PLEKHF2 ORF clone FHC07733

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC07733

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Clone info.

Species

human

Clone Name

pFN21AB7109 Sequence (with vector) Restriction Map

Product ID

FHC07733

Flexi Clone PID

FXC07733

ORF Sequence

DNA sequence (744 bp)
Protein sequence (248 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464029]

Gene

KIBB7109

Symbol

PLEKHF2

Alias : EAPF, PHAFIN2, ZFYVE18

Description

pleckstrin homology domain containing, family F (with FYVE domain) member 2

Original Clone ID

cp01114

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 744 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 248 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6267.1 1.1e-91 99.6 PLEKHF2 CCDS12417.1 5.4e-52 56.2 PLEKHF1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 38 130 PF00169 Pleckstrin homology domain IPR000306 148 211 PF01363 Zinc finger HMMSmart IPR001849 36 133 SM00233 Pleckstrin homology domain IPR000306 144 213 SM00064 Zinc finger ProfileScan IPR001849 35 131 PS50003 Pleckstrin homology domain IPR017455 152 212 PS50178 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_078889 249 615208 99.6 100.0 XP_011525611 279 615200 56.2 100.0 NP_077286 279 615200 56.2 100.0 XP_005259313 364 615200 56.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7109 Download

>KIBB7109  744 bp
ATGGTGGATCGCTTGGCAAACAGTGAAGCAAATACTAGACGTATAAGTATAGTGGAAAAC
TGTTTTGGAGCAGCTGGTCAACCTTTAACTATACCTGGACGAGTTCTTATTGGAGAAGGA
GTATTGACTAAGTTGTGCAGGAAAAAGCCCAAAGCAAGGCAGTTTTTCTTGTTTAATGAT
ATTCTTGTATATGGCAATATTGTCATCCAGAAGAAAAAATATAACAAACAACATATTATT
CCCCTGGAAAATGTCACTATTGATTCCATCAAAGATGAGGGAGACTTAAGGAATGGATGG
CTAATCAAGACACCAACTAAATCTTTTGCAGTTTATGCTGCCACTGCTACGGAGAAATCA
GAATGGATGAATCATATAAATAAATGTGTTACTGATTTACTCTCCAAAAGTGGGAAGACA
CCCAGTAATGAACATGCTGCTGTCTGGGTTCCTGACTCTGAGGCAACTGTATGTATGCGT
TGTCAGAAAGCAAAATTCACACCTGTTAATCGTCGCCACCATTGCCGCAAATGTGGTTTT
GTTGTCTGTGGGCCCTGCTCTGAAAAGAGATTTCTTCTTCCCAGCCAGTCCTCTAAGCCT
GTGCGGATTTGTGACTTCTGCTATGACCTGCTTTCTGCTGGGGACATGGCCACATGCCAG
CCTGCTAGATCAGACTCTTACAGCCAGTCATTGAAGTCTCCTTTAAATGATATGTCTGAT
GATGACGATGATGATAGCAGTGAC

ORF Protein

Cloned ORF protein sequence for pFN21AB7109 Download

>KIBB7109  248 aa
MVDRLANSEANTRRISIVENCFGAAGQPLTIPGRVLIGEGVLTKLCRKKPKARQFFLFND
ILVYGNIVIQKKKYNKQHIIPLENVTIDSIKDEGDLRNGWLIKTPTKSFAVYAATATEKS
EWMNHINKCVTDLLSKSGKTPSNEHAAVWVPDSEATVCMRCQKAKFTPVNRRHHCRKCGF
VVCGPCSEKRFLLPSQSSKPVRICDFCYDLLSAGDMATCQPARSDSYSQSLKSPLNDMSD
DDDDDSSD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7109 Download

>pFN21AB7109  5449 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTGGATCGCTTGGCAAACAGTGAAGCAAATAC
TAGACGTATAAGTATAGTGGAAAACTGTTTTGGAGCAGCTGGTCAACCTTTAACTATACC
TGGACGAGTTCTTATTGGAGAAGGAGTATTGACTAAGTTGTGCAGGAAAAAGCCCAAAGC
AAGGCAGTTTTTCTTGTTTAATGATATTCTTGTATATGGCAATATTGTCATCCAGAAGAA
AAAATATAACAAACAACATATTATTCCCCTGGAAAATGTCACTATTGATTCCATCAAAGA
TGAGGGAGACTTAAGGAATGGATGGCTAATCAAGACACCAACTAAATCTTTTGCAGTTTA
TGCTGCCACTGCTACGGAGAAATCAGAATGGATGAATCATATAAATAAATGTGTTACTGA
TTTACTCTCCAAAAGTGGGAAGACACCCAGTAATGAACATGCTGCTGTCTGGGTTCCTGA
CTCTGAGGCAACTGTATGTATGCGTTGTCAGAAAGCAAAATTCACACCTGTTAATCGTCG
CCACCATTGCCGCAAATGTGGTTTTGTTGTCTGTGGGCCCTGCTCTGAAAAGAGATTTCT
TCTTCCCAGCCAGTCCTCTAAGCCTGTGCGGATTTGTGACTTCTGCTATGACCTGCTTTC
TGCTGGGGACATGGCCACATGCCAGCCTGCTAGATCAGACTCTTACAGCCAGTCATTGAA
GTCTCCTTTAAATGATATGTCTGATGATGACGATGATGATAGCAGTGACGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT