SRMS ORF clone FHC11009

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11009

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Clone info.

Species

human

Clone Name

pFN21AE0390 Sequence (with vector) Restriction Map

Product ID

FHC11009

Flexi Clone PID

FXC11009

ORF Sequence

DNA sequence (1464 bp)
Protein sequence (488 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527384]

Gene

KIEE0390

Symbol

SRMS

Alias : C20orf148, PTK70, SRM, dJ697K14.1

Description

src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites

Original Clone ID

oc01090

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1464 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 488 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13525.1 3e-101 100.0 SRMS CCDS359.1 6.9e-35 44.5 LCK CCDS54455.1 1.1e-34 41.3 HCK CCDS33460.1 1.1e-34 41.3 HCK CCDS54456.1 1.3e-34 41.5 HCK The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000980 120 134 PR00401 SH2 motif IPR000980 141 151 PR00401 SH2 motif IPR000980 153 164 PR00401 SH2 motif IPR000980 165 175 PR00401 SH2 motif IPR000980 186 200 PR00401 SH2 motif IPR001245 302 315 PR00109 Serine-threonine/tyrosine-protein kinase IPR001245 340 358 PR00109 Serine-threonine/tyrosine-protein kinase IPR001245 388 398 PR00109 Serine-threonine/tyrosine-protein kinase IPR001245 407 429 PR00109 Serine-threonine/tyrosine-protein kinase IPR001245 451 473 PR00109 Serine-threonine/tyrosine-protein kinase HMMPfam IPR000980 120 197 PF00017 SH2 motif IPR001245 232 480 PF07714 Serine-threonine/tyrosine-protein kinase HMMSmart IPR001452 54 111 SM00326 Src homology-3 domain IPR000980 118 203 SM00252 SH2 motif IPR002290 230 484 SM00220 Serine/threonine-protein kinase domain IPR020635 230 480 SM00219 Tyrosine-protein kinase ProfileScan IPR001452 51 112 PS50002 Src homology-3 domain IPR000980 120 212 PS50001 SH2 motif IPR000719 230 488 PS50011 Protein kinase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005347 509 153390,615758 44.5 90.6 NP_001036236 509 153390,615758 44.5 90.6 NP_001165600 505 142370 41.3 93.2 NP_001165604 505 142370 41.3 93.2 NP_001165603 506 142370 41.3 93.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0390 Download

>KIEE0390  1464 bp
ATGGAGCCGTTCCTCAGGAGGCGGCTGGCCTTCCTGTCCTTCTTCTGGGACAAGATCTGG
CCGGCGGGCGGCGAGCCGGACCATGGCACCCCCGGGTCCCTGGACCCCAACACTGACCCA
GTGCCCACGCTCCCCGCCGAGCCTTGCAGCCCCTTCCCTCAGCTCTTCCTTGCGCTCTAT
GACTTCACGGCGCGGTGTGGCGGGGAGCTGAGTGTCCGCCGCGGGGACAGGCTCTGTGCC
CTCGAAGAGGGGGGCGGCTACATCTTCGCACGCAGGCTTTCGGGCCAGCCCAGCGCCGGG
CTCGTGCCCATCACCCACGTGGCCAAGGCTTCTCCTGAGACGCTCTCAGACCAACCCTGG
TACTTTAGCGGGGTCAGTCGGACCCAGGCACAGCAGCTGCTCCTCTCCCCACCCAACGAA
CCAGGGGCCTTCCTCATCCGGCCCAGCGAGAGCAGCCTCGGGGGCTACTCACTGTCAGTC
CGGGCCCAGGCCAAGGTCTGCCACTACCGGGTCTCCATGGCAGCTGATGGCAGCCTCTAC
CTGCAGAAGGGACGGCTCTTTCCCGGCCTGGAGGAGCTGCTCACCTACTACAAGGCCAAC
TGGAAGCTGATCCAGAACCCCCTGCTGCAGCCCTGCATGCCCCAGAAGGCCCCGAGGCAG
GACGTGTGGGAGCGGCCACACTCCGAATTCGCCCTTGGGAGGAAGCTGGGTGAAGGCTAC
TTTGGGGAGGTGTGGGAAGGCCTGTGGCTGGGCTCCCTGCCCGTGGCGATCAAGGTCATC
AAGTCAGCCAACATGAAGCTCACTGACCTCGCCAAGGAGATCCAGACACTGAAGGGCCTG
CGGCACGAGCGGCTCATCCGGCTGCACGCAGTGTGCTCGGGCGGGGAGCCTGTGTACATC
GTCACGGAACTCATGCGCAAGGGGAACCTGCAGGCCTTCCTGGGCACCCCCGAGGGCCGG
GCCCTGCGTCTGCCGCCACTCCTGGGCTTTGCCTGCCAGGTGGCTGAGGGCATGAGCTAC
CTGGAGGAGCAGCGCGTTGTGCACCGGGACTTGGCCGCCCGGAACGTGCTCGTGGACGAC
GGCCTGGCCTGCAAGGTGGCTGACTTCGGCCTGGCCCGGCTGCTCAAGGACGACATCTAC
TCCCCGAGCAGCAGCTCCAAGATCCCGGTCAAGTGGACAGCGCCTGAGGCGGCCAATTAT
CGTGTCTTCTCCCAGAAGTCAGACGTCTGGTCCTTCGGCGTCCTGCTGCACGAGGTTTTC
ACCTATGGCCAGTGTCCCTATGAAGGGATGACCAACCACGAGACGCTGCAGCAGATCATG
CGAGGGTACCGGCTGCCGCGCCCGGCTGCCTGCCCGGCGGAGGTCTACGTGCTCATGCTG
GAGTGCTGGAGGAGCAGCCCCGAGGAACGGCCCTCCTTCGCCACGCTGCGGGAGAAGCTG
CACGCCATCCACAGATGCCACCCC

ORF Protein

Cloned ORF protein sequence for pFN21AE0390 Download

>KIEE0390  488 aa
MEPFLRRRLAFLSFFWDKIWPAGGEPDHGTPGSLDPNTDPVPTLPAEPCSPFPQLFLALY
DFTARCGGELSVRRGDRLCALEEGGGYIFARRLSGQPSAGLVPITHVAKASPETLSDQPW
YFSGVSRTQAQQLLLSPPNEPGAFLIRPSESSLGGYSLSVRAQAKVCHYRVSMAADGSLY
LQKGRLFPGLEELLTYYKANWKLIQNPLLQPCMPQKAPRQDVWERPHSEFALGRKLGEGY
FGEVWEGLWLGSLPVAIKVIKSANMKLTDLAKEIQTLKGLRHERLIRLHAVCSGGEPVYI
VTELMRKGNLQAFLGTPEGRALRLPPLLGFACQVAEGMSYLEEQRVVHRDLAARNVLVDD
GLACKVADFGLARLLKDDIYSPSSSSKIPVKWTAPEAANYRVFSQKSDVWSFGVLLHEVF
TYGQCPYEGMTNHETLQQIMRGYRLPRPAACPAEVYVLMLECWRSSPEERPSFATLREKL
HAIHRCHP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0390 Download

>pFN21AE0390  6169 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGCCGTTCCTCAGGAGGCGGCTGGCCTTCCT
GTCCTTCTTCTGGGACAAGATCTGGCCGGCGGGCGGCGAGCCGGACCATGGCACCCCCGG
GTCCCTGGACCCCAACACTGACCCAGTGCCCACGCTCCCCGCCGAGCCTTGCAGCCCCTT
CCCTCAGCTCTTCCTTGCGCTCTATGACTTCACGGCGCGGTGTGGCGGGGAGCTGAGTGT
CCGCCGCGGGGACAGGCTCTGTGCCCTCGAAGAGGGGGGCGGCTACATCTTCGCACGCAG
GCTTTCGGGCCAGCCCAGCGCCGGGCTCGTGCCCATCACCCACGTGGCCAAGGCTTCTCC
TGAGACGCTCTCAGACCAACCCTGGTACTTTAGCGGGGTCAGTCGGACCCAGGCACAGCA
GCTGCTCCTCTCCCCACCCAACGAACCAGGGGCCTTCCTCATCCGGCCCAGCGAGAGCAG
CCTCGGGGGCTACTCACTGTCAGTCCGGGCCCAGGCCAAGGTCTGCCACTACCGGGTCTC
CATGGCAGCTGATGGCAGCCTCTACCTGCAGAAGGGACGGCTCTTTCCCGGCCTGGAGGA
GCTGCTCACCTACTACAAGGCCAACTGGAAGCTGATCCAGAACCCCCTGCTGCAGCCCTG
CATGCCCCAGAAGGCCCCGAGGCAGGACGTGTGGGAGCGGCCACACTCCGAATTCGCCCT
TGGGAGGAAGCTGGGTGAAGGCTACTTTGGGGAGGTGTGGGAAGGCCTGTGGCTGGGCTC
CCTGCCCGTGGCGATCAAGGTCATCAAGTCAGCCAACATGAAGCTCACTGACCTCGCCAA
GGAGATCCAGACACTGAAGGGCCTGCGGCACGAGCGGCTCATCCGGCTGCACGCAGTGTG
CTCGGGCGGGGAGCCTGTGTACATCGTCACGGAACTCATGCGCAAGGGGAACCTGCAGGC
CTTCCTGGGCACCCCCGAGGGCCGGGCCCTGCGTCTGCCGCCACTCCTGGGCTTTGCCTG
CCAGGTGGCTGAGGGCATGAGCTACCTGGAGGAGCAGCGCGTTGTGCACCGGGACTTGGC
CGCCCGGAACGTGCTCGTGGACGACGGCCTGGCCTGCAAGGTGGCTGACTTCGGCCTGGC
CCGGCTGCTCAAGGACGACATCTACTCCCCGAGCAGCAGCTCCAAGATCCCGGTCAAGTG
GACAGCGCCTGAGGCGGCCAATTATCGTGTCTTCTCCCAGAAGTCAGACGTCTGGTCCTT
CGGCGTCCTGCTGCACGAGGTTTTCACCTATGGCCAGTGTCCCTATGAAGGGATGACCAA
CCACGAGACGCTGCAGCAGATCATGCGAGGGTACCGGCTGCCGCGCCCGGCTGCCTGCCC
GGCGGAGGTCTACGTGCTCATGCTGGAGTGCTGGAGGAGCAGCCCCGAGGAACGGCCCTC
CTTCGCCACGCTGCGGGAGAAGCTGCACGCCATCCACAGATGCCACCCCGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT