NSUN4 ORF clone FHC10582

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10582

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Clone info.

Species

human

Clone Name

pFN21AB6510 Sequence (with vector) Restriction Map

Product ID

FHC10582

Flexi Clone PID

FXC10582

ORF Sequence

DNA sequence (1152 bp)
Protein sequence (384 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528648]

Gene

KIBB6510

Symbol

NSUN4

Alias : SHTAP

Description

NOP2/Sun domain family, member 4, transcript variant 1

Original Clone ID

cs01110

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1152 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 384 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS534.1 3.3e-170 100.0 NSUN4 CCDS57996.1 1.8e-148 100.0 NSUN4 CCDS2927.1 3.7e-32 35.4 NSUN3 CCDS58202.1 2.7e-11 33.3 NOP2 CCDS44811.1 3.4e-11 33.3 NOP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR023267 178 188 PR02008 RNA (C5-cytosine) methyltransferase IPR023267 250 262 PR02008 RNA (C5-cytosine) methyltransferase IPR023267 302 318 PR02008 RNA (C5-cytosine) methyltransferase HMMPfam IPR001678 155 325 PF01189 Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_950245 384 615394 100.0 100.0 NP_001243056 335 615394 100.0 87.2 NP_001243057 335 615394 100.0 87.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6510 Download

>KIBB6510  1152 bp
ATGGCTGCGCTGACACTGAGGGGTGTCCGGGAGCTGCTGAAGCGTGTGGACCTCGCGACG
GTCCCGCGGAGACATCGATATAAGAAGAAATGGGCTGCCACAGAGCCCAAATTCCCTGCT
GTTCGACTGGCTTTGCAGAATTTTGACATGACTTACAGTGTGCAGTTTGGAGATCTTTGG
CCATCAATCCGTGTCAGTCTCCTCTCAGAGCAGAAGTATGGTGCACTGGTCAATAACTTT
GCTGCCTGGGATCATGTAAGTGCTAAGCTGGAGCAGCTGAGTGCCAAGGATTTTGTGAAT
GAAGCCATCTCCCACTGGGAACTGCAGTCTGAGGGTGGCCAATCTGCAGCCCCATCCCCT
GCCTCCTGGGCCTGCAGTCCGAACCTTCGATGCTTCACTTTTGACAGAGGGGATATCAGT
CGCTTCCCTCCTGCCAGACCTGGCAGCCTGGGTGTCATGGAGTACTACCTGATGGATGCT
GCCTCCTTGCTGCCTGTTCTGGCCCTCGGCCTGCAGCCTGGGGACATCGTGCTTGACCTA
TGTGCAGCTCCTGGGGGAAAGACACTAGCGTTGCTTCAGACTGGCTGTTGCCGCAATCTT
GCTGCCAATGATCTCTCCCCGTCCCGAATAGCCAGACTACAGAAGATCCTTCACAGCTAT
GTGCCTGAAGAGATCAGGGATGGAAATCAAGTTCGAGTTACCTCATGGGATGGCAGGAAA
TGGGGAGAACTGGAGGGGGACACCTATGACCGGGTGCTGGTGGATGTGCCCTGTACCACA
GACCGCCACTCCCTTCATGAGGAGGAGAACAACATCTTTAAGCGGTCAAGGAAGAAGGAG
CGACAGATATTGCCTGTGCTGCAAGTGCAGCTTCTTGCGGCTGGACTCCTTGCCACCAAA
CCAGGAGGCCATGTTGTCTATTCTACCTGCTCACTCTCACACTTACAGAACGAGTATGTG
GTGCAAGGTGCCATTGAGCTCCTGGCCAATCAATACAGCATCCAGGTACAGGTGGAAGAT
CTGACTCACTTCCGAAGGGTTTTCATGGACACATTTTGTTTCTTCTCATCCTGTCAGGTT
GGGGAGCTGGTAATACCAAACCTCATGGCCAATTTTGGCCCCATGTACTTCTGCAAAATG
CGTAGGCTGACA

ORF Protein

Cloned ORF protein sequence for pFN21AB6510 Download

>KIBB6510  384 aa
MAALTLRGVRELLKRVDLATVPRRHRYKKKWAATEPKFPAVRLALQNFDMTYSVQFGDLW
PSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSP
ASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDL
CAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRK
WGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQILPVLQVQLLAAGLLATK
PGGHVVYSTCSLSHLQNEYVVQGAIELLANQYSIQVQVEDLTHFRRVFMDTFCFFSSCQV
GELVIPNLMANFGPMYFCKMRRLT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6510 Download

>pFN21AB6510  5857 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTGCGCTGACACTGAGGGGTGTCCGGGAGCT
GCTGAAGCGTGTGGACCTCGCGACGGTCCCGCGGAGACATCGATATAAGAAGAAATGGGC
TGCCACAGAGCCCAAATTCCCTGCTGTTCGACTGGCTTTGCAGAATTTTGACATGACTTA
CAGTGTGCAGTTTGGAGATCTTTGGCCATCAATCCGTGTCAGTCTCCTCTCAGAGCAGAA
GTATGGTGCACTGGTCAATAACTTTGCTGCCTGGGATCATGTAAGTGCTAAGCTGGAGCA
GCTGAGTGCCAAGGATTTTGTGAATGAAGCCATCTCCCACTGGGAACTGCAGTCTGAGGG
TGGCCAATCTGCAGCCCCATCCCCTGCCTCCTGGGCCTGCAGTCCGAACCTTCGATGCTT
CACTTTTGACAGAGGGGATATCAGTCGCTTCCCTCCTGCCAGACCTGGCAGCCTGGGTGT
CATGGAGTACTACCTGATGGATGCTGCCTCCTTGCTGCCTGTTCTGGCCCTCGGCCTGCA
GCCTGGGGACATCGTGCTTGACCTATGTGCAGCTCCTGGGGGAAAGACACTAGCGTTGCT
TCAGACTGGCTGTTGCCGCAATCTTGCTGCCAATGATCTCTCCCCGTCCCGAATAGCCAG
ACTACAGAAGATCCTTCACAGCTATGTGCCTGAAGAGATCAGGGATGGAAATCAAGTTCG
AGTTACCTCATGGGATGGCAGGAAATGGGGAGAACTGGAGGGGGACACCTATGACCGGGT
GCTGGTGGATGTGCCCTGTACCACAGACCGCCACTCCCTTCATGAGGAGGAGAACAACAT
CTTTAAGCGGTCAAGGAAGAAGGAGCGACAGATATTGCCTGTGCTGCAAGTGCAGCTTCT
TGCGGCTGGACTCCTTGCCACCAAACCAGGAGGCCATGTTGTCTATTCTACCTGCTCACT
CTCACACTTACAGAACGAGTATGTGGTGCAAGGTGCCATTGAGCTCCTGGCCAATCAATA
CAGCATCCAGGTACAGGTGGAAGATCTGACTCACTTCCGAAGGGTTTTCATGGACACATT
TTGTTTCTTCTCATCCTGTCAGGTTGGGGAGCTGGTAATACCAAACCTCATGGCCAATTT
TGGCCCCATGTACTTCTGCAAAATGCGTAGGCTGACAGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT