DENND1B ORF clone FHC10163

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10163

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Clone info.

Species

human

Clone Name

pFN21AE3928 Sequence (with vector) Restriction Map

Product ID

FHC10163

ORF Sequence

DNA sequence (1188 bp)
Protein sequence (396 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3928

Symbol

DENND1B

Alias : C1ORF18, C1orf218, FAM31B

Description

DENN/MADD domain containing 1B, transcript variant 5

Original Clone ID

cp93928

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1188 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 396 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS72996.1 4.9e-176 99.7 DENND1B CCDS35134.1 8.9e-120 70.6 DENND1A CCDS35133.1 1.5e-119 70.6 DENND1A CCDS41452.2 7.3e-110 94.5 DENND1B CCDS72997.1 7.1e-104 93.6 DENND1B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR005113 12 77 PF03456 uDENN IPR001194 84 261 PF02141 DENN IPR005112 294 358 PF03455 dDENN HMMSmart IPR005113 1 79 SM00800 uDENN IPR001194 80 261 SM00799 DENN IPR005112 292 358 SM00801 dDENN ProfileScan IPR005113 12 79 PS50946 uDENN IPR001194 80 261 PS50211 DENN IPR005112 292 358 PS50947 dDENN
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001287787 396 613292 99.7 100.0 XP_016855960 745 613292 99.0 97.7 XP_005244988 755 613292 98.6 93.4 XP_011507549 803 613292 98.6 93.4 NP_079096 559 613633 70.6 95.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3928 Download

>KIEE3928  1188 bp
ATGGCGGCAGCTCCACGGGAGGAGAAAAGATGGCCCCAACCTGTATTTTCGAATCCTGTG
GTATTGTGGAAATTCCCAGAGGACTTTGGAGACCAGGAAATACTACAGAGTGTGCCAAAG
TTCTGTTTTCCCTTTGACGTTGAAAGGGTGTCTCAGAATCAAGTTGGACAGCACTTTACC
TTTGTACTGACAGACATTGAAAGTAAACAGAGATTTGGATTCTGCAGACTGACGTCAGGA
GGCACAATTTGTTTATGCATCCTTAGTTACCTTCCCTGGTTTGAAGTGTATTACAAGCTT
CTAAATACTCTTGCAGATTACTTGGCTAAGGAACTGGAAAATGATTTGAATGAAACTCTC
AGATCACTGTATAACCACCCAGTACCAAAGGCAAATACTCCTGTAAATTTGAGTGTGCAT
TCCTACTTCATTGCCCCTGATGTAACTGGACTCCCAACAATACCCGAGAGTAGAAATCTT
ACAGAATATTTTGTTGCCGTGGATGTGAACAACATGCTGCAGCTGTATGCCAGTATGCTG
CATGAAAGGCGCATCGTGATTATCTCGAGCAAATTAAGCACTTTAACTGCCTGTATCCAT
GGATCAGCTGCTCTTCTATACCCAATGTATTGGCAACACATATACATCCCAGTGCTTCCT
CCACACCTGCTGGACTACTGCTGTGCCCCAATGCCATACCTGATTGGAATACACTCCAGC
CTCATAGAGAGAGTGAAAAACAAATCATTGGAAGATGTTGTTATGTTAAATGTTGATACA
AACACATTAGAATCACCATTTAGTGACTTGAACAACCTACCAAGTGATGTGGTCTCGGCC
TTGAAAAATAAACTGAAGAAGCAGTCTACAGCTACGGGTGATGGAGTAGCTAGGGCCTTT
CTTAGAGCACAGGCTGCTTTGTTTGGATACTACAGAGATGCACTGAGATACAAACCTGGT
GAGCCCATCACTTTCTGTGAGGAGAGTTTTGTAAAGCACCGCTCAAGCGTGATGAAACAG
TTCCTGGAAACTGCCATTAACCTCCAGCTTTTTAAGCAGTTTATCGATGGTCGACTGGCA
AAACTAAATGCAGGAAGGGGTTTCTCTGATGTATTTGAAGAAGAGATCACTTCAGGTGGC
TTTTGTGGAGGTAAAGACAAGTTACAATATGATTATCCATTTTCTCAA

ORF Protein

Cloned ORF protein sequence for pFN21AE3928 Download

>KIEE3928  396 aa
MAAAPREEKRWPQPVFSNPVVLWKFPEDFGDQEILQSVPKFCFPFDVERVSQNQVGQHFT
FVLTDIESKQRFGFCRLTSGGTICLCILSYLPWFEVYYKLLNTLADYLAKELENDLNETL
RSLYNHPVPKANTPVNLSVHSYFIAPDVTGLPTIPESRNLTEYFVAVDVNNMLQLYASML
HERRIVIISSKLSTLTACIHGSAALLYPMYWQHIYIPVLPPHLLDYCCAPMPYLIGIHSS
LIERVKNKSLEDVVMLNVDTNTLESPFSDLNNLPSDVVSALKNKLKKQSTATGDGVARAF
LRAQAALFGYYRDALRYKPGEPITFCEESFVKHRSSVMKQFLETAINLQLFKQFIDGRLA
KLNAGRGFSDVFEEEITSGGFCGGKDKLQYDYPFSQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3928 Download

>pFN21AE3928  5893 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCAGCTCCACGGGAGGAGAAAAGATGGCC
CCAACCTGTATTTTCGAATCCTGTGGTATTGTGGAAATTCCCAGAGGACTTTGGAGACCA
GGAAATACTACAGAGTGTGCCAAAGTTCTGTTTTCCCTTTGACGTTGAAAGGGTGTCTCA
GAATCAAGTTGGACAGCACTTTACCTTTGTACTGACAGACATTGAAAGTAAACAGAGATT
TGGATTCTGCAGACTGACGTCAGGAGGCACAATTTGTTTATGCATCCTTAGTTACCTTCC
CTGGTTTGAAGTGTATTACAAGCTTCTAAATACTCTTGCAGATTACTTGGCTAAGGAACT
GGAAAATGATTTGAATGAAACTCTCAGATCACTGTATAACCACCCAGTACCAAAGGCAAA
TACTCCTGTAAATTTGAGTGTGCATTCCTACTTCATTGCCCCTGATGTAACTGGACTCCC
AACAATACCCGAGAGTAGAAATCTTACAGAATATTTTGTTGCCGTGGATGTGAACAACAT
GCTGCAGCTGTATGCCAGTATGCTGCATGAAAGGCGCATCGTGATTATCTCGAGCAAATT
AAGCACTTTAACTGCCTGTATCCATGGATCAGCTGCTCTTCTATACCCAATGTATTGGCA
ACACATATACATCCCAGTGCTTCCTCCACACCTGCTGGACTACTGCTGTGCCCCAATGCC
ATACCTGATTGGAATACACTCCAGCCTCATAGAGAGAGTGAAAAACAAATCATTGGAAGA
TGTTGTTATGTTAAATGTTGATACAAACACATTAGAATCACCATTTAGTGACTTGAACAA
CCTACCAAGTGATGTGGTCTCGGCCTTGAAAAATAAACTGAAGAAGCAGTCTACAGCTAC
GGGTGATGGAGTAGCTAGGGCCTTTCTTAGAGCACAGGCTGCTTTGTTTGGATACTACAG
AGATGCACTGAGATACAAACCTGGTGAGCCCATCACTTTCTGTGAGGAGAGTTTTGTAAA
GCACCGCTCAAGCGTGATGAAACAGTTCCTGGAAACTGCCATTAACCTCCAGCTTTTTAA
GCAGTTTATCGATGGTCGACTGGCAAAACTAAATGCAGGAAGGGGTTTCTCTGATGTATT
TGAAGAAGAGATCACTTCAGGTGGCTTTTGTGGAGGTAAAGACAAGTTACAATATGATTA
TCCATTTTCTCAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT