SLC25A22 ORF clone FHC10435

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Halotag ORF Clone FHC10435

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Clone info.

Species

human

Clone Name

pFN21AB4917 Sequence (with vector) Restriction Map

Product ID

FHC10435

Flexi Clone PID

FXC10435

Original Clone PID

ORK10435

ORF Sequence

DNA sequence (969 bp)
Protein sequence (323 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528574]

Gene

KIBB4917

Symbol

SLC25A22

Alias : DEE3, EIEE3, GC-1, GC1, NET44

Description

solute carrier family 25 (mitochondrial carrier: glutamate), member 22, transcript variant 2

Original Clone ID

pf02762

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 969 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 323 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7715.1 8.3e-130 100.0 SLC25A22 CCDS13744.1 2.4e-81 65.6 SLC25A18 CCDS9959.1 7.1e-21 30.2 SLC25A47 CCDS5645.1 5.4e-19 43.3 SLC25A13 CCDS55130.1 5.4e-19 43.3 SLC25A13 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002067 11 24 PR00926 Mitochondrial carrier protein IPR002067 24 38 PR00926 Mitochondrial carrier protein IPR002067 68 88 PR00926 Mitochondrial carrier protein IPR002067 187 205 PR00926 Mitochondrial carrier protein IPR002067 232 254 PR00926 Mitochondrial carrier protein HMMPfam IPR018108 5 97 PF00153 Mitochondrial substrate/solute carrier IPR018108 100 212 PF00153 Mitochondrial substrate/solute carrier IPR018108 222 306 PF00153 Mitochondrial substrate/solute carrier ProfileScan IPR018108 6 93 PS50920 Mitochondrial substrate/solute carrier IPR018108 101 214 PS50920 Mitochondrial substrate/solute carrier IPR018108 223 312 PS50920 Mitochondrial substrate/solute carrier
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 9 PAKLINGGIAGLIGVTCVFPIDL 31 SECONDARY 23 2 294 VIAPLFGIAQVVYFLGIAESLLG 316 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001177989 323 609302,609304 100.0 100.0 NP_078974 323 609302,609304 100.0 100.0 XP_011518671 323 609302,609304 100.0 100.0 NP_001177990 323 609302,609304 100.0 100.0 XP_011518672 323 609302,609304 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4917 Download

>KIBB4917  969 bp
ATGGCTGATAAGCAGATCAGCCTGCCAGCCAAGCTCATCAATGGCGGCATCGCCGGGCTG
ATCGGTGTCACCTGCGTGTTTCCCATCGACCTGGCCAAGACCAGGCTGCAGAACCAGCAG
AACGGCCAGCGCGTGTACACGAGCATGTCCGACTGCCTCATCAAGACCGTCCGCTCCGAG
GGCTACTTCGGCATGTACCGGGGAGCTGCTGTGAACTTGACCCTCGTCACCCCCGAGAAG
GCCATCAAGCTGGCAGCCAACGACTTCTTCCGACATCAGCTCTCTAAGGACGGGCAGAAG
CTGACCCTGCTTAAAGAGATGCTGGCGGGCTGTGGGGCTGGCACCTGCCAGGTGATCGTG
ACCACGCCCATGGAGATGCTGAAGATCCAGCTGCAGGATGCAGGGCGCATTGCCGCCCAG
AGGAAGATCCTGGCTGCCCAGGGCCAGCTCTCGGCCCAGGGGGGTGCCCAGCCCTCAGTG
GAGGCTCCAGCTGCCCCTCGGCCCACGGCCACCCAGCTGACCCGCGACCTGCTGCGGAGC
CGTGGCATTGCCGGTCTCTACAAGGGACTCGGGGCCACGCTGCTCAGGGATGTCCCCTTC
TCTGTGGTGTACTTCCCGCTCTTTGCCAACCTGAACCAGCTGGGCCGCCCGGCGTCCGAG
GAGAAGTCGCCTTTCTACGTGTCCTTCCTGGCCGGCTGTGTGGCTGGGAGTGCCGCCGCT
GTGGCCGTCAACCCCTGTGATGTGGTGAAGACGCGGCTCCAGTCACTTCAGCGAGGCGTC
AACGAGGACACCTACTCTGGGATCCTGGACTGTGCCAGGAAGATCCTGCGGCACGAGGGC
CCCTCGGCCTTCCTGAAGGGCGCCTACTGCCGCGCGCTGGTCATCGCGCCCCTTTTCGGC
ATCGCACAGGTGGTCTACTTCCTGGGCATCGCGGAGTCCCTGCTGGGGCTGCTGCAGGAC
CCCCAGGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB4917 Download

>KIBB4917  323 aa
MADKQISLPAKLINGGIAGLIGVTCVFPIDLAKTRLQNQQNGQRVYTSMSDCLIKTVRSE
GYFGMYRGAAVNLTLVTPEKAIKLAANDFFRHQLSKDGQKLTLLKEMLAGCGAGTCQVIV
TTPMEMLKIQLQDAGRIAAQRKILAAQGQLSAQGGAQPSVEAPAAPRPTATQLTRDLLRS
RGIAGLYKGLGATLLRDVPFSVVYFPLFANLNQLGRPASEEKSPFYVSFLAGCVAGSAAA
VAVNPCDVVKTRLQSLQRGVNEDTYSGILDCARKILRHEGPSAFLKGAYCRALVIAPLFG
IAQVVYFLGIAESLLGLLQDPQA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4917 Download

>pFN21AB4917  5674 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTGATAAGCAGATCAGCCTGCCAGCCAAGCT
CATCAATGGCGGCATCGCCGGGCTGATCGGTGTCACCTGCGTGTTTCCCATCGACCTGGC
CAAGACCAGGCTGCAGAACCAGCAGAACGGCCAGCGCGTGTACACGAGCATGTCCGACTG
CCTCATCAAGACCGTCCGCTCCGAGGGCTACTTCGGCATGTACCGGGGAGCTGCTGTGAA
CTTGACCCTCGTCACCCCCGAGAAGGCCATCAAGCTGGCAGCCAACGACTTCTTCCGACA
TCAGCTCTCTAAGGACGGGCAGAAGCTGACCCTGCTTAAAGAGATGCTGGCGGGCTGTGG
GGCTGGCACCTGCCAGGTGATCGTGACCACGCCCATGGAGATGCTGAAGATCCAGCTGCA
GGATGCAGGGCGCATTGCCGCCCAGAGGAAGATCCTGGCTGCCCAGGGCCAGCTCTCGGC
CCAGGGGGGTGCCCAGCCCTCAGTGGAGGCTCCAGCTGCCCCTCGGCCCACGGCCACCCA
GCTGACCCGCGACCTGCTGCGGAGCCGTGGCATTGCCGGTCTCTACAAGGGACTCGGGGC
CACGCTGCTCAGGGATGTCCCCTTCTCTGTGGTGTACTTCCCGCTCTTTGCCAACCTGAA
CCAGCTGGGCCGCCCGGCGTCCGAGGAGAAGTCGCCTTTCTACGTGTCCTTCCTGGCCGG
CTGTGTGGCTGGGAGTGCCGCCGCTGTGGCCGTCAACCCCTGTGATGTGGTGAAGACGCG
GCTCCAGTCACTTCAGCGAGGCGTCAACGAGGACACCTACTCTGGGATCCTGGACTGTGC
CAGGAAGATCCTGCGGCACGAGGGCCCCTCGGCCTTCCTGAAGGGCGCCTACTGCCGCGC
GCTGGTCATCGCGCCCCTTTTCGGCATCGCACAGGTGGTCTACTTCCTGGGCATCGCGGA
GTCCCTGCTGGGGCTGCTGCAGGACCCCCAGGCCGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT