TMEM104 ORF clone FHC07132

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Halotag ORF Clone FHC07132

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Clone info.

Species

human

Clone Name

pFN21AF0021 Sequence (with vector) Restriction Map

Product ID

FHC07132

Flexi Clone PID

FXC07132

ORF Sequence

DNA sequence (1488 bp)
Protein sequence (496 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527228]

Gene

FLJ00021

Symbol

TMEM104

Description

transmembrane protein 104

Original Clone ID

as00021

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1488 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 496 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32723.1 0 100.0 TMEM104 CCDS82201.1 3.8e-110 100.0 TMEM104 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013057 18 66 PF01490 Amino acid transporter IPR013057 134 454 PF01490 Amino acid transporter
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 11 LYSSYVGLVYMFNLIVGTGALTM 33 PRIMARY 23 2 45 SLVLLVFLGFMSFVTTTFVIEAM 67 PRIMARY 23 3 150 FCIIVYLYGDLAIYAAAVPFSLM 172 PRIMARY 23 4 207 AYRIYLAIFTLLLGPFTFFDVQK 229 SECONDARY 23 5 235 ILTSLMRWIAFAVMIVLALIRIG 257 PRIMARY 23 6 309 LDYVLILAFYGLLSFTAIFCFRG 331 PRIMARY 23 7 349 GLAAVRFFLGLFPVFTISTNFPI 371 SECONDARY 23 8 393 WVVDRVVFPTITLVPPVLVAFCT 415 SECONDARY 23 9 422 VGITGAYAGTGIQYVIPAFLVYH 444 SECONDARY 23 10 470 WVGFVLLWAFSCFIFVTANIILS 492 PRIMARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AF0021 Download

>FLJ00021  1488 bp
ATGGCGGGTGAAATTACAGAGACCGGGGAACTCTACTCTTCCTACGTTGGGCTGGTGTAC
ATGTTTAACCTCATCGTGGGCACGGGCGCACTCACCATGCCCAAGGCATTCGCCACTGCC
GGGTGGCTTGTCAGCCTCGTCCTGCTGGTGTTCCTGGGCTTCATGAGCTTCGTGACCACC
ACCTTTGTGATAGAGGCCATGGCTGCAGCCAACGCGCAGCTCCACTGGAAGAGGATGGAG
AACCTCAAGGAGGAGGAAGATGACGACTCCTCCACAGCCTCAGACAGCGATGTTCTCATC
CGGGACAACTACGAGCGGGCAGAGAAGCGGCCCATCCTGTCTGTGCAGAGACGTGGATCT
CCCAACCCGTTTGAAATCACAGACCGGGTGGAAATGGGACAAATGGCCTCCATGTTCTTC
AATAAAGTGGGGGTCAACTTGTTCTATTTCTGCATCATCGTTTACCTGTATGGAGACCTC
GCCATCTATGCTGCTGCCGTGCCCTTCTCCCTCATGCAGGTGACCTGCAGCGCCACTGGC
AATGACTCCTGCGGTGTGGAAGCAGACACCAAATACAATGACACTGACCGGTGCTGGGGG
CCCCTGCGCCGAGTGGACGCCTACCGCATCTACTTGGCGATCTTCACTCTCCTCCTCGGA
CCGTTCACCTTCTTTGACGTCCAGAAGACCAAGTACCTGCAGATCCTGACCTCTCTGATG
AGATGGATCGCTTTCGCCGTCATGATTGTGCTGGCCCTGATCCGCATCGGGCACGGACAA
GGGGAGGGGCACCCGCCCCTGGCTGACTTCTCGGGGGTCCGGAACCTGTTTGGGGTGTGC
GTCTACTCCTTCATGTGCCAGCACTCTCTGCCATCCCTCATTACCCCCGTCTCCTCCAAG
CGCCACCTCACAAGGCTGGTGTTCCTGGACTACGTGCTGATCCTGGCCTTCTACGGCCTC
CTCTCCTTCACCGCCATCTTCTGCTTCCGCGGCGACAGCCTCATGGACATGTACACCCTC
AACTTCGCGCGCTGTGACGTCGTGGGCCTGGCCGCTGTGCGCTTTTTCCTGGGCCTCTTC
CCCGTCTTCACCATCAGCACCAACTTCCCCATCATTGCCGTGACCCTGCGCAACAACTGG
AAGACACTCTTCCACCGCGAGGGCGGCACGTACCCGTGGGTGGTGGACCGCGTCGTGTTT
CCCACCATCACCCTGGTGCCGCCTGTGCTGGTGGCCTTCTGCACCCACGACCTGGAGTCC
CTGGTGGGCATCACAGGGGCCTACGCGGGCACCGGCATCCAGTACGTCATCCCCGCCTTC
CTGGTGTACCACTGCCGCAGGGACACCCAGCTGGCCTTTGGCTGTGGGGTCAGCAACAAG
CACAGGTCCCCTTTCCGCCACACCTTCTGGGTGGGCTTCGTGCTGCTCTGGGCTTTCTCC
TGCTTCATTTTCGTCACGGCCAATATCATCCTCAGCGAGACCAAGCTC

ORF Protein

Cloned ORF protein sequence for pFN21AF0021 Download

>FLJ00021  496 aa
MAGEITETGELYSSYVGLVYMFNLIVGTGALTMPKAFATAGWLVSLVLLVFLGFMSFVTT
TFVIEAMAAANAQLHWKRMENLKEEEDDDSSTASDSDVLIRDNYERAEKRPILSVQRRGS
PNPFEITDRVEMGQMASMFFNKVGVNLFYFCIIVYLYGDLAIYAAAVPFSLMQVTCSATG
NDSCGVEADTKYNDTDRCWGPLRRVDAYRIYLAIFTLLLGPFTFFDVQKTKYLQILTSLM
RWIAFAVMIVLALIRIGHGQGEGHPPLADFSGVRNLFGVCVYSFMCQHSLPSLITPVSSK
RHLTRLVFLDYVLILAFYGLLSFTAIFCFRGDSLMDMYTLNFARCDVVGLAAVRFFLGLF
PVFTISTNFPIIAVTLRNNWKTLFHREGGTYPWVVDRVVFPTITLVPPVLVAFCTHDLES
LVGITGAYAGTGIQYVIPAFLVYHCRRDTQLAFGCGVSNKHRSPFRHTFWVGFVLLWAFS
CFIFVTANIILSETKL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AF0021 Download

>pFN21AF0021  6193 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGGTGAAATTACAGAGACCGGGGAACTCTA
CTCTTCCTACGTTGGGCTGGTGTACATGTTTAACCTCATCGTGGGCACGGGCGCACTCAC
CATGCCCAAGGCATTCGCCACTGCCGGGTGGCTTGTCAGCCTCGTCCTGCTGGTGTTCCT
GGGCTTCATGAGCTTCGTGACCACCACCTTTGTGATAGAGGCCATGGCTGCAGCCAACGC
GCAGCTCCACTGGAAGAGGATGGAGAACCTCAAGGAGGAGGAAGATGACGACTCCTCCAC
AGCCTCAGACAGCGATGTTCTCATCCGGGACAACTACGAGCGGGCAGAGAAGCGGCCCAT
CCTGTCTGTGCAGAGACGTGGATCTCCCAACCCGTTTGAAATCACAGACCGGGTGGAAAT
GGGACAAATGGCCTCCATGTTCTTCAATAAAGTGGGGGTCAACTTGTTCTATTTCTGCAT
CATCGTTTACCTGTATGGAGACCTCGCCATCTATGCTGCTGCCGTGCCCTTCTCCCTCAT
GCAGGTGACCTGCAGCGCCACTGGCAATGACTCCTGCGGTGTGGAAGCAGACACCAAATA
CAATGACACTGACCGGTGCTGGGGGCCCCTGCGCCGAGTGGACGCCTACCGCATCTACTT
GGCGATCTTCACTCTCCTCCTCGGACCGTTCACCTTCTTTGACGTCCAGAAGACCAAGTA
CCTGCAGATCCTGACCTCTCTGATGAGATGGATCGCTTTCGCCGTCATGATTGTGCTGGC
CCTGATCCGCATCGGGCACGGACAAGGGGAGGGGCACCCGCCCCTGGCTGACTTCTCGGG
GGTCCGGAACCTGTTTGGGGTGTGCGTCTACTCCTTCATGTGCCAGCACTCTCTGCCATC
CCTCATTACCCCCGTCTCCTCCAAGCGCCACCTCACAAGGCTGGTGTTCCTGGACTACGT
GCTGATCCTGGCCTTCTACGGCCTCCTCTCCTTCACCGCCATCTTCTGCTTCCGCGGCGA
CAGCCTCATGGACATGTACACCCTCAACTTCGCGCGCTGTGACGTCGTGGGCCTGGCCGC
TGTGCGCTTTTTCCTGGGCCTCTTCCCCGTCTTCACCATCAGCACCAACTTCCCCATCAT
TGCCGTGACCCTGCGCAACAACTGGAAGACACTCTTCCACCGCGAGGGCGGCACGTACCC
GTGGGTGGTGGACCGCGTCGTGTTTCCCACCATCACCCTGGTGCCGCCTGTGCTGGTGGC
CTTCTGCACCCACGACCTGGAGTCCCTGGTGGGCATCACAGGGGCCTACGCGGGCACCGG
CATCCAGTACGTCATCCCCGCCTTCCTGGTGTACCACTGCCGCAGGGACACCCAGCTGGC
CTTTGGCTGTGGGGTCAGCAACAAGCACAGGTCCCCTTTCCGCCACACCTTCTGGGTGGG
CTTCGTGCTGCTCTGGGCTTTCTCCTGCTTCATTTTCGTCACGGCCAATATCATCCTCAG
CGAGACCAAGCTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT