KERA ORF clone FHC09440

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09440

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Clone info.

Species

human

Clone Name

pFN21AE6340 Sequence (with vector) Restriction Map

Product ID

FHC09440

ORF Sequence

DNA sequence (1056 bp)
Protein sequence (352 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6340

Symbol

KERA

Alias : CNA2, KTN, SLRR2B

Description

keratocan

Original Clone ID

oc96340

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1056 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 352 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9037.1 2.8e-130 100.0 KERA CCDS1438.1 1.9e-65 57.3 PRELP CCDS30976.1 1.2e-40 41.9 FMOD CCDS6696.1 2.9e-40 44.0 OMD CCDS9038.1 6.4e-37 39.4 LUM The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000372 42 70 PF01462 Leucine-rich repeat-containing N-terminal IPR001611 143 165 PF00560 Leucine-rich repeat IPR001611 167 191 PF00560 Leucine-rich repeat IPR001611 283 300 PF00560 Leucine-rich repeat HMMSmart IPR000372 41 75 SM00013 Leucine-rich repeat-containing N-terminal NULL 70 89 SM00364 NULL IPR003591 71 93 SM00369 Leucine-rich repeat IPR003591 120 143 SM00369 Leucine-rich repeat NULL 120 139 SM00364 NULL NULL 141 160 SM00364 NULL IPR003591 144 164 SM00369 Leucine-rich repeat IPR003591 165 190 SM00369 Leucine-rich repeat IPR003591 191 214 SM00369 Leucine-rich repeat NULL 212 231 SM00364 NULL IPR003591 261 283 SM00369 Leucine-rich repeat NULL 261 280 SM00364 NULL ProfileScan IPR001611 72 93 PS51450 Leucine-rich repeat IPR001611 96 117 PS51450 Leucine-rich repeat IPR001611 122 142 PS51450 Leucine-rich repeat IPR001611 143 164 PS51450 Leucine-rich repeat IPR001611 167 180 PS51450 Leucine-rich repeat IPR001611 193 213 PS51450 Leucine-rich repeat IPR001611 214 235 PS51450 Leucine-rich repeat IPR001611 238 258 PS51450 Leucine-rich repeat IPR001611 263 282 PS51450 Leucine-rich repeat IPR001611 283 304 PS51450 Leucine-rich repeat IPR001611 321 342 PS51450 Leucine-rich repeat
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MAGTICFIMWVLFITDTVWSRSV 23 PRIMARY 23 2 331 EIKPPIPMALMTCFRLLQAVII 352 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_008966 352 217300,603288 100.0 100.0 NP_958505 382 601914 57.3 88.6 NP_002716 382 601914 57.3 88.6 NP_002014 376 600245 41.9 88.6 NP_002336 338 600616 39.4 88.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6340 Download

>KIEE6340  1056 bp
ATGGCAGGCACAATCTGTTTCATCATGTGGGTGTTATTCATAACAGACACTGTGTGGTCT
AGAAGTGTAAGGCAGGTCTATGAAGTACATGATTCAGATGATTGGACTATTCATGACTTC
GAGTGTCCCATGGAATGTTTCTGCCCACCCAGTTTTCCTACTGCTTTATATTGTGAAAAT
AGAGGTCTCAAAGAAATTCCTGCTATTCCTTCAAGAATTTGGTATCTTTATCTTCAAAAC
AACCTGATAGAAACCATTCCTGAAAAGCCATTTGAGAATGCCACCCAGCTAAGATGGATA
AATCTAAACAAGAACAAAATAACCAACTACGGAATTGAAAAAGGAGCCCTAAGCCAGCTG
AAGAAGTTGCTCTTCTTATTTCTGGAAGATAATGAGCTAGAGGAGGTACCTTCTCCATTG
CCAAGAAGTTTAGAACAATTACAATTAGCTAGAAATAAGGTGTCCAGAATTCCTCAAGGG
ACCTTTAGCAATCTGGAGAACCTGACCCTTCTTGACCTACAGAACAACAAATTAGTGGAC
AATGCCTTTCAAAGAGACACTTTTAAAGGACTCAAGAATCTCATGCAGCTAAACATGGCC
AAGAATGCCCTGAGGAATATGCCTCCAAGATTACCAGCCAATACAATGCAGTTGTTTTTA
GACAACAATTCCATTGAAGGAATACCAGAAAATTATTTTAATGTGATTCCTAAAGTGGCC
TTTTTGAGACTAAATCACAACAAACTGTCAGATGAGGGTCTCCCATCAAGAGGATTTGAT
GTATCATCAATTCTAGATCTTCAACTGTCGCACAATCAACTCACAAAGGTTCCCCGAATC
AGTGCTCATCTGCAGCACCTTCACCTTGATCATAACAAAATTAAAAGTGTGAATGTCTCT
GTAATATGTCCCAGCCCATCCATGCTGCCTGCAGAACGAGATTCCTTCAGTTATGGACCT
CATCTTCGCTACCTCCGTCTGGATGGAAATGAAATCAAACCACCAATTCCAATGGCTTTA
ATGACCTGCTTCAGACTTCTGCAGGCTGTCATTATT

ORF Protein

Cloned ORF protein sequence for pFN21AE6340 Download

>KIEE6340  352 aa
MAGTICFIMWVLFITDTVWSRSVRQVYEVHDSDDWTIHDFECPMECFCPPSFPTALYCEN
RGLKEIPAIPSRIWYLYLQNNLIETIPEKPFENATQLRWINLNKNKITNYGIEKGALSQL
KKLLFLFLEDNELEEVPSPLPRSLEQLQLARNKVSRIPQGTFSNLENLTLLDLQNNKLVD
NAFQRDTFKGLKNLMQLNMAKNALRNMPPRLPANTMQLFLDNNSIEGIPENYFNVIPKVA
FLRLNHNKLSDEGLPSRGFDVSSILDLQLSHNQLTKVPRISAHLQHLHLDHNKIKSVNVS
VICPSPSMLPAERDSFSYGPHLRYLRLDGNEIKPPIPMALMTCFRLLQAVII

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6340 Download

>pFN21AE6340  5761 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGCACAATCTGTTTCATCATGTGGGTGTT
ATTCATAACAGACACTGTGTGGTCTAGAAGTGTAAGGCAGGTCTATGAAGTACATGATTC
AGATGATTGGACTATTCATGACTTCGAGTGTCCCATGGAATGTTTCTGCCCACCCAGTTT
TCCTACTGCTTTATATTGTGAAAATAGAGGTCTCAAAGAAATTCCTGCTATTCCTTCAAG
AATTTGGTATCTTTATCTTCAAAACAACCTGATAGAAACCATTCCTGAAAAGCCATTTGA
GAATGCCACCCAGCTAAGATGGATAAATCTAAACAAGAACAAAATAACCAACTACGGAAT
TGAAAAAGGAGCCCTAAGCCAGCTGAAGAAGTTGCTCTTCTTATTTCTGGAAGATAATGA
GCTAGAGGAGGTACCTTCTCCATTGCCAAGAAGTTTAGAACAATTACAATTAGCTAGAAA
TAAGGTGTCCAGAATTCCTCAAGGGACCTTTAGCAATCTGGAGAACCTGACCCTTCTTGA
CCTACAGAACAACAAATTAGTGGACAATGCCTTTCAAAGAGACACTTTTAAAGGACTCAA
GAATCTCATGCAGCTAAACATGGCCAAGAATGCCCTGAGGAATATGCCTCCAAGATTACC
AGCCAATACAATGCAGTTGTTTTTAGACAACAATTCCATTGAAGGAATACCAGAAAATTA
TTTTAATGTGATTCCTAAAGTGGCCTTTTTGAGACTAAATCACAACAAACTGTCAGATGA
GGGTCTCCCATCAAGAGGATTTGATGTATCATCAATTCTAGATCTTCAACTGTCGCACAA
TCAACTCACAAAGGTTCCCCGAATCAGTGCTCATCTGCAGCACCTTCACCTTGATCATAA
CAAAATTAAAAGTGTGAATGTCTCTGTAATATGTCCCAGCCCATCCATGCTGCCTGCAGA
ACGAGATTCCTTCAGTTATGGACCTCATCTTCGCTACCTCCGTCTGGATGGAAATGAAAT
CAAACCACCAATTCCAATGGCTTTAATGACCTGCTTCAGACTTCTGCAGGCTGTCATTAT
TGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T