FHC11307

Gene/Protein Characteristic Table for FHC11307

Species

human

Clone Name

pFN21AE0846 Sequence (with vector) Restriction Map

Product ID

FHC11307

Corresponding
Flexi Clone PID

FXC11307

ORF Sequence

DNA sequence (942 bp)
Protein sequence (314 aa)

Linker Type

Flexi type

The extra Val codon is attached to the 3'-end of the ORF, because a Pme I site ...

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529269]

Gene

KIEE0846

Symbol

OR10A3

Alias : HSHTPCRX12, HTPCRX12

Description

olfactory receptor, family 10, subfamily A, member 3

Original Clone ID

cp01246

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 942 bp
Restriction map A Restriction map B

Features of the protein sequence

Description

Length: 314 aa
Result of homology search against nr database ( FASTA output, Multiple alignment) Entry Exp ID% Protein Source P58181 1.8e-126 100.0 Olfactory recep... Homo sapiens XP_001500589 1.1e-114 90.4 similar to Olfa... Equus caballus XP_001251414 2e-114 88.8 similar to Olfa... Bos taurus XP_584199 1.4e-112 88.5 similar to Olfa... Bos taurus XP_854072 1.9e-112 86.6 similar to Olfa... Canis lupus fam... The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 26 50 PR00237 Rhodopsin-like GPCR superfamily IPR000276 59 80 PR00237 Rhodopsin-like GPCR superfamily IPR000725 92 103 PR00245 Olfactory receptor IPR000276 104 126 PR00237 Rhodopsin-like GPCR superfamily IPR000725 129 141 PR00245 Olfactory receptor IPR000725 176 192 PR00245 Olfactory receptor IPR000276 199 222 PR00237 Rhodopsin-like GPCR superfamily IPR000725 236 245 PR00245 Olfactory receptor IPR000276 272 298 PR00237 Rhodopsin-like GPCR superfamily IPR000725 283 294 PR00245 Olfactory receptor HMMPfam IPR000276 41 290 PF00001 Rhodopsin-like GPCR superfamily ProfileScan IPR000276 41 290 PS50262 Rhodopsin-like GPCR superfamily ScanRegExp IPR000276 110 126 PS00237 Rhodopsin-like GPCR superfamily
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 19 NFPELQVQLFGVFLVIYVVTLMG 41 PRIMARY 23 2 61 LFLLNLSVVEVSFSAVITPEMLV 83 PRIMARY 23 3 94 FVGCFAQMYFILLFGGTECFLLG 116 PRIMARY 23 4 136 MNRGVFMKLVIFSWISGIMVATV 158 PRIMARY 23 5 178 FCETPPVLELVCADTFLFEIYAF 200 SECONDARY 23 6 207 VMVPFLLILLSYIRVLFAILKMP 229 PRIMARY 23 7 273 LISLAYTLLTPLLNPLIYSLRNS 295 SECONDARY 23
Result of homology search against disease genes in OMIM database

(FASTA output, Multiple alignment)

H_id	H_len (aa)	OMIM_No.	ID (%)	Q_ali/Q_len (%)
NP_835462	317	608493	83.7	97.8
NP_003688	314	608492	78.6	98.1
NP_006628	314	608496	76.4	97.5
NP_036501	317	608497	78.5	97.5
NP_003691	308	608494	77.5	97.8