PHLDA1 ORF clone FHC10681

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10681

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Clone info.

Species

human

Clone Name

pFN21AB9859 Sequence (with vector) Restriction Map

Product ID

FHC10681

Flexi Clone PID

FXC10681

ORF Sequence

DNA sequence (1200 bp)
Protein sequence (400 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587598]

Gene

KIBB9859

Symbol

PHLDA1

Alias : DT1P1B11, PHRIP, TDAG51

Description

pleckstrin homology-like domain, family A, member 1

Original Clone ID

cp00428

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1200 bp
Restriction map A Restriction map B

Features of the protein sequence

Description

Length: 400 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS31861.1 9.7e-74 99.5 PHLDA1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMSmart IPR001849 150 282 SM00233 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_031376 401 605335 99.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9859 Download

>KIBB9859  1200 bp
ATGAGGCGTGCGCCGGCTGCCGAGCGCCTCTTGGAGCTGGGCTTTTCCCCGCGGTGCGGG
CGCCAGGAGCCGCCTTTTCCGCTGGGTGTCACTCGGGGGTGGGGAAGATGGCCCATTCAA
AAGCGCCGCGAGGGGGCCCGGCCAGTGCCCTTCAGTGAGCGCTCGCAAGAGGACGGCAGA
GGCCCGGCAGCTCGGAGCTCCGGGACCTTGTGGCGCATCAGGACGCGGCTGTCCCTCTGC
CGGGACCCAGAGCCGCCGCCGCCGCTCTGCCTCCTGCGTGTTAGCCTCCTCTGCGCGCTC
CGGGCAGGCGGCCGTGGGAGCCGCTGGGGCGAGGACGGCGCGAGGCTGCTGCTGCTGCCC
CCGGCCCGCGCGGCTGGAAACGGAGAGGCCGAGCCAAGCGGCGGCCCCTCTTATGCTGGG
AGGATGCTGGAGAGTAGCGGCTGCAAAGCGCTGAAGGAGGGCGTGCTGGAGAAGCGCAGC
GACGGGTTGTTGCAGCTCTGGAAGAAAAAGTGTTGCATCCTCACCGAGGAAGGGCTGCTG
CTTATCCCGCCCAAGCAGCTGCAACACCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAG
CAACAACAGCCCGGGCAGGGGCCGGCCGAGCCGTCCCAACCCAGTGGCCCCGCTGTCGCC
AGCCTCGAGCCGCCGGTCAAGCTCAAGGAACTGCACTTCTCCAACATGAAGACCGTGGAC
TGTGTGGAGCGCAAGGGCAAGTACATGTACTTCACTGTGGTGATGGCAGAGGGCAAGGAG
ATCGACTTTCGGTGCCCGCAAGACCAGGGCTGGAACGCCGAGATCACGCTGCAGATGGTG
CAGTACAAGAATCGTCAGGCCATCCTGGCGGTCAAATCCACGCGGCAGAAGCAGCAGCAC
CTGGTCCAGCAGCAGCCCCCCTCGCAGCCGCAGCCGCAGCCGCAGCTCCAGCCCCAACCC
CAGCCTCAGCCTCAGCCGCAACCCCAGCCCCAATCACAACCCCAGCCTCAGCCCCAACCC
AAGCCTCAGCCCCAGCAGCTCCACCCGTATCCGCATCCACATCCACATCCACACTCTCAT
CCTCACTCGCACCCACACCCTCACCCGCACCCGCATCCGCACCAAATACCGCACCCACAC
CCACAGCCGCACTCGCAGCCGCACGGGCACCGGCTTCTCCGCAGCACCTCCAACTCTGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB9859 Download

>KIBB9859  400 aa
MRRAPAAERLLELGFSPRCGRQEPPFPLGVTRGWGRWPIQKRREGARPVPFSERSQEDGR
GPAARSSGTLWRIRTRLSLCRDPEPPPPLCLLRVSLLCALRAGGRGSRWGEDGARLLLLP
PARAAGNGEAEPSGGPSYAGRMLESSGCKALKEGVLEKRSDGLLQLWKKKCCILTEEGLL
LIPPKQLQHQQQQQQQQQQQQQQPGQGPAEPSQPSGPAVASLEPPVKLKELHFSNMKTVD
CVERKGKYMYFTVVMAEGKEIDFRCPQDQGWNAEITLQMVQYKNRQAILAVKSTRQKQQH
LVQQQPPSQPQPQPQLQPQPQPQPQPQPQPQSQPQPQPQPKPQPQQLHPYPHPHPHPHSH
PHSHPHPHPHPHPHQIPHPHPQPHSQPHGHRLLRSTSNSA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9859 Download

>pFN21AB9859  5905 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGGCGTGCGCCGGCTGCCGAGCGCCTCTTGGA
GCTGGGCTTTTCCCCGCGGTGCGGGCGCCAGGAGCCGCCTTTTCCGCTGGGTGTCACTCG
GGGGTGGGGAAGATGGCCCATTCAAAAGCGCCGCGAGGGGGCCCGGCCAGTGCCCTTCAG
TGAGCGCTCGCAAGAGGACGGCAGAGGCCCGGCAGCTCGGAGCTCCGGGACCTTGTGGCG
CATCAGGACGCGGCTGTCCCTCTGCCGGGACCCAGAGCCGCCGCCGCCGCTCTGCCTCCT
GCGTGTTAGCCTCCTCTGCGCGCTCCGGGCAGGCGGCCGTGGGAGCCGCTGGGGCGAGGA
CGGCGCGAGGCTGCTGCTGCTGCCCCCGGCCCGCGCGGCTGGAAACGGAGAGGCCGAGCC
AAGCGGCGGCCCCTCTTATGCTGGGAGGATGCTGGAGAGTAGCGGCTGCAAAGCGCTGAA
GGAGGGCGTGCTGGAGAAGCGCAGCGACGGGTTGTTGCAGCTCTGGAAGAAAAAGTGTTG
CATCCTCACCGAGGAAGGGCTGCTGCTTATCCCGCCCAAGCAGCTGCAACACCAGCAGCA
GCAGCAACAGCAGCAGCAGCAGCAGCAACAACAGCCCGGGCAGGGGCCGGCCGAGCCGTC
CCAACCCAGTGGCCCCGCTGTCGCCAGCCTCGAGCCGCCGGTCAAGCTCAAGGAACTGCA
CTTCTCCAACATGAAGACCGTGGACTGTGTGGAGCGCAAGGGCAAGTACATGTACTTCAC
TGTGGTGATGGCAGAGGGCAAGGAGATCGACTTTCGGTGCCCGCAAGACCAGGGCTGGAA
CGCCGAGATCACGCTGCAGATGGTGCAGTACAAGAATCGTCAGGCCATCCTGGCGGTCAA
ATCCACGCGGCAGAAGCAGCAGCACCTGGTCCAGCAGCAGCCCCCCTCGCAGCCGCAGCC
GCAGCCGCAGCTCCAGCCCCAACCCCAGCCTCAGCCTCAGCCGCAACCCCAGCCCCAATC
ACAACCCCAGCCTCAGCCCCAACCCAAGCCTCAGCCCCAGCAGCTCCACCCGTATCCGCA
TCCACATCCACATCCACACTCTCATCCTCACTCGCACCCACACCCTCACCCGCACCCGCA
TCCGCACCAAATACCGCACCCACACCCACAGCCGCACTCGCAGCCGCACGGGCACCGGCT
TCTCCGCAGCACCTCCAACTCTGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT