FHC07848

Gene/Protein Characteristic Table for FHC07848

Species

human

Clone Name

pFN21AB7967 Sequence (with vector) Restriction Map

Product ID

FHC07848

Corresponding
Flexi Clone PID

FXC07848

ORF Sequence

DNA sequence (849 bp)
Protein sequence (283 aa)

Linker Type

Flexi type

The extra Val codon is attached to the 3'-end of the ORF, because a Pme I site ...

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528304]

Gene

KIBB7967

Symbol

KLF6

Alias : BCD1, CBA1, COPEB, CPBP, GBF, PAC1, ST12, ZF9

Description

Krueppel-like factor 6 (Core promoter element-binding protein)(B-cell-derived protein 1)(Proto-oncogene BCD1)(Transcription factor Zf9)(GC-rich sites-binding factor GBF)

Original Clone ID

cs00467

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 849 bp
Restriction map A Restriction map B

Features of the protein sequence

Description

Length: 283 aa
Result of homology search against nr database ( FASTA output, Multiple alignment) Entry Exp ID% Protein Source Q99612 2.2e-107 100.0 Krueppel-like f... Homo sapiens AAP36589 2.2e-107 100.0 core promoter e... synthetic construct AAC23699 2.2e-107 100.0 DNA-binding pro... Homo sapiens XP_001141364 2.5e-107 99.6 similar to DNA-... Pan troglodytes AAC39929 4.3e-107 99.6 Kruppel-like zi... Homo sapiens The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition BlastProDom IPR007087 230 255 PD000003 Zinc finger IPR007087 260 282 PD000003 Zinc finger HMMPfam IPR007087 200 224 PF00096 Zinc finger IPR007087 230 254 PF00096 Zinc finger IPR007087 260 282 PF00096 Zinc finger HMMSmart IPR015880 200 224 SM00355 Zinc finger IPR015880 230 254 SM00355 Zinc finger IPR015880 260 282 SM00355 Zinc finger ProfileScan IPR007087 200 229 PS50157 Zinc finger IPR007087 230 259 PS50157 Zinc finger IPR007087 260 283 PS50157 Zinc finger ScanRegExp IPR007087 202 224 PS00028 Zinc finger IPR007087 232 254 PS00028 Zinc finger IPR007087 262 282 PS00028 Zinc finger
Result of homology search against disease genes in OMIM database

(FASTA output, Multiple alignment)

H_id	H_len (aa)	OMIM_No.	ID (%)	Q_ali/Q_len (%)
NP_001291	283	137215,176807,602053	100.0	100.0
NP_001153597	237	137215,176807,602053	97.8	82.7
NP_001153596	241	137215,176807,602053	85.1	100.0
NP_003700	302	604865	66.7	100.0