NOSTRIN ORF clone FHC08729

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC08729

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Clone info.

Species

human

Clone Name

pFN21AE1450 Sequence (with vector) Restriction Map

Product ID

FHC08729

ORF Sequence

DNA sequence (1284 bp)
Protein sequence (428 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590237]

Gene

KIEE1450

Symbol

NOSTRIN

Alias : DaIP2

Description

nitric oxide synthase trafficking, transcript variant 1

Original Clone ID

oc01450

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1284 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 428 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS42772.1 1.9e-99 99.8 NOSTRIN CCDS54416.1 2e-99 99.8 NOSTRIN CCDS42771.1 2.1e-99 99.8 NOSTRIN CCDS54415.1 1.6e-68 87.4 NOSTRIN The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001452 363 373 PR00452 Src homology-3 domain IPR001452 377 392 PR00452 Src homology-3 domain IPR001452 394 403 PR00452 Src homology-3 domain IPR001452 405 417 PR00452 Src homology-3 domain HMMPfam IPR001452 366 411 PF00018 Src homology-3 domain HMMSmart IPR001452 363 418 SM00326 Src homology-3 domain ProfileScan IPR001452 360 419 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_443178 428 607496 99.8 100.0 NP_001165103 478 607496 99.8 100.0 NP_001034813 506 607496 99.8 100.0 XP_016858767 485 607496 100.0 89.5 XP_016858768 485 607496 87.4 94.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1450 Download

>KIEE1450  1284 bp
ATGAAATCCACAGCGGACCTGCATCAAAAACTTGGCAAAGCAATTGAATTGGAAGCAATA
AAACCGACTTATCAAGTCCTAAATGTACAAGAGAAGAAGAGAAAATCACTTGACAATGAA
GTTGAAAAGACAGCAAATCTTGTCATTAGCAACTGGAATCAGCAAATTAAGGCCAAGAAG
AAATTAATGGTTAGTACCAAGAAACATGAAGCACTTTTCCAGCTTGTAGAAAGCTCCAAG
CAATCTATGACTGAGAAGGAGAAGCGGAAGCTCCTCAATAAACTGACAAAATCAACTGAA
AAGTTGGAAAAGGAAGATGAAAATTACTACCAAAAAAACATGGCGGGTTATTCTACCAGA
CTGAAATGGGAAAACACACTAGAGAACTGCTACCAGAGCATTCTGGAGCTGGAGAAGGAA
AGAATTCAACTTTTATGCAATAACTTAAACCAGTACAGCCAACATATTTCTCTTTTTGGC
CAAACCCTGACCACATGCCACACGCAGATTCACTGTGCCATCAGCAAGATTGACATTGAA
AAAGATATCCAGGCTGTAATGGAAGAAACTGCAATTTTATCTACAGAAAACAAATCTGAG
TTCCTGTTAACGGATTACTTTGAAGAAGATCCTAACAGTGCAATGGATAAAGAGAGACGA
AAGTCTTTACTAAAACCAAAATTATTGAGACTGCAGAGAGACATTGAAAAAGCCTCAAAA
GACAAGGAAGGCCTGGAACGAATGCTTAAAACGTACTCCAGCACCTCCTCCTTCTCTGAT
GCAAAGAGCCAGAAAGACACAGCAGCGTTAATGGATGAGAACAATTTGAAACTAGACCTT
TTGGAAGCGAACTCCTACAAACTGTCATCAATGTTAGCAGAACTTGAGCAAAGACCTCAA
CCCAGCCATCCTTGTAGTAATTCCATCTTCAGGTGGAGGGAAAAGGAGCATACTCATAGC
TATGTGAAAATATCTCGGCCTTTTTTAATGAAGAGATTAGAGAATATTGTGAGCAAGGCA
TCTTCTGGTGGGCAGAGCAATCCAGGTTCTTCAACTCCAGCCCCTGGTGCAGCCCAGCTC
AGCAGCAGACTTTGCAAGGCCTTGTATTCTTTTCAAGCCAGGCAAGATGATGAGTTGAAT
TTGGAAAAGGGTGACATTGTGATTATACACGAGAAAAAAGAAGAAGGATGGTGGTTTGGA
TCTTTGAATGGGAAAAAAGGCCATTTTCCTGCCGCTTATGTGGAGGAGTTACCTTCAAAT
GCTGGCAACACAGCTACAAAGGCA

ORF Protein

Cloned ORF protein sequence for pFN21AE1450 Download

>KIEE1450  428 aa
MKSTADLHQKLGKAIELEAIKPTYQVLNVQEKKRKSLDNEVEKTANLVISNWNQQIKAKK
KLMVSTKKHEALFQLVESSKQSMTEKEKRKLLNKLTKSTEKLEKEDENYYQKNMAGYSTR
LKWENTLENCYQSILELEKERIQLLCNNLNQYSQHISLFGQTLTTCHTQIHCAISKIDIE
KDIQAVMEETAILSTENKSEFLLTDYFEEDPNSAMDKERRKSLLKPKLLRLQRDIEKASK
DKEGLERMLKTYSSTSSFSDAKSQKDTAALMDENNLKLDLLEANSYKLSSMLAELEQRPQ
PSHPCSNSIFRWREKEHTHSYVKISRPFLMKRLENIVSKASSGGQSNPGSSTPAPGAAQL
SSRLCKALYSFQARQDDELNLEKGDIVIIHEKKEEGWWFGSLNGKKGHFPAAYVEELPSN
AGNTATKA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1450 Download

>pFN21AE1450  5989 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAATCCACAGCGGACCTGCATCAAAAACTTGG
CAAAGCAATTGAATTGGAAGCAATAAAACCGACTTATCAAGTCCTAAATGTACAAGAGAA
GAAGAGAAAATCACTTGACAATGAAGTTGAAAAGACAGCAAATCTTGTCATTAGCAACTG
GAATCAGCAAATTAAGGCCAAGAAGAAATTAATGGTTAGTACCAAGAAACATGAAGCACT
TTTCCAGCTTGTAGAAAGCTCCAAGCAATCTATGACTGAGAAGGAGAAGCGGAAGCTCCT
CAATAAACTGACAAAATCAACTGAAAAGTTGGAAAAGGAAGATGAAAATTACTACCAAAA
AAACATGGCGGGTTATTCTACCAGACTGAAATGGGAAAACACACTAGAGAACTGCTACCA
GAGCATTCTGGAGCTGGAGAAGGAAAGAATTCAACTTTTATGCAATAACTTAAACCAGTA
CAGCCAACATATTTCTCTTTTTGGCCAAACCCTGACCACATGCCACACGCAGATTCACTG
TGCCATCAGCAAGATTGACATTGAAAAAGATATCCAGGCTGTAATGGAAGAAACTGCAAT
TTTATCTACAGAAAACAAATCTGAGTTCCTGTTAACGGATTACTTTGAAGAAGATCCTAA
CAGTGCAATGGATAAAGAGAGACGAAAGTCTTTACTAAAACCAAAATTATTGAGACTGCA
GAGAGACATTGAAAAAGCCTCAAAAGACAAGGAAGGCCTGGAACGAATGCTTAAAACGTA
CTCCAGCACCTCCTCCTTCTCTGATGCAAAGAGCCAGAAAGACACAGCAGCGTTAATGGA
TGAGAACAATTTGAAACTAGACCTTTTGGAAGCGAACTCCTACAAACTGTCATCAATGTT
AGCAGAACTTGAGCAAAGACCTCAACCCAGCCATCCTTGTAGTAATTCCATCTTCAGGTG
GAGGGAAAAGGAGCATACTCATAGCTATGTGAAAATATCTCGGCCTTTTTTAATGAAGAG
ATTAGAGAATATTGTGAGCAAGGCATCTTCTGGTGGGCAGAGCAATCCAGGTTCTTCAAC
TCCAGCCCCTGGTGCAGCCCAGCTCAGCAGCAGACTTTGCAAGGCCTTGTATTCTTTTCA
AGCCAGGCAAGATGATGAGTTGAATTTGGAAAAGGGTGACATTGTGATTATACACGAGAA
AAAAGAAGAAGGATGGTGGTTTGGATCTTTGAATGGGAAAAAAGGCCATTTTCCTGCCGC
TTATGTGGAGGAGTTACCTTCAAATGCTGGCAACACAGCTACAAAGGCAGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT