RHAG ORF clone FHC09997

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Halotag ORF Clone FHC09997

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Clone info.

Species

human

Clone Name

pFN21AE1418 Sequence (with vector) Restriction Map

Product ID

FHC09997

ORF Sequence

DNA sequence (1227 bp)
Protein sequence (409 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590393]

Gene

KIEE1418

Symbol

RHAG

Alias : CD241, OHS, OHST, RH2, RH50A, RHNR, Rh50, Rh50GP, SLC42A1

Description

Rh-associated glycoprotein

Original Clone ID

oc01418

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1227 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 409 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4927.1 1.4e-172 99.5 RHAG CCDS10351.1 2.3e-85 50.7 RHCG CCDS41414.2 4.5e-81 50.8 RHBG CCDS262.1 7.8e-46 36.5 RHD CCDS30635.1 6.8e-45 36.1 RHCE The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002229 4 22 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 51 68 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 80 97 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 117 133 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 140 163 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 174 189 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 209 227 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 239 261 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 274 287 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 297 315 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 335 351 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 366 391 PR00342 Blood group Rhesus C/E/D polypeptide HMMPfam IPR024041 15 402 PF00909 Ammonium transporter AmtB-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 4 TFPLMAIVLEIAMIVLFGLFVEY 26 PRIMARY 23 2 76 GFSSVGINLLVAALGLQWGTIVQ 98 PRIMARY 23 3 111 GIKNMINADFSAATVLISFGAVL 133 SECONDARY 23 4 141 MLIMTILEIVFFAHNEYLVSEIF 163 SECONDARY 23 5 171 SMTIHAFGAYFGLAVAGILYRSG 193 SECONDARY 23 6 206 YSDLFAMIGTLFLWMFWPSFNSA 228 SECONDARY 23 7 239 AIVNTYFSLAACVLTAFAFSSLV 261 PRIMARY 23 8 284 GTCADMAIHPFGSMIIGSIAGMV 306 SECONDARY 23 9 333 LHGLPGVVGGLAGIVAVAMGASN 355 SECONDARY 23 10 363 AALGSSIGTAVVGGLMTGLILKL 385 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000315 409 180297,185000,268150 99.5 100.0 NP_001307970 479 605381 50.7 99.5 NP_057405 479 605381 50.7 99.5 XP_016857347 414 607079 50.9 93.2 XP_016857346 428 607079 50.1 97.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1418 Download

>KIEE1418  1227 bp
ATGAGGTTCACATTCCCTCTCATGGCTATAGTCCTGGAAATTGCCATGATTGTTTTATTT
GGATTATTTGTTGAGTATGAAACGGACCAGACTGTTCTCGAGCAGCTCAACATCACCAAG
CCAACAGACATGGGCATATTCTTTGAGTTATATCCTCTGTTCCAAGATGTACATGTTATG
ATATTTGTTGGGTTTGGCTTCCTCATGACCTTCCTGAAGAAATATGGCTTCAGCAGTGTG
GGTATCAACCTACTCGTTGCTGCTTTGGGCCTCCAGTGGGGCACTATTGTACAGGGAACC
CTGCAAAGCCAGGGACAGAAATTTAACATTGGAATCAAAAACATGATAAATGCAGACTTC
AGTGCAGCCACAGTTCTGATATCTTTTGGAGCTGTCCTGGGAAAAACGAGCCCCACCCAA
ATGCTGATCATGACAATTTTAGAAATTGTTTTCTTTGCCCACAATGAATACCTGGTTAGT
GAAATATTTAAGGCCTCTGACATTGGAGCATCAATGACGATCCATGCCTTTGGGGCCTAC
TTTGGCTTGGCTGTAGCAGGCATCTTGTATCGATCTGGACTGAGAAAGGGGCGTGAAAAT
GAAGAGTCCGCATACTACTCAGACTTGTTTGCAATGATTGGGACTCTCTTTCTGTGGATG
TTTTGGCCCAGCTTTAACTCGGCCATTGCTGAACCTGGAGACAAACAGTGCAGGGCCATT
GTAAACACGTACTTCTCTCTCGCTGCCTGTGTGCTCACAGCCTTTGCCTTCTCCAGCCTA
GTGGAGCACCGAGGCAAGCTCAACATGGTTCACATTCAGAATGCCACCCTTGCTGGAGGA
GTTGCTGTGGGCACTTGTGCGGATATGGCAATTCACCCATTTGGTTCTATGATTATTGGG
AGCATTGCAGGAATGGTCTCTGTGCTTGGATACAAGTTCCTGACTCCACTTTTTACTACT
AAACTGAGGATCCATGATACATGTGGGGTCCATAACCTCCACGGCTTACCTGGTGTAGTG
GGAGGCCTTGCAGGCATTGTGGCAGTAGCAATGGGCGCCTCCAACACGTCTATGGCCATG
CAGGCAGCTGCACTGGGTTCCTCTATCGGAACAGCAGTTGTTGGAGGTCTGATGACAGGT
TTAATTCTAAAGTTGCCTCTCTGGGGACAGCCATCTGACCAGAACTGCTATGATGATTCT
GTTTATTGGAAGGTCCCTAAGACGAGA

ORF Protein

Cloned ORF protein sequence for pFN21AE1418 Download

>KIEE1418  409 aa
MRFTFPLMAIVLEIAMIVLFGLFVEYETDQTVLEQLNITKPTDMGIFFELYPLFQDVHVM
IFVGFGFLMTFLKKYGFSSVGINLLVAALGLQWGTIVQGTLQSQGQKFNIGIKNMINADF
SAATVLISFGAVLGKTSPTQMLIMTILEIVFFAHNEYLVSEIFKASDIGASMTIHAFGAY
FGLAVAGILYRSGLRKGRENEESAYYSDLFAMIGTLFLWMFWPSFNSAIAEPGDKQCRAI
VNTYFSLAACVLTAFAFSSLVEHRGKLNMVHIQNATLAGGVAVGTCADMAIHPFGSMIIG
SIAGMVSVLGYKFLTPLFTTKLRIHDTCGVHNLHGLPGVVGGLAGIVAVAMGASNTSMAM
QAAALGSSIGTAVVGGLMTGLILKLPLWGQPSDQNCYDDSVYWKVPKTR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1418 Download

>pFN21AE1418  5932 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGGTTCACATTCCCTCTCATGGCTATAGTCCT
GGAAATTGCCATGATTGTTTTATTTGGATTATTTGTTGAGTATGAAACGGACCAGACTGT
TCTCGAGCAGCTCAACATCACCAAGCCAACAGACATGGGCATATTCTTTGAGTTATATCC
TCTGTTCCAAGATGTACATGTTATGATATTTGTTGGGTTTGGCTTCCTCATGACCTTCCT
GAAGAAATATGGCTTCAGCAGTGTGGGTATCAACCTACTCGTTGCTGCTTTGGGCCTCCA
GTGGGGCACTATTGTACAGGGAACCCTGCAAAGCCAGGGACAGAAATTTAACATTGGAAT
CAAAAACATGATAAATGCAGACTTCAGTGCAGCCACAGTTCTGATATCTTTTGGAGCTGT
CCTGGGAAAAACGAGCCCCACCCAAATGCTGATCATGACAATTTTAGAAATTGTTTTCTT
TGCCCACAATGAATACCTGGTTAGTGAAATATTTAAGGCCTCTGACATTGGAGCATCAAT
GACGATCCATGCCTTTGGGGCCTACTTTGGCTTGGCTGTAGCAGGCATCTTGTATCGATC
TGGACTGAGAAAGGGGCGTGAAAATGAAGAGTCCGCATACTACTCAGACTTGTTTGCAAT
GATTGGGACTCTCTTTCTGTGGATGTTTTGGCCCAGCTTTAACTCGGCCATTGCTGAACC
TGGAGACAAACAGTGCAGGGCCATTGTAAACACGTACTTCTCTCTCGCTGCCTGTGTGCT
CACAGCCTTTGCCTTCTCCAGCCTAGTGGAGCACCGAGGCAAGCTCAACATGGTTCACAT
TCAGAATGCCACCCTTGCTGGAGGAGTTGCTGTGGGCACTTGTGCGGATATGGCAATTCA
CCCATTTGGTTCTATGATTATTGGGAGCATTGCAGGAATGGTCTCTGTGCTTGGATACAA
GTTCCTGACTCCACTTTTTACTACTAAACTGAGGATCCATGATACATGTGGGGTCCATAA
CCTCCACGGCTTACCTGGTGTAGTGGGAGGCCTTGCAGGCATTGTGGCAGTAGCAATGGG
CGCCTCCAACACGTCTATGGCCATGCAGGCAGCTGCACTGGGTTCCTCTATCGGAACAGC
AGTTGTTGGAGGTCTGATGACAGGTTTAATTCTAAAGTTGCCTCTCTGGGGACAGCCATC
TGACCAGAACTGCTATGATGATTCTGTTTATTGGAAGGTCCCTAAGACGAGAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT