PNCK ORF clone FHC11227

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Halotag ORF Clone FHC11227

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Clone info.

Species

human

Clone Name

pFN21AE0672 Sequence (with vector) Restriction Map

Product ID

FHC11227

Flexi Clone PID

FXC11227

ORF Sequence

DNA sequence (1029 bp)
Protein sequence (343 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528904]

Gene

KIEE0672

Symbol

PNCK

Alias : BSTK3, CaMK1b

Description

pregnancy up-regulated nonubiquitous CaM kinase

Original Clone ID

cs02172

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1029 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 343 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS48189.1 2e-80 100.0 PNCK CCDS35503.2 2.3e-80 100.0 PNCK CCDS2582.1 1.4e-51 68.2 CAMK1 CCDS7092.1 1.5e-51 69.4 CAMK1D CCDS7091.1 1.6e-51 69.4 CAMK1D The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017442 15 270 PF00069 Serine/threonine-protein kinase-like domain HMMSmart IPR002290 15 270 SM00220 Serine/threonine-protein kinase domain IPR020635 15 268 SM00219 Tyrosine-protein kinase ProfileScan IPR000719 15 270 PS50011 Protein kinase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 189 YGKAVDVWALGVISYILLCGYP 210 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011529413 343 300680 100.0 100.0 XP_011529414 343 300680 100.0 100.0 XP_011529412 343 300680 100.0 100.0 XP_006724872 343 300680 100.0 100.0 XP_011529409 343 300680 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0672 Download

>KIEE0672  1029 bp
ATGCTGCTGCTGAAGAAACACACGGAGGACATCAGCAGCGTCTACGAGATCCGCGAGAGG
CTCGGCTCGGGTGCCTTCTCCGAGGTGGTGCTGGCCCAGGAGCGGGGCTCCGCACACCTC
GTGGCCCTCAAGTGCATCCCCAAGAAGGCCCTCCGGGGCAAGGAGGCCCTGGTGGAGAAC
GAGATCGCAGTGCTCCGTAGGATCAGTCACCCCAACATCGTCGCTCTGGAGGATGTCCAC
GAGAGCCCTTCCCACCTCTACCTGGCCATGGAACTGGTGACGGGTGGCGAGCTGTTTGAC
CGCATCATGGAGCGCGGCTCCTACACAGAGAAGGATGCCAGCCATCTGGTGGGTCAGGTC
CTTGGCGCCGTCTCCTACCTGCACAGCCTGGGGATCGTGCACCGGGACCTCAAGCCCGAA
AACCTCCTGTATGCCACGCCCTTTGAGGACTCGAAGATCATGGTCTCTGACTTTGGACTC
TCCAAAATCCAGGCTGGGAACATGCTAGGCACCGCCTGTGGGACCCCTGGATATGTGGCC
CCAGAGCTCTTGGAGCAGAAACCCTACGGGAAGGCCGTAGATGTGTGGGCCCTGGGCGTC
ATCTCCTACATCCTGCTGTGTGGGTACCCCCCCTTCTACGACGAGAGCGACCCTGAGCTC
TTCAGCCAGATCCTGAGGGCCAGCTATGAGTTTGACTCTCCTTTCTGGGATGACATCTCA
GAATCAGCCAAAGACTTCATCCGGCACCTTCTGGAGCGAGACCCCCAGAAGAGGTTCACC
TGCCAACAGGCCTTGCGGCACCTTTGGATCTCTGGGGACACAGCCTTCGACAGGGACATC
TTAGGCTCTGTCAGTGAGCAGATCCGGAAGAACTTTGCTCGGACACACTGGAAGCGAGCC
TTCAATGCCACCTCGTTCCTGCGCCACATCCGGAAGCTGGGGCAGATCCCAGAGGGCGAG
GGGGCCTCTGAGCAGGGCATGGCCCGCCACAGCCACTCAGGCCTCCGTGCTGGCCAGCCC
CCCAAGTGG

ORF Protein

Cloned ORF protein sequence for pFN21AE0672 Download

>KIEE0672  343 aa
MLLLKKHTEDISSVYEIRERLGSGAFSEVVLAQERGSAHLVALKCIPKKALRGKEALVEN
EIAVLRRISHPNIVALEDVHESPSHLYLAMELVTGGELFDRIMERGSYTEKDASHLVGQV
LGAVSYLHSLGIVHRDLKPENLLYATPFEDSKIMVSDFGLSKIQAGNMLGTACGTPGYVA
PELLEQKPYGKAVDVWALGVISYILLCGYPPFYDESDPELFSQILRASYEFDSPFWDDIS
ESAKDFIRHLLERDPQKRFTCQQALRHLWISGDTAFDRDILGSVSEQIRKNFARTHWKRA
FNATSFLRHIRKLGQIPEGEGASEQGMARHSHSGLRAGQPPKW

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0672 Download

>pFN21AE0672  5734 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTGCTGCTGAAGAAACACACGGAGGACATCAG
CAGCGTCTACGAGATCCGCGAGAGGCTCGGCTCGGGTGCCTTCTCCGAGGTGGTGCTGGC
CCAGGAGCGGGGCTCCGCACACCTCGTGGCCCTCAAGTGCATCCCCAAGAAGGCCCTCCG
GGGCAAGGAGGCCCTGGTGGAGAACGAGATCGCAGTGCTCCGTAGGATCAGTCACCCCAA
CATCGTCGCTCTGGAGGATGTCCACGAGAGCCCTTCCCACCTCTACCTGGCCATGGAACT
GGTGACGGGTGGCGAGCTGTTTGACCGCATCATGGAGCGCGGCTCCTACACAGAGAAGGA
TGCCAGCCATCTGGTGGGTCAGGTCCTTGGCGCCGTCTCCTACCTGCACAGCCTGGGGAT
CGTGCACCGGGACCTCAAGCCCGAAAACCTCCTGTATGCCACGCCCTTTGAGGACTCGAA
GATCATGGTCTCTGACTTTGGACTCTCCAAAATCCAGGCTGGGAACATGCTAGGCACCGC
CTGTGGGACCCCTGGATATGTGGCCCCAGAGCTCTTGGAGCAGAAACCCTACGGGAAGGC
CGTAGATGTGTGGGCCCTGGGCGTCATCTCCTACATCCTGCTGTGTGGGTACCCCCCCTT
CTACGACGAGAGCGACCCTGAGCTCTTCAGCCAGATCCTGAGGGCCAGCTATGAGTTTGA
CTCTCCTTTCTGGGATGACATCTCAGAATCAGCCAAAGACTTCATCCGGCACCTTCTGGA
GCGAGACCCCCAGAAGAGGTTCACCTGCCAACAGGCCTTGCGGCACCTTTGGATCTCTGG
GGACACAGCCTTCGACAGGGACATCTTAGGCTCTGTCAGTGAGCAGATCCGGAAGAACTT
TGCTCGGACACACTGGAAGCGAGCCTTCAATGCCACCTCGTTCCTGCGCCACATCCGGAA
GCTGGGGCAGATCCCAGAGGGCGAGGGGGCCTCTGAGCAGGGCATGGCCCGCCACAGCCA
CTCAGGCCTCCGTGCTGGCCAGCCCCCCAAGTGGGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT