SENP5 ORF clone FHC09347

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09347

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Clone info.

Species

human

Clone Name

pFN21AE6716 Sequence (with vector) Restriction Map

Product ID

FHC09347

ORF Sequence

DNA sequence (2265 bp)
Protein sequence (755 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6716

Symbol

SENP5

Description

SUMO1/sentrin specific peptidase 5, transcript variant 1

Original Clone ID

oc96716

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2265 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 755 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3322.1 0 99.9 SENP5 CCDS77876.1 0 93.8 SENP5 CCDS73958.1 1.4e-57 53.5 SENP3 CCDS44868.2 7.7e-27 29.8 SENP1 CCDS33902.1 1.3e-23 30.6 SENP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003653 581 752 PF02902 Peptidase C48 ProfileScan IPR003653 567 724 PS50600 Peptidase C48
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_689912 755 612845 99.9 100.0 XP_011510846 755 612845 99.9 100.0 XP_005269367 727 612845 96.2 100.0 XP_011510847 643 612845 99.8 83.3 XP_005269369 681 612845 90.1 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6716 Download

>KIEE6716  2265 bp
ATGAAAAAACAGAGGAAAATTCTATGGAGGAAAGGAATCCACTTAGCCTTTTCTGAGAAA
TGGAATACTGGGTTTGGAGGCTTTAAGAAGTTTTATTTTCACCAACACTTGTGCATTCTG
AAAGCTAAGCTGGGAAGGCCAGTTACTTGGAATAGACAGTTGAGACATTTCCAGGGTAGA
AAGAAAGCTCTTCAAATCCAGAAAACGTGGATCAAGGATGAACACCTTTGTGCTAAGACC
AAGTTCAATGTGGCTACTCAAAATGTTAGTACTTTGTCCTCTAAAGTGAAAAGAAAGGAC
GCTAAACACTTCATTTCCTCCTCAAAGACTCTCCTGAGACTCCAAGCAGAGAAGCTGTTG
TCATCAGCAAAGAATTCTGACCATGAATACTGCAGAGAGAAAAATCTCTTGAAGGCAGTT
ACTGACTTTCCATCAAATAGTGCTTTAGGTCAGGCCAATGGTCACAGACCTAGGACAGAC
CCACAACCTTCTGACTTTCCCATGAAGTTCAATGGGGAGAGCCAAAGTCCAGGTGAGAGT
GGCACGATTGTGGTCACCTTGAACAACCATAAGAGAAAGGGCTTTTGTTACGGCTGCTGC
CAAGGGCCGGAGCACCACAGGAATGGGGGACCCTTGATTCCAAAAAAGTTCCAACTTAAC
CAACATAGAAGGATAAAATTATCTCCTCTTATGATGTATGAGAAATTATCCATGATTAGA
TTTCGGTACAGGATTCTCAGATCCCAGCACTTCAGAACCAAAAGCAAGGTTTGCAAGCTA
AGAAAAGCCCAGCGAAGCTGGGTACAGAAAGTCACTGGGGACCATCAAGAGACCCGTAGG
GAGAACGGTGAGGGTGGCAGTTGCAGCCCATTTCCTTCCCCAGAACCTAAAGACCCTTCT
TGTCGGCATCAGCCGTACTTTCCAGATATGGACAGCAGTGCTGTGGTGAAGGGGACGAAC
TCTCATGTGCCTGATTGCCACACTAAAGGAAGCTCTTTCTTGGGCAAGGAGCTTAGTTTA
GACGAAGCATTCCCTGACCAACAGAATGGCAGTGCCACAAACGCCTGGGACCAGTCATCC
TGTTCTTCTCCTAAGTGGGAGTGTACAGAGCTGATTCATGACATCCCCTTACCAGAACAT
CGTTCTAATACCATGTTCATTTCAGAAACTGAAAGAGAAATTATGACTCTGGGTCAGGAA
AATCAGACAAGTTCTGTCAGTGATGACAGAGTAAAACTGTCAGTGTCTGGAGCAGATACA
TCTGTGAGTAGCGTAGATGGGCCTGTGTCCCAAAAGGCTGTTCAAAATGAGAACTCATAC
CAGATGGAGGAGGATGGATCTCTCAAGCAGAGCATTCTTAGTTCTGAGTTGCTGGACCAC
CCTTACTGTAAAAGTCCACTGGAGGCTCCCTTGGTGTGCAGTGGACTCAAACTAGAAAAT
CAAGTAGGAGGTGGAAAGAACAGTCAGAAAGCCTCTCCAGTGGATGATGAACAGCTGTCA
GTCTGTCTTTCTGGATTCCTAGATGAGGTTATGAAGAAGTATGGCAGTTTGGTTCCACTC
AGTGAAAAAGAAGTCCTTGGAAGATTAAAAGATGTCTTTAATGAAGACTTTTCTAATAGA
AAACCATTTATCAATAGGGAAATAACAAACTATCGGGCCAGACATCAAAAATGTAACTTC
CGTATCTTCTATAATAAACACATGCTGGATATGGACGACCTGGCGACTCTGGATGGTCAG
AACTGGCTGAATGACCAGGTCATTAATATGTATGGTGAGCTGATAATGGATGCAGTCCCA
GACAAAGTTCACTTCTTCAACAGCTTTTTTCATAGACAGCTGGTAACCAAAGGATATAAT
GGAGTAAAAAGATGGACTAAAAAGGTGGATTTGTTTAAAAAGAGTCTTCTGTTGATTCCT
ATTCACCTGGAAGTCCACTGGTCTCTCATTACTGTGACACTCTCTAATCGAATTATTTCA
TTTTATGATTCCCAAGGCATTCATTTTAAGTTTTGTGTAGAGAATATAAGAAAGTATTTG
CTGACTGAAGCCAGAGAAAAAAATAGACCTGAATTTCTTCAGGGTTGGCAGACTGCTGTT
ACGAAGTGTATTCCACAACAGAAAAACGACAGTGACTGTGGAGTCTTTGTGCTCCAGTAC
TGCAAGTGCCTCGCCTTAGAGCAGCCTTTCCAGTTTTCACAAGAAGACATGCCCCGAGTG
CGGAAGAGGATTTACAAGGAGCTATGTGAGTGCCGGCTCATGGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE6716 Download

>KIEE6716  755 aa
MKKQRKILWRKGIHLAFSEKWNTGFGGFKKFYFHQHLCILKAKLGRPVTWNRQLRHFQGR
KKALQIQKTWIKDEHLCAKTKFNVATQNVSTLSSKVKRKDAKHFISSSKTLLRLQAEKLL
SSAKNSDHEYCREKNLLKAVTDFPSNSALGQANGHRPRTDPQPSDFPMKFNGESQSPGES
GTIVVTLNNHKRKGFCYGCCQGPEHHRNGGPLIPKKFQLNQHRRIKLSPLMMYEKLSMIR
FRYRILRSQHFRTKSKVCKLRKAQRSWVQKVTGDHQETRRENGEGGSCSPFPSPEPKDPS
CRHQPYFPDMDSSAVVKGTNSHVPDCHTKGSSFLGKELSLDEAFPDQQNGSATNAWDQSS
CSSPKWECTELIHDIPLPEHRSNTMFISETEREIMTLGQENQTSSVSDDRVKLSVSGADT
SVSSVDGPVSQKAVQNENSYQMEEDGSLKQSILSSELLDHPYCKSPLEAPLVCSGLKLEN
QVGGGKNSQKASPVDDEQLSVCLSGFLDEVMKKYGSLVPLSEKEVLGRLKDVFNEDFSNR
KPFINREITNYRARHQKCNFRIFYNKHMLDMDDLATLDGQNWLNDQVINMYGELIMDAVP
DKVHFFNSFFHRQLVTKGYNGVKRWTKKVDLFKKSLLLIPIHLEVHWSLITVTLSNRIIS
FYDSQGIHFKFCVENIRKYLLTEAREKNRPEFLQGWQTAVTKCIPQQKNDSDCGVFVLQY
CKCLALEQPFQFSQEDMPRVRKRIYKELCECRLMD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6716 Download

>pFN21AE6716  6970 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAAAAACAGAGGAAAATTCTATGGAGGAAAGG
AATCCACTTAGCCTTTTCTGAGAAATGGAATACTGGGTTTGGAGGCTTTAAGAAGTTTTA
TTTTCACCAACACTTGTGCATTCTGAAAGCTAAGCTGGGAAGGCCAGTTACTTGGAATAG
ACAGTTGAGACATTTCCAGGGTAGAAAGAAAGCTCTTCAAATCCAGAAAACGTGGATCAA
GGATGAACACCTTTGTGCTAAGACCAAGTTCAATGTGGCTACTCAAAATGTTAGTACTTT
GTCCTCTAAAGTGAAAAGAAAGGACGCTAAACACTTCATTTCCTCCTCAAAGACTCTCCT
GAGACTCCAAGCAGAGAAGCTGTTGTCATCAGCAAAGAATTCTGACCATGAATACTGCAG
AGAGAAAAATCTCTTGAAGGCAGTTACTGACTTTCCATCAAATAGTGCTTTAGGTCAGGC
CAATGGTCACAGACCTAGGACAGACCCACAACCTTCTGACTTTCCCATGAAGTTCAATGG
GGAGAGCCAAAGTCCAGGTGAGAGTGGCACGATTGTGGTCACCTTGAACAACCATAAGAG
AAAGGGCTTTTGTTACGGCTGCTGCCAAGGGCCGGAGCACCACAGGAATGGGGGACCCTT
GATTCCAAAAAAGTTCCAACTTAACCAACATAGAAGGATAAAATTATCTCCTCTTATGAT
GTATGAGAAATTATCCATGATTAGATTTCGGTACAGGATTCTCAGATCCCAGCACTTCAG
AACCAAAAGCAAGGTTTGCAAGCTAAGAAAAGCCCAGCGAAGCTGGGTACAGAAAGTCAC
TGGGGACCATCAAGAGACCCGTAGGGAGAACGGTGAGGGTGGCAGTTGCAGCCCATTTCC
TTCCCCAGAACCTAAAGACCCTTCTTGTCGGCATCAGCCGTACTTTCCAGATATGGACAG
CAGTGCTGTGGTGAAGGGGACGAACTCTCATGTGCCTGATTGCCACACTAAAGGAAGCTC
TTTCTTGGGCAAGGAGCTTAGTTTAGACGAAGCATTCCCTGACCAACAGAATGGCAGTGC
CACAAACGCCTGGGACCAGTCATCCTGTTCTTCTCCTAAGTGGGAGTGTACAGAGCTGAT
TCATGACATCCCCTTACCAGAACATCGTTCTAATACCATGTTCATTTCAGAAACTGAAAG
AGAAATTATGACTCTGGGTCAGGAAAATCAGACAAGTTCTGTCAGTGATGACAGAGTAAA
ACTGTCAGTGTCTGGAGCAGATACATCTGTGAGTAGCGTAGATGGGCCTGTGTCCCAAAA
GGCTGTTCAAAATGAGAACTCATACCAGATGGAGGAGGATGGATCTCTCAAGCAGAGCAT
TCTTAGTTCTGAGTTGCTGGACCACCCTTACTGTAAAAGTCCACTGGAGGCTCCCTTGGT
GTGCAGTGGACTCAAACTAGAAAATCAAGTAGGAGGTGGAAAGAACAGTCAGAAAGCCTC
TCCAGTGGATGATGAACAGCTGTCAGTCTGTCTTTCTGGATTCCTAGATGAGGTTATGAA
GAAGTATGGCAGTTTGGTTCCACTCAGTGAAAAAGAAGTCCTTGGAAGATTAAAAGATGT
CTTTAATGAAGACTTTTCTAATAGAAAACCATTTATCAATAGGGAAATAACAAACTATCG
GGCCAGACATCAAAAATGTAACTTCCGTATCTTCTATAATAAACACATGCTGGATATGGA
CGACCTGGCGACTCTGGATGGTCAGAACTGGCTGAATGACCAGGTCATTAATATGTATGG
TGAGCTGATAATGGATGCAGTCCCAGACAAAGTTCACTTCTTCAACAGCTTTTTTCATAG
ACAGCTGGTAACCAAAGGATATAATGGAGTAAAAAGATGGACTAAAAAGGTGGATTTGTT
TAAAAAGAGTCTTCTGTTGATTCCTATTCACCTGGAAGTCCACTGGTCTCTCATTACTGT
GACACTCTCTAATCGAATTATTTCATTTTATGATTCCCAAGGCATTCATTTTAAGTTTTG
TGTAGAGAATATAAGAAAGTATTTGCTGACTGAAGCCAGAGAAAAAAATAGACCTGAATT
TCTTCAGGGTTGGCAGACTGCTGTTACGAAGTGTATTCCACAACAGAAAAACGACAGTGA
CTGTGGAGTCTTTGTGCTCCAGTACTGCAAGTGCCTCGCCTTAGAGCAGCCTTTCCAGTT
TTCACAAGAAGACATGCCCCGAGTGCGGAAGAGGATTTACAAGGAGCTATGTGAGTGCCG
GCTCATGGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT