SLC35A1 ORF clone FHC10680

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Halotag ORF Clone FHC10680

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Clone info.

Species

human

Clone Name

pFN21AB9921 Sequence (with vector) Restriction Map

Product ID

FHC10680

Flexi Clone PID

FXC10680

ORF Sequence

DNA sequence (1011 bp)
Protein sequence (337 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528735]

Gene

KIBB9921

Symbol

SLC35A1

Alias : CDG2F, CMPST, CST, hCST

Description

solute carrier family 35 (CMP-sialic acid transporter), member A1, transcript variant 1

Original Clone ID

cs01621

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1011 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 337 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5010.1 6.2e-140 100.0 SLC35A1 CCDS55043.1 5.3e-78 82.2 SLC35A1 CCDS43937.1 9.8e-56 46.5 SLC35A2 CCDS14311.1 9.9e-56 46.5 SLC35A2 CCDS75973.1 1e-55 46.5 SLC35A2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007271 74 306 PF04142 Nucleotide-sugar transporter HMMTigr IPR004689 90 313 TIGR00803 UDP-galactose transporter
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 6 DNVTLLFKLYCLAVMTLMAAVYT 28 PRIMARY 23 2 91 LSVPSLVYAVQNNMAFLALSNLD 113 SECONDARY 23 3 132 TVLMLNRTLSKLQWVSVFMLCAG 154 SECONDARY 23 4 174 PLLGFGAIAIAVLCSGFAGVYFE 196 SECONDARY 23 5 211 IQMYLSGIIVTLAGVYLSDGAEI 233 SECONDARY 23 6 241 GYTYYVWFVIFLASVGGLYTSVV 263 PRIMARY 23 7 269 NIMKGFSAAAAIVLSTIASVMLF 291 PRIMARY 23 8 295 ITLTFALGTLLVCVSIYLYGLP 316 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_006407 337 603585,605634 100.0 100.0 NP_001161870 278 603585,605634 82.2 100.0 NP_001035963 393 300896,314375 46.5 92.6 NP_005651 396 300896,314375 46.5 92.6 NP_001269579 406 300896,314375 46.5 92.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9921 Download

>KIBB9921  1011 bp
ATGGCTGCCCCGAGAGACAATGTCACTTTATTATTCAAGTTATACTGCTTGGCAGTGATG
ACCCTGATGGCTGCAGTCTATACCATAGCTTTAAGATACACAAGGACATCAGACAAAGAA
CTCTACTTTTCAACCACAGCCGTGTGTATCACAGAAGTTATAAAGTTATTGCTAAGTGTG
GGAATTTTAGCTAAAGAAACTGGTAGTCTGGGTAGATTCAAAGCATCTTTAAGAGAAAAT
GTCTTGGGGAGCCCCAAGGAACTGTTGAAGTTAAGTGTGCCATCGTTAGTGTATGCTGTT
CAGAACAACATGGCTTTCCTAGCTCTTAGCAATCTGGATGCAGCAGTGTACCAGGTGACC
TACCAGTTGAAGATTCCGTGTACTGCTTTATGCACTGTTTTAATGTTAAACCGGACACTC
AGCAAATTACAGTGGGTTTCAGTTTTTATGCTGTGTGCTGGAGTTACGCTTGTACAGTGG
AAACCAGCCCAAGCTACAAAAGTGGTGGTGGAACAAAATCCATTATTAGGGTTTGGCGCT
ATAGCTATTGCTGTATTGTGCTCAGGATTTGCAGGAGTATATTTTGAAAAAGTTTTAAAG
AGTTCAGATACTTCTCTTTGGGTGAGAAACATTCAAATGTATCTATCAGGGATTATTGTG
ACATTAGCTGGCGTCTACTTGTCAGATGGAGCTGAAATTAAAGAAAAAGGATTTTTCTAT
GGTTACACATATTATGTCTGGTTTGTCATCTTTCTTGCAAGTGTTGGTGGCCTCTACACT
TCTGTTGTGGTTAAGTACACAGACAACATCATGAAAGGCTTTTCTGCAGCAGCGGCCATT
GTCCTTTCCACCATTGCTTCAGTAATGCTGTTTGGATTACAGATAACACTCACCTTTGCC
CTGGGTACTCTTCTTGTATGTGTTTCCATATATCTCTATGGATTACCCAGACAAGACACT
ACATCCATCCAACAAGGAGAAACAGCTTCAAAGGAGAGAGTTATTGGTGTG

ORF Protein

Cloned ORF protein sequence for pFN21AB9921 Download

>KIBB9921  337 aa
MAAPRDNVTLLFKLYCLAVMTLMAAVYTIALRYTRTSDKELYFSTTAVCITEVIKLLLSV
GILAKETGSLGRFKASLRENVLGSPKELLKLSVPSLVYAVQNNMAFLALSNLDAAVYQVT
YQLKIPCTALCTVLMLNRTLSKLQWVSVFMLCAGVTLVQWKPAQATKVVVEQNPLLGFGA
IAIAVLCSGFAGVYFEKVLKSSDTSLWVRNIQMYLSGIIVTLAGVYLSDGAEIKEKGFFY
GYTYYVWFVIFLASVGGLYTSVVVKYTDNIMKGFSAAAAIVLSTIASVMLFGLQITLTFA
LGTLLVCVSIYLYGLPRQDTTSIQQGETASKERVIGV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9921 Download

>pFN21AB9921  5716 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTGCCCCGAGAGACAATGTCACTTTATTATT
CAAGTTATACTGCTTGGCAGTGATGACCCTGATGGCTGCAGTCTATACCATAGCTTTAAG
ATACACAAGGACATCAGACAAAGAACTCTACTTTTCAACCACAGCCGTGTGTATCACAGA
AGTTATAAAGTTATTGCTAAGTGTGGGAATTTTAGCTAAAGAAACTGGTAGTCTGGGTAG
ATTCAAAGCATCTTTAAGAGAAAATGTCTTGGGGAGCCCCAAGGAACTGTTGAAGTTAAG
TGTGCCATCGTTAGTGTATGCTGTTCAGAACAACATGGCTTTCCTAGCTCTTAGCAATCT
GGATGCAGCAGTGTACCAGGTGACCTACCAGTTGAAGATTCCGTGTACTGCTTTATGCAC
TGTTTTAATGTTAAACCGGACACTCAGCAAATTACAGTGGGTTTCAGTTTTTATGCTGTG
TGCTGGAGTTACGCTTGTACAGTGGAAACCAGCCCAAGCTACAAAAGTGGTGGTGGAACA
AAATCCATTATTAGGGTTTGGCGCTATAGCTATTGCTGTATTGTGCTCAGGATTTGCAGG
AGTATATTTTGAAAAAGTTTTAAAGAGTTCAGATACTTCTCTTTGGGTGAGAAACATTCA
AATGTATCTATCAGGGATTATTGTGACATTAGCTGGCGTCTACTTGTCAGATGGAGCTGA
AATTAAAGAAAAAGGATTTTTCTATGGTTACACATATTATGTCTGGTTTGTCATCTTTCT
TGCAAGTGTTGGTGGCCTCTACACTTCTGTTGTGGTTAAGTACACAGACAACATCATGAA
AGGCTTTTCTGCAGCAGCGGCCATTGTCCTTTCCACCATTGCTTCAGTAATGCTGTTTGG
ATTACAGATAACACTCACCTTTGCCCTGGGTACTCTTCTTGTATGTGTTTCCATATATCT
CTATGGATTACCCAGACAAGACACTACATCCATCCAACAAGGAGAAACAGCTTCAAAGGA
GAGAGTTATTGGTGTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT