SLC6A13 ORF clone FHC09098

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09098

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Clone info.

Species

human

Clone Name

pFN21AE6412 Sequence (with vector) Restriction Map

Product ID

FHC09098

ORF Sequence

DNA sequence (1806 bp)
Protein sequence (602 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6412

Symbol

SLC6A13

Alias : GAT-2, GAT2, GAT3

Description

solute carrier family 6 (neurotransmitter transporter), member 13, transcript variant 1

Original Clone ID

oc96412

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1806 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 602 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8502.1 0 100.0 SLC6A13 CCDS53729.1 2.5e-196 84.7 SLC6A13 CCDS8501.1 4.2e-190 71.5 SLC6A12 CCDS2602.1 6.2e-188 70.8 SLC6A11 CCDS33705.1 2.3e-164 63.2 SLC6A6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000175 40 61 PR00176 Sodium:neurotransmitter symporter IPR000175 69 88 PR00176 Sodium:neurotransmitter symporter IPR000175 113 139 PR00176 Sodium:neurotransmitter symporter IPR002981 168 178 PR01196 Sodium:neurotransmitter symporter IPR000175 233 250 PR00176 Sodium:neurotransmitter symporter IPR000175 315 335 PR00176 Sodium:neurotransmitter symporter IPR000175 369 388 PR00176 Sodium:neurotransmitter symporter IPR000175 453 473 PR00176 Sodium:neurotransmitter symporter IPR000175 493 513 PR00176 Sodium:neurotransmitter symporter IPR002981 559 572 PR01196 Sodium:neurotransmitter symporter IPR002981 573 583 PR01196 Sodium:neurotransmitter symporter IPR002981 585 602 PR01196 Sodium:neurotransmitter symporter HMMPfam IPR000175 32 553 PF00209 Sodium:neurotransmitter symporter ProfileScan IPR000175 31 558 PS50267 Sodium:neurotransmitter symporter
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 37 NKMEFVLSVAGEIIGLGNVWRFP 59 SECONDARY 23 2 69 AFFIPYLVFLFTCGIPVFLLETA 91 PRIMARY 23 3 117 ASQMIVILLNVYYIIVLAWALFY 139 PRIMARY 23 4 206 WELALCLLLAWVICYFCIWKGVK 228 PRIMARY 23 5 234 VYFTATFPYLMLVVLLIRGVTLP 256 PRIMARY 23 6 281 DAGTQIFFSFAICLGCLTALGS 302 PRIMARY 22 7 315 IALCFLNSGTSFVAGFAIFSILG 337 SECONDARY 23 8 384 VLLGLDSQFVCVESLVTALVDMY 406 PRIMARY 23 9 419 LILGVSVVSFLVGLIMLTEGGMY 441 PRIMARY 23 10 451 ASGMCLLFVAIFESLCVAWVYGA 473 PRIMARY 23 11 491 LIKYCWLFLTPAVCTATFLFSLI 513 SECONDARY 23 12 529 WGDALGWLLALSSMVCIPAWSLY 551 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057699 602 615097 100.0 100.0 XP_011519314 483 615097 100.0 80.2 NP_001177926 510 615097 84.7 100.0 NP_003035 614 603080 71.5 96.5 NP_001116319 614 603080 71.5 96.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6412 Download

>KIEE6412  1806 bp
ATGGATAGCAGGGTCTCAGGCACAACCAGTAATGGAGAGACAAAACCAGTGTATCCAGTC
ATGGAAAAGAAGGAGGAAGATGGCACCCTGGAGCGGGGGCACTGGAACAACAAGATGGAG
TTTGTGCTGTCAGTGGCTGGGGAGATCATTGGCTTAGGCAACGTCTGGAGGTTTCCCTAT
CTCTGCTACAAAAATGGGGGAGGTGCCTTCTTCATCCCCTACCTCGTCTTCCTCTTTACC
TGTGGCATTCCTGTCTTCCTTCTGGAGACAGCACTAGGCCAGTACACTAGCCAGGGAGGC
GTCACAGCCTGGAGGAAGATCTGCCCCATCTTTGAGGGCATTGGCTATGCCTCCCAGATG
ATCGTCATCCTCCTCAACGTCTACTACATCATTGTGTTGGCCTGGGCCCTGTTCTACCTC
TTCAGCAGCTTCACCATCGACCTGCCCTGGGGCGGCTGCTACCATGAGTGGAACACAGAA
CACTGTATGGAGTTCCAGAAGACCAACGGCTCCCTGAATGGTACCTCTGAGAATGCCACC
TCTCCTGTCATCGAGTTCTGGGAGCGGCGGGTCTTGAAGATCTCTGATGGGATCCAGCAC
CTGGGGGCCCTGCGCTGGGAGCTGGCTCTGTGCCTCCTGCTGGCCTGGGTCATCTGCTAC
TTCTGCATCTGGAAGGGGGTGAAGTCCACAGGCAAGGTGGTGTACTTCACGGCCACATTT
CCTTACCTCATGCTGGTGGTCCTGTTAATTCGAGGGGTGACGTTGCCTGGGGCAGCCCAA
GGAATTCAGTTTTACCTGTACCCAAACCTCACGCGTCTGTGGGATCCCCAGGTGTGGATG
GATGCAGGCACCCAGATATTCTTCTCCTTCGCCATCTGTCTTGGGTGCCTGACAGCCCTG
GGCAGCTACAACAAGTACCACAACAACTGCTACAGGGACTGCATCGCCCTCTGCTTCCTC
AACAGCGGCACCAGCTTTGTGGCCGGCTTTGCCATCTTCTCCATCCTGGGCTTCATGTCT
CAGGAGCAGGGGGTGCCCATTTCTGAGGTGGCCGAGTCAGGCCCTGGCCTGGCTTTCATC
GCTTACCCGCGGGCTGTGGTGATGCTGCCCTTCTCTCCTCTCTGGGCCTGCTGTTTCTTC
TTCATGGTCGTTCTCCTGGGACTGGATAGCCAGTTTGTGTGTGTAGAAAGCCTGGTGACA
GCGCTGGTGGACATGTACCCTCACGTGTTCCGCAAGAAGAACCGGAGGGAAGTCCTCATC
CTTGGAGTATCTGTCGTCTCCTTCCTTGTGGGGCTGATCATGCTCACAGAGGGCGGAATG
TACGTGTTCCAGCTCTTTGACTACTATGCGGCCAGTGGCATGTGCCTCCTGTTCGTGGCC
ATCTTCGAGTCCCTCTGTGTGGCTTGGGTTTACGGAGCCAAGCGCTTCTACGACAACATC
GAAGACATGATTGGGTACAGGCCATGGCCTCTTATCAAATACTGTTGGCTCTTCCTCACA
CCAGCTGTGTGCACAGCCACCTTTCTCTTCTCCCTGATAAAGTACACTCCGCTGACCTAC
AACAAGAAGTACACGTACCCGTGGTGGGGCGATGCCCTGGGCTGGCTCCTGGCTCTGTCC
TCCATGGTCTGCATTCCTGCCTGGAGCCTCTACAGACTCGGAACCCTCAAGGGCCCCTTC
AGAGAGAGAATCCGTCAGCTCATGTGCCCAGCCGAGGACTTGCCCCAGCGGAACCCAGCA
GGACCCTCGGCTCCCGCCACCCCCAGGACCTCACTGCTCAGACTCACAGAGCTAGAGTCT
CACTGC

ORF Protein

Cloned ORF protein sequence for pFN21AE6412 Download

>KIEE6412  602 aa
MDSRVSGTTSNGETKPVYPVMEKKEEDGTLERGHWNNKMEFVLSVAGEIIGLGNVWRFPY
LCYKNGGGAFFIPYLVFLFTCGIPVFLLETALGQYTSQGGVTAWRKICPIFEGIGYASQM
IVILLNVYYIIVLAWALFYLFSSFTIDLPWGGCYHEWNTEHCMEFQKTNGSLNGTSENAT
SPVIEFWERRVLKISDGIQHLGALRWELALCLLLAWVICYFCIWKGVKSTGKVVYFTATF
PYLMLVVLLIRGVTLPGAAQGIQFYLYPNLTRLWDPQVWMDAGTQIFFSFAICLGCLTAL
GSYNKYHNNCYRDCIALCFLNSGTSFVAGFAIFSILGFMSQEQGVPISEVAESGPGLAFI
AYPRAVVMLPFSPLWACCFFFMVVLLGLDSQFVCVESLVTALVDMYPHVFRKKNRREVLI
LGVSVVSFLVGLIMLTEGGMYVFQLFDYYAASGMCLLFVAIFESLCVAWVYGAKRFYDNI
EDMIGYRPWPLIKYCWLFLTPAVCTATFLFSLIKYTPLTYNKKYTYPWWGDALGWLLALS
SMVCIPAWSLYRLGTLKGPFRERIRQLMCPAEDLPQRNPAGPSAPATPRTSLLRLTELES
HC

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6412 Download

>pFN21AE6412  6511 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATAGCAGGGTCTCAGGCACAACCAGTAATGG
AGAGACAAAACCAGTGTATCCAGTCATGGAAAAGAAGGAGGAAGATGGCACCCTGGAGCG
GGGGCACTGGAACAACAAGATGGAGTTTGTGCTGTCAGTGGCTGGGGAGATCATTGGCTT
AGGCAACGTCTGGAGGTTTCCCTATCTCTGCTACAAAAATGGGGGAGGTGCCTTCTTCAT
CCCCTACCTCGTCTTCCTCTTTACCTGTGGCATTCCTGTCTTCCTTCTGGAGACAGCACT
AGGCCAGTACACTAGCCAGGGAGGCGTCACAGCCTGGAGGAAGATCTGCCCCATCTTTGA
GGGCATTGGCTATGCCTCCCAGATGATCGTCATCCTCCTCAACGTCTACTACATCATTGT
GTTGGCCTGGGCCCTGTTCTACCTCTTCAGCAGCTTCACCATCGACCTGCCCTGGGGCGG
CTGCTACCATGAGTGGAACACAGAACACTGTATGGAGTTCCAGAAGACCAACGGCTCCCT
GAATGGTACCTCTGAGAATGCCACCTCTCCTGTCATCGAGTTCTGGGAGCGGCGGGTCTT
GAAGATCTCTGATGGGATCCAGCACCTGGGGGCCCTGCGCTGGGAGCTGGCTCTGTGCCT
CCTGCTGGCCTGGGTCATCTGCTACTTCTGCATCTGGAAGGGGGTGAAGTCCACAGGCAA
GGTGGTGTACTTCACGGCCACATTTCCTTACCTCATGCTGGTGGTCCTGTTAATTCGAGG
GGTGACGTTGCCTGGGGCAGCCCAAGGAATTCAGTTTTACCTGTACCCAAACCTCACGCG
TCTGTGGGATCCCCAGGTGTGGATGGATGCAGGCACCCAGATATTCTTCTCCTTCGCCAT
CTGTCTTGGGTGCCTGACAGCCCTGGGCAGCTACAACAAGTACCACAACAACTGCTACAG
GGACTGCATCGCCCTCTGCTTCCTCAACAGCGGCACCAGCTTTGTGGCCGGCTTTGCCAT
CTTCTCCATCCTGGGCTTCATGTCTCAGGAGCAGGGGGTGCCCATTTCTGAGGTGGCCGA
GTCAGGCCCTGGCCTGGCTTTCATCGCTTACCCGCGGGCTGTGGTGATGCTGCCCTTCTC
TCCTCTCTGGGCCTGCTGTTTCTTCTTCATGGTCGTTCTCCTGGGACTGGATAGCCAGTT
TGTGTGTGTAGAAAGCCTGGTGACAGCGCTGGTGGACATGTACCCTCACGTGTTCCGCAA
GAAGAACCGGAGGGAAGTCCTCATCCTTGGAGTATCTGTCGTCTCCTTCCTTGTGGGGCT
GATCATGCTCACAGAGGGCGGAATGTACGTGTTCCAGCTCTTTGACTACTATGCGGCCAG
TGGCATGTGCCTCCTGTTCGTGGCCATCTTCGAGTCCCTCTGTGTGGCTTGGGTTTACGG
AGCCAAGCGCTTCTACGACAACATCGAAGACATGATTGGGTACAGGCCATGGCCTCTTAT
CAAATACTGTTGGCTCTTCCTCACACCAGCTGTGTGCACAGCCACCTTTCTCTTCTCCCT
GATAAAGTACACTCCGCTGACCTACAACAAGAAGTACACGTACCCGTGGTGGGGCGATGC
CCTGGGCTGGCTCCTGGCTCTGTCCTCCATGGTCTGCATTCCTGCCTGGAGCCTCTACAG
ACTCGGAACCCTCAAGGGCCCCTTCAGAGAGAGAATCCGTCAGCTCATGTGCCCAGCCGA
GGACTTGCCCCAGCGGAACCCAGCAGGACCCTCGGCTCCCGCCACCCCCAGGACCTCACT
GCTCAGACTCACAGAGCTAGAGTCTCACTGCGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT