PDCD4 ORF clone FHC09187

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09187

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Clone info.

Species

human

Clone Name

pFN21AE1311 Sequence (with vector) Restriction Map

Product ID

FHC09187

ORF Sequence

DNA sequence (1407 bp)
Protein sequence (469 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590295]

Gene

KIEE1311

Symbol

PDCD4

Alias : H731

Description

programmed cell death 4 (neoplastic transformation inhibitor), transcript variant 1

Original Clone ID

oc01311

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1407 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 469 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7567.1 4e-169 99.6 PDCD4 CCDS44478.1 1.5e-163 99.6 PDCD4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003891 164 274 PF02847 Initiation factor eIF-4 gamma IPR003891 329 439 PF02847 Initiation factor eIF-4 gamma HMMSmart IPR003891 164 275 SM00544 Initiation factor eIF-4 gamma IPR003891 327 440 SM00544 Initiation factor eIF-4 gamma ProfileScan IPR003891 163 284 PS51366 Initiation factor eIF-4 gamma IPR003891 326 449 PS51366 Initiation factor eIF-4 gamma
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055271 469 608610 99.6 100.0 NP_663314 458 608610 99.6 96.8 NP_001186421 455 608610 96.6 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1311 Download

>KIEE1311  1407 bp
ATGGATGTAGAAAATGAGCAGATACTGAATGTAAACCCTGCAGATCCTGATAACTTAAGT
GACTCTCTCTTTTCCGGTGATGAAGAAAATGCTGGGACTGAGGAAGTAAAGAATGAAATA
AATGGAAATTGGATTTCAGCATACTCCATTAACGAAGCTAGAATTAATGCCAAGGCAAAA
AGGCGACTAAGGAAAAACTCATCCCGGGACTCTGGCAGAGGCGATTCGGTCAGCGACAGT
GGGAGTGACGCCCTTAGAAGTGGATTAACTGTGCCAACCAGTCCAAAGGGAAGGTTGCTG
GATAGGCGATCCAGATCTGGGAAAGGAAGGGGACTACCAAAGAAAGGTGGTGCAGGAGGC
AAAGGTGTCTGGGGTACACCTGGACAGGTGTATGATGTGGAGGAGGTGGATGTGAAAGAT
CCTAACTATGATGATGACCAGGAGAACTGTGTTTATGAAACTGTAGTTTTGCCTTTGGAT
GAAAGGGCATTTGAGAAGACTTTAACACCAATCATACAGGAATATTTTGAGCATGGAGAT
ACTAATGAAGTTGCGGAAATGTTAAGAGATTTAAATCTTGGTGAAATGAAAAGTGGAGTA
CCAGTGTTGGCAGTATCCTTAGCATTGGAGGGGAAGGCTAGTCATAGAGAGATGACATCT
AAGCTTCTTTCTGACCTTTGTGGGACAGTAATGAGCACAACTGATGTGGAAAAATCATTT
GATAAATTGTTGAAAGATCTACCTGAATTAGCACTGGATACTCCTAGAGCACCACAGTTG
GTGGGCCAGTTTATTGCTAGAGCTGTTGGAGATGGAATTTTATGTAATACCTATATTGAT
AGTTACAAAGGAACTGTAGATTGTGTGCAGGCTAGAGCTGCTCTGGATAAGGCTACCGTG
CTTCTGAGTATGTCTAAAGGTGGAAAGCGTAAAGATAGTGTGTGGGGCTCTGGAGGTGGG
CAGCAATCTGTCAATCACCTTGTTAAAGAGATTGATATGCTGCTGAAAGAATATTTACTC
TCTGGAGACATATCTGAAGCTGAACATTGCCTTAAGGAACTGGAAGTACCTCATTTTCAC
CATGAGCTTGTATATGAAGCTATTATAATGGTTTTAGAGTCAACTGGAGAAAGTACATTT
AAGATGATTTTGGATTTATTAAAGTCCCTTTGGAAGTCTTCTACCATTACTGTAGACCAA
ATGAAAAGAGGTTATGAGAGAATTTACAATGAAATTCCGGACATTAATCTGGATGTCCCA
CATTCATACTCTGTGCTGGAGCGGTTTGTAGAAGAATGTTTTCAGGCTGGAATAATTTCC
AAACAACTCAGAGATCTTTGTCCTTCAAGGGGCAGAAAGCGTTTTGTAAGCGAAGGAGAT
GGAGGTCGTCTTAAACCAGAGAGCTAC

ORF Protein

Cloned ORF protein sequence for pFN21AE1311 Download

>KIEE1311  469 aa
MDVENEQILNVNPADPDNLSDSLFSGDEENAGTEEVKNEINGNWISAYSINEARINAKAK
RRLRKNSSRDSGRGDSVSDSGSDALRSGLTVPTSPKGRLLDRRSRSGKGRGLPKKGGAGG
KGVWGTPGQVYDVEEVDVKDPNYDDDQENCVYETVVLPLDERAFEKTLTPIIQEYFEHGD
TNEVAEMLRDLNLGEMKSGVPVLAVSLALEGKASHREMTSKLLSDLCGTVMSTTDVEKSF
DKLLKDLPELALDTPRAPQLVGQFIARAVGDGILCNTYIDSYKGTVDCVQARAALDKATV
LLSMSKGGKRKDSVWGSGGGQQSVNHLVKEIDMLLKEYLLSGDISEAEHCLKELEVPHFH
HELVYEAIIMVLESTGESTFKMILDLLKSLWKSSTITVDQMKRGYERIYNEIPDINLDVP
HSYSVLERFVEECFQAGIISKQLRDLCPSRGRKRFVSEGDGGRLKPESY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1311 Download

>pFN21AE1311  6112 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATGTAGAAAATGAGCAGATACTGAATGTAAA
CCCTGCAGATCCTGATAACTTAAGTGACTCTCTCTTTTCCGGTGATGAAGAAAATGCTGG
GACTGAGGAAGTAAAGAATGAAATAAATGGAAATTGGATTTCAGCATACTCCATTAACGA
AGCTAGAATTAATGCCAAGGCAAAAAGGCGACTAAGGAAAAACTCATCCCGGGACTCTGG
CAGAGGCGATTCGGTCAGCGACAGTGGGAGTGACGCCCTTAGAAGTGGATTAACTGTGCC
AACCAGTCCAAAGGGAAGGTTGCTGGATAGGCGATCCAGATCTGGGAAAGGAAGGGGACT
ACCAAAGAAAGGTGGTGCAGGAGGCAAAGGTGTCTGGGGTACACCTGGACAGGTGTATGA
TGTGGAGGAGGTGGATGTGAAAGATCCTAACTATGATGATGACCAGGAGAACTGTGTTTA
TGAAACTGTAGTTTTGCCTTTGGATGAAAGGGCATTTGAGAAGACTTTAACACCAATCAT
ACAGGAATATTTTGAGCATGGAGATACTAATGAAGTTGCGGAAATGTTAAGAGATTTAAA
TCTTGGTGAAATGAAAAGTGGAGTACCAGTGTTGGCAGTATCCTTAGCATTGGAGGGGAA
GGCTAGTCATAGAGAGATGACATCTAAGCTTCTTTCTGACCTTTGTGGGACAGTAATGAG
CACAACTGATGTGGAAAAATCATTTGATAAATTGTTGAAAGATCTACCTGAATTAGCACT
GGATACTCCTAGAGCACCACAGTTGGTGGGCCAGTTTATTGCTAGAGCTGTTGGAGATGG
AATTTTATGTAATACCTATATTGATAGTTACAAAGGAACTGTAGATTGTGTGCAGGCTAG
AGCTGCTCTGGATAAGGCTACCGTGCTTCTGAGTATGTCTAAAGGTGGAAAGCGTAAAGA
TAGTGTGTGGGGCTCTGGAGGTGGGCAGCAATCTGTCAATCACCTTGTTAAAGAGATTGA
TATGCTGCTGAAAGAATATTTACTCTCTGGAGACATATCTGAAGCTGAACATTGCCTTAA
GGAACTGGAAGTACCTCATTTTCACCATGAGCTTGTATATGAAGCTATTATAATGGTTTT
AGAGTCAACTGGAGAAAGTACATTTAAGATGATTTTGGATTTATTAAAGTCCCTTTGGAA
GTCTTCTACCATTACTGTAGACCAAATGAAAAGAGGTTATGAGAGAATTTACAATGAAAT
TCCGGACATTAATCTGGATGTCCCACATTCATACTCTGTGCTGGAGCGGTTTGTAGAAGA
ATGTTTTCAGGCTGGAATAATTTCCAAACAACTCAGAGATCTTTGTCCTTCAAGGGGCAG
AAAGCGTTTTGTAAGCGAAGGAGATGGAGGTCGTCTTAAACCAGAGAGCTACGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT