KRT10 ORF clone FHC09531

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09531

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Clone info.

Species

human

Clone Name

pFN21AB6122 Sequence (with vector) Restriction Map

Product ID

FHC09531

ORF Sequence

DNA sequence (1752 bp)
Protein sequence (584 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590331]

Gene

KIBB6122

Symbol

KRT10

Alias : BCIE, BIE, CK10, EHK, K10, KPP

Description

keratin 10, type I

Original Clone ID

cs00722

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1752 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 584 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11377.1 1.5e-95 99.7 KRT10 CCDS32654.1 9.7e-48 57.1 KRT9 CCDS11372.1 2.2e-40 60.2 KRT24 CCDS11400.1 7.4e-38 65.0 KRT14 CCDS11401.1 7.5e-38 60.8 KRT16 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002957 227 240 PR01248 Keratin IPR002957 248 271 PR01248 Keratin IPR002957 302 322 PR01248 Keratin IPR002957 374 389 PR01248 Keratin IPR002957 400 426 PR01248 Keratin HMMPfam IPR016044 145 458 PF00038 Filament
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000412 584 113800,148080,607602,609165 99.8 100.0 XP_005257400 624 113800,148080,607602,609165 99.7 99.8 NP_000217 623 144200,607606 57.1 100.0 NP_061889 525 607742 60.2 83.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6122 Download

>KIBB6122  1752 bp
ATGTCTGTTCGATACAGCTCAAGCAAGCACTACTCTTCCTCCCGCAGTGGAGGAGGAGGA
GGAGGAGGAGGATGTGGAGGAGGAGGAGGAGTGTCATCCCTAAGAATTTCTAGCAGCAAA
GGCTCCCTTGGTGGAGGATTTAGCTCAGGGGGGTTCAGTGGTGGCTCTTTTAGCCGTGGG
AGCTCTGGTGGGGGCTGCTTTGGGGGCTCATCAGGTGGCTATGGAGGATTAGGAGGTTTT
GGTGGAGGTAGCTTTCGTGGAAGCTATGGAAGTAGCAGCTTTGGTGGGAGTTATGGAGGC
AGCTTTGGAGGGGGCAGTTTCGGAGGTGGCAGCTTTGGTGGGGGCAGCTTTGGTGGAGGC
GGCTTTGGTGGAGGCGGCTTTGGAGGAGGCTTTGGTGGTGGATTTGGAGGAGATGGTGGC
CTTCTCTCTGGAAATGAAAAAGTAACCATGCAGAATCTGAATGACCGCCTGGCTTCCTAC
TTGGACAAAGTTCGGGCTCTGGAAGAATCAAACTATGAGCTGGAAGGCAAAATCAAGGAG
TGGTATGAAAAGCATGGCAACTCACATCAGGGGGAGCCTCGTGACTACAGCAAATACTAC
AAAACCATCGATGACCTTAAAAATCAGATTCTCAACCTAACAACTGATAATGCCAACATC
CTGCTTCAGATCGACAATGCCAGGCTGGCAGCTGATGACTTCAGGCTGAAGTATGAGAAT
GAGGTAGCTCTGCGCCAGAGCGTGGAGGCTGACATCAACGGCCTGCGTAGGGTGCTGGAT
GAGCTGACCCTGACCAAGGCTGACCTGGAGATGCAAATTGAGAGCCTGACTGAAGAGCTG
GCCTATCTGAAGAAGAACCACGAGGAGGAAATGAAAGACCTTCGAAATGTGTCCACTGGT
GATGTGAATGTGGAAATGAATGCTGCCCCGGGTGTTGATCTGACTCAACTTCTGAATAAC
ATGAGAAGCCAATATGAACAACTTGCTGAACAAAACCGCAAAGATGCTGAAGCCTGGTTC
AATGAAAAGAGCAAGGAACTGACTACAGAAATTGATAATAACATTGAACAGATATCCAGC
TATAAATCTGAGATTACTGAATTGAGACGTAATGTACAAGCTCTGGAGATAGAACTACAG
TCCCAACTGGCCTTGAAACAATCCCTGGAAGCCTCCTTGGCAGAAACAGAAGGTCGCTAC
TGTGTGCAGCTCTCACAGATTCAGGCCCAGATATCCGCTCTGGAAGAACAGTTGCAACAG
ATTCGAGCTGAAACCGAGTGCCAGAATACTGAATACCAACAACTCCTGGATATTAAGATC
CGACTGGAGAATGAAATTCAAACCTACCGCAGCCTGCTAGAAGGAGAGGGAAGTTCCGGA
GGCGGCGGACGCGGCGGCGGAAGTTTCGGCGGCGGCTACGGCGGCGGAAGCTCCGGCGGC
GGAAGCTCCGGCGGCGGCTACGGCGGCGGCCACGGCGGCAGTTCCGGCGGCGGCTACGGA
GGCGGAAGCTCCGGCGGCGGAAGCTCCGGCGGCGGCTACGGGGGCGGAAGCTCCAGCGGC
GGCCACGGCGGCAGTTCCAGCGGCGGCTACGGTGGTGGCAGTTCCGGCGGCGGCGGCGGC
GGCTACGGGGGCGGCAGCTCCGGCGGCGGCAGCAGCTCCGGCGGCGGATACGGCGGCGGC
AGCTCCAGCGGAGGCCACAAGTCCTCCTCTTCCGGGTCCGTGGGCGAGTCTTCATCTAAG
GGACCAAGATAC

ORF Protein

Cloned ORF protein sequence for pFN21AB6122 Download

>KIBB6122  584 aa
MSVRYSSSKHYSSSRSGGGGGGGGCGGGGGVSSLRISSSKGSLGGGFSSGGFSGGSFSRG
SSGGGCFGGSSGGYGGLGGFGGGSFRGSYGSSSFGGSYGGSFGGGSFGGGSFGGGSFGGG
GFGGGGFGGGFGGGFGGDGGLLSGNEKVTMQNLNDRLASYLDKVRALEESNYELEGKIKE
WYEKHGNSHQGEPRDYSKYYKTIDDLKNQILNLTTDNANILLQIDNARLAADDFRLKYEN
EVALRQSVEADINGLRRVLDELTLTKADLEMQIESLTEELAYLKKNHEEEMKDLRNVSTG
DVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSKELTTEIDNNIEQISS
YKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQ
IRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGG
GSSGGGYGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGG
GYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6122 Download

>pFN21AB6122  6457 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCTGTTCGATACAGCTCAAGCAAGCACTACTC
TTCCTCCCGCAGTGGAGGAGGAGGAGGAGGAGGAGGATGTGGAGGAGGAGGAGGAGTGTC
ATCCCTAAGAATTTCTAGCAGCAAAGGCTCCCTTGGTGGAGGATTTAGCTCAGGGGGGTT
CAGTGGTGGCTCTTTTAGCCGTGGGAGCTCTGGTGGGGGCTGCTTTGGGGGCTCATCAGG
TGGCTATGGAGGATTAGGAGGTTTTGGTGGAGGTAGCTTTCGTGGAAGCTATGGAAGTAG
CAGCTTTGGTGGGAGTTATGGAGGCAGCTTTGGAGGGGGCAGTTTCGGAGGTGGCAGCTT
TGGTGGGGGCAGCTTTGGTGGAGGCGGCTTTGGTGGAGGCGGCTTTGGAGGAGGCTTTGG
TGGTGGATTTGGAGGAGATGGTGGCCTTCTCTCTGGAAATGAAAAAGTAACCATGCAGAA
TCTGAATGACCGCCTGGCTTCCTACTTGGACAAAGTTCGGGCTCTGGAAGAATCAAACTA
TGAGCTGGAAGGCAAAATCAAGGAGTGGTATGAAAAGCATGGCAACTCACATCAGGGGGA
GCCTCGTGACTACAGCAAATACTACAAAACCATCGATGACCTTAAAAATCAGATTCTCAA
CCTAACAACTGATAATGCCAACATCCTGCTTCAGATCGACAATGCCAGGCTGGCAGCTGA
TGACTTCAGGCTGAAGTATGAGAATGAGGTAGCTCTGCGCCAGAGCGTGGAGGCTGACAT
CAACGGCCTGCGTAGGGTGCTGGATGAGCTGACCCTGACCAAGGCTGACCTGGAGATGCA
AATTGAGAGCCTGACTGAAGAGCTGGCCTATCTGAAGAAGAACCACGAGGAGGAAATGAA
AGACCTTCGAAATGTGTCCACTGGTGATGTGAATGTGGAAATGAATGCTGCCCCGGGTGT
TGATCTGACTCAACTTCTGAATAACATGAGAAGCCAATATGAACAACTTGCTGAACAAAA
CCGCAAAGATGCTGAAGCCTGGTTCAATGAAAAGAGCAAGGAACTGACTACAGAAATTGA
TAATAACATTGAACAGATATCCAGCTATAAATCTGAGATTACTGAATTGAGACGTAATGT
ACAAGCTCTGGAGATAGAACTACAGTCCCAACTGGCCTTGAAACAATCCCTGGAAGCCTC
CTTGGCAGAAACAGAAGGTCGCTACTGTGTGCAGCTCTCACAGATTCAGGCCCAGATATC
CGCTCTGGAAGAACAGTTGCAACAGATTCGAGCTGAAACCGAGTGCCAGAATACTGAATA
CCAACAACTCCTGGATATTAAGATCCGACTGGAGAATGAAATTCAAACCTACCGCAGCCT
GCTAGAAGGAGAGGGAAGTTCCGGAGGCGGCGGACGCGGCGGCGGAAGTTTCGGCGGCGG
CTACGGCGGCGGAAGCTCCGGCGGCGGAAGCTCCGGCGGCGGCTACGGCGGCGGCCACGG
CGGCAGTTCCGGCGGCGGCTACGGAGGCGGAAGCTCCGGCGGCGGAAGCTCCGGCGGCGG
CTACGGGGGCGGAAGCTCCAGCGGCGGCCACGGCGGCAGTTCCAGCGGCGGCTACGGTGG
TGGCAGTTCCGGCGGCGGCGGCGGCGGCTACGGGGGCGGCAGCTCCGGCGGCGGCAGCAG
CTCCGGCGGCGGATACGGCGGCGGCAGCTCCAGCGGAGGCCACAAGTCCTCCTCTTCCGG
GTCCGTGGGCGAGTCTTCATCTAAGGGACCAAGATACGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT