTTGCTGGGCCTCCAGACACTATTTCATTAGCCTCAGAACG
ATATGATAAAGACGATGAAGCCCCCTCTGATGGAGCCCAGTTTCCTCCAATTGCAGCACA
AGACCTTCCTTTTGTTCTAAAGGCTGGCTACCTTGAAAAACGCAGAAAAGATCACAGCTT
TCTGGGATTTGAATGGCAGAAACGGTGGTGTGCTCTCAGTAAAACGGTATTCTATTATTA
TGGAAGTGATAAAGACAAACAACAGAAAGGTGAATTTGCAATAGATGGCTACAGTGTCAG
AATGAATAACACTCTAAGAAAGGATGGAAAGAAAGATTGCTGTTTTGAAATCTCTGCTCC
TGATAAACGTATATATCAGTTTACAGCAGCTTCTCCCAAAGATGCTGAAGAATGGGTACA
GCAGCTGAAATTTGTATTGCAAGATATGGAATCTGATATTATTCCTGAGGATTATGATGA
GAGAGGAGAATTATATGATGATGTTGATCATCCTCTACCAATAAGCAATCCACTAACAAG
CAGTCAACCAATAGATGATGAAATTTATGAAGAACTTCCAGAAGAAGAAGAGGACAGTGC
TCCAGTGAAAGTGGAAGAACAAAGGAAGATGAGTCAGGATAGTGTCCATCACACCTCAGG
GGATAAGAGCACTGATTATGCTAATTTTTACCAGGGATTGTGGGATTGTACTGGAGCTTT
TTCTGATGAGTTGTCATTTAAGCGTGGTGATGTGATTTACATTCTTAGCAAGGAATACAA
TAGATATGGCTGGTGGGTAGGAGAAATGAAGGGAGCCATTGGCTTGGTGCCTAAAGCCTA
CATAATGGAGATGTATGATATTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT