MMP10 ORF clone FHC08085

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC08085

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Clone info.

Species

human

Clone Name

pFN21AE2236 Sequence (with vector) Restriction Map

Product ID

FHC08085

ORF Sequence

DNA sequence (1428 bp)
Protein sequence (476 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2236

Symbol

MMP10

Alias : SL-2, STMY2

Description

matrix metallopeptidase 10

Original Clone ID

cs03336

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1428 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 476 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8321.1 1.9e-206 100.0 MMP10 CCDS8323.1 2.6e-165 78.2 MMP3 CCDS73375.1 2.3e-99 49.6 MMP12 CCDS8318.1 5.6e-87 45.6 MMP20 CCDS8324.1 6.1e-59 49.7 MMP13 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR021190 86 99 PR00138 Peptidase M10A IPR021190 133 148 PR00138 Peptidase M10A IPR021190 156 184 PR00138 Peptidase M10A IPR021190 214 239 PR00138 Peptidase M10A IPR021190 250 263 PR00138 Peptidase M10A HMMPfam IPR002477 33 86 PF01471 Peptidoglycan binding-like IPR001818 107 263 PF00413 Peptidase M10 IPR018487 295 337 PF00045 Hemopexin/matrixin IPR018487 339 382 PF00045 Hemopexin/matrixin IPR018487 387 433 PF00045 Hemopexin/matrixin IPR018487 436 476 PF00045 Hemopexin/matrixin HMMSmart IPR006026 104 264 SM00235 Peptidase IPR018487 295 337 SM00120 Hemopexin/matrixin IPR018487 339 382 SM00120 Hemopexin/matrixin IPR018487 387 434 SM00120 Hemopexin/matrixin IPR018487 436 476 SM00120 Hemopexin/matrixin
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 2 MHLAFLVLLCLPVCSAYPLSGAA 24 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002416 476 185260 100.0 100.0 NP_002413 477 185250,614466 78.2 100.0 NP_002417 470 601046 49.6 99.8 NP_004762 483 604629,612529 45.6 99.4 NP_002418 471 250400,600108,602111 49.7 99.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2236 Download

>KIEE2236  1428 bp
ATGATGCATCTTGCATTCCTTGTGCTGTTGTGTCTGCCAGTCTGCTCTGCCTATCCTCTG
AGTGGGGCAGCAAAAGAGGAGGACTCCAACAAGGATCTTGCCCAGCAATACCTAGAAAAG
TACTACAACCTCGAAAAGGATGTGAAACAGTTTAGAAGAAAGGACAGTAATCTCATTGTT
AAAAAAATCCAAGGAATGCAGAAGTTCCTTGGGTTGGAGGTGACAGGGAAGCTAGACACT
GACACTCTGGAGGTGATGCGCAAGCCCAGGTGTGGAGTTCCTGACGTTGGTCACTTCAGC
TCCTTTCCTGGCATGCCGAAGTGGAGGAAAACCCACCTTACATACAGGATTGTGAATTAT
ACACCAGATTTGCCAAGAGATGCTGTTGATTCTGCCATTGAGAAAGCTCTGAAAGTCTGG
GAAGAGGTGACTCCACTCACATTCTCCAGGCTGTATGAAGGAGAGGCTGATATAATGATC
TCTTTTGCAGTTAAAGAACATGGAGACTTTTACTCTTTTGATGGCCCAGGACACAGTTTG
GCTCATGCCTACCCACCTGGACCTGGGCTTTATGGAGATATTCACTTTGATGATGATGAA
AAATGGACAGAAGATGCATCAGGCACCAATTTATTCCTCGTTGCTGCTCATGAACTTGGC
CACTCCCTGGGGCTCTTTCACTCAGCCAACACTGAAGCTTTGATGTACCCACTCTACAAC
TCATTCACAGAGCTCGCCCAGTTCCGCCTTTCGCAAGATGATGTGAATGGCATTCAGTCT
CTCTACGGACCTCCCCCTGCCTCTACTGAGGAACCCCTGGTGCCCACAAAATCTGTTCCT
TCGGGATCTGAGATGCCAGCCAAGTGTGATCCTGCTTTGTCCTTCGATGCCATCAGCACT
CTGAGGGGAGAATATCTGTTCTTTAAAGACAGATATTTTTGGCGAAGATCCCACTGGAAC
CCTGAACCTGAATTTCATTTGATTTCTGCATTTTGGCCCTCTCTTCCATCATATTTGGAT
GCTGCATATGAAGTTAACAGCAGGGACACCGTTTTTATTTTTAAAGGAAATGAGTTCTGG
GCCATCAGAGGAAATGAGGTACAAGCAGGTTATCCAAGAGGCATCCATACCCTGGGTTTT
CCTCCAACCATAAGGAAAATTGATGCAGCTGTTTCTGACAAGGAAAAGAAGAAAACATAC
TTCTTTGCAGCGGACAAATACTGGAGATTTGATGAAAATAGCCAGTCCATGGAGCAAGGC
TTCCCTAGACTAATAGCTGATGACTTTCCAGGAGTTGAGCCTAAGGTTGATGCTGTATTA
CAGGCATTTGGATTTTTCTACTTCTTCAGTGGATCATCACAGTTTGAGTTTGACCCCAAT
GCCAGGATGGTGACACACATATTAAAGAGTAACAGCTGGTTACATTGC

ORF Protein

Cloned ORF protein sequence for pFN21AE2236 Download

>KIEE2236  476 aa
MMHLAFLVLLCLPVCSAYPLSGAAKEEDSNKDLAQQYLEKYYNLEKDVKQFRRKDSNLIV
KKIQGMQKFLGLEVTGKLDTDTLEVMRKPRCGVPDVGHFSSFPGMPKWRKTHLTYRIVNY
TPDLPRDAVDSAIEKALKVWEEVTPLTFSRLYEGEADIMISFAVKEHGDFYSFDGPGHSL
AHAYPPGPGLYGDIHFDDDEKWTEDASGTNLFLVAAHELGHSLGLFHSANTEALMYPLYN
SFTELAQFRLSQDDVNGIQSLYGPPPASTEEPLVPTKSVPSGSEMPAKCDPALSFDAIST
LRGEYLFFKDRYFWRRSHWNPEPEFHLISAFWPSLPSYLDAAYEVNSRDTVFIFKGNEFW
AIRGNEVQAGYPRGIHTLGFPPTIRKIDAAVSDKEKKKTYFFAADKYWRFDENSQSMEQG
FPRLIADDFPGVEPKVDAVLQAFGFFYFFSGSSQFEFDPNARMVTHILKSNSWLHC

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2236 Download

>pFN21AE2236  6133 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATGCATCTTGCATTCCTTGTGCTGTTGTGTCT
GCCAGTCTGCTCTGCCTATCCTCTGAGTGGGGCAGCAAAAGAGGAGGACTCCAACAAGGA
TCTTGCCCAGCAATACCTAGAAAAGTACTACAACCTCGAAAAGGATGTGAAACAGTTTAG
AAGAAAGGACAGTAATCTCATTGTTAAAAAAATCCAAGGAATGCAGAAGTTCCTTGGGTT
GGAGGTGACAGGGAAGCTAGACACTGACACTCTGGAGGTGATGCGCAAGCCCAGGTGTGG
AGTTCCTGACGTTGGTCACTTCAGCTCCTTTCCTGGCATGCCGAAGTGGAGGAAAACCCA
CCTTACATACAGGATTGTGAATTATACACCAGATTTGCCAAGAGATGCTGTTGATTCTGC
CATTGAGAAAGCTCTGAAAGTCTGGGAAGAGGTGACTCCACTCACATTCTCCAGGCTGTA
TGAAGGAGAGGCTGATATAATGATCTCTTTTGCAGTTAAAGAACATGGAGACTTTTACTC
TTTTGATGGCCCAGGACACAGTTTGGCTCATGCCTACCCACCTGGACCTGGGCTTTATGG
AGATATTCACTTTGATGATGATGAAAAATGGACAGAAGATGCATCAGGCACCAATTTATT
CCTCGTTGCTGCTCATGAACTTGGCCACTCCCTGGGGCTCTTTCACTCAGCCAACACTGA
AGCTTTGATGTACCCACTCTACAACTCATTCACAGAGCTCGCCCAGTTCCGCCTTTCGCA
AGATGATGTGAATGGCATTCAGTCTCTCTACGGACCTCCCCCTGCCTCTACTGAGGAACC
CCTGGTGCCCACAAAATCTGTTCCTTCGGGATCTGAGATGCCAGCCAAGTGTGATCCTGC
TTTGTCCTTCGATGCCATCAGCACTCTGAGGGGAGAATATCTGTTCTTTAAAGACAGATA
TTTTTGGCGAAGATCCCACTGGAACCCTGAACCTGAATTTCATTTGATTTCTGCATTTTG
GCCCTCTCTTCCATCATATTTGGATGCTGCATATGAAGTTAACAGCAGGGACACCGTTTT
TATTTTTAAAGGAAATGAGTTCTGGGCCATCAGAGGAAATGAGGTACAAGCAGGTTATCC
AAGAGGCATCCATACCCTGGGTTTTCCTCCAACCATAAGGAAAATTGATGCAGCTGTTTC
TGACAAGGAAAAGAAGAAAACATACTTCTTTGCAGCGGACAAATACTGGAGATTTGATGA
AAATAGCCAGTCCATGGAGCAAGGCTTCCCTAGACTAATAGCTGATGACTTTCCAGGAGT
TGAGCCTAAGGTTGATGCTGTATTACAGGCATTTGGATTTTTCTACTTCTTCAGTGGATC
ATCACAGTTTGAGTTTGACCCCAATGCCAGGATGGTGACACACATATTAAAGAGTAACAG
CTGGTTACATTGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT