SLC5A4 ORF clone FHC06885

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC06885

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Clone info.

Species

human

Clone Name

pFN21AE0360 Sequence (with vector) Restriction Map

Product ID

FHC06885

Flexi Clone PID

FXC06885

ORF Sequence

DNA sequence (1977 bp)
Protein sequence (659 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527216]

Gene

KIEE0360

Symbol

SLC5A4

Alias : DJ90G24.4, SAAT1, SGLT3

Description

solute carrier family 5 (glucose activated ion channel), member 4

Original Clone ID

oc01060

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1977 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 659 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13903.1 0 100.0 SLC5A4 CCDS13902.1 5.6e-215 70.4 SLC5A1 CCDS58805.1 1.9e-173 70.4 SLC5A1 CCDS10714.1 3.8e-164 56.5 SLC5A2 CCDS30709.2 1.6e-143 51.8 SLC5A9 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001734 58 492 PF00474 Sodium/solute symporter HMMTigr IPR019900 58 492 TIGR00813 Sodium/solute symporter ProfileScan IPR001734 25 506 PS50283 Sodium/solute symporter
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 26 AADISVIVIYFLVVMAVGLWAM 47 PRIMARY 22 2 107 VMLLILGWIFVPIYIKSGVMTMP 129 PRIMARY 23 3 142 QVYLSILSLFICVVLLISADIFA 164 PRIMARY 23 4 170 KLALGLDLYLAIFILLAMTAVYT 192 PRIMARY 23 5 205 TLQTIIMLIGSFILMGFAFNEVG 227 PRIMARY 23 6 272 GDIPWPGIIFGMPITALWYWCTN 294 SECONDARY 23 7 308 SHVKAACIMCAYLKLLPMFLMVM 330 PRIMARY 23 8 334 ISRILYTDMVACVVPSECVKHCG 356 SECONDARY 23 9 383 LSVMLASLMSSLTSIFNSASTLF 405 SECONDARY 23 10 421 ELLIAGRIFVLLLTVVSIVWVPL 443 PRIMARY 23 11 457 ESISSYLGPPIAAVFVLAIFCK 478 PRIMARY 22 12 485 AFWGLMVGLAMGLIRMITEFAYG 507 SECONDARY 23 13 525 HYLYFSIVLFFGSMLVTLGISLL 547 PRIMARY 23 14 635 SWRTIVNINAILLLAVVVFIHG 656 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000334 664 182380,606824 70.4 99.8 NP_001243243 537 182380,606824 70.4 80.7 NP_003032 672 182381,233100 56.5 98.0 XP_006721135 705 182381,233100 54.5 97.0 XP_016878389 675 610238 49.2 98.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0360 Download

>KIEE0360  1977 bp
ATGGCCAGTACGGTTAGCCCCAGCACCATAGCTGAGACCCCAGAGCCACCTCCATTGTCT
GACCACATCCGAAATGCTGCTGACATCTCAGTCATTGTCATCTATTTTCTGGTGGTGATG
GCTGTTGGGCTGTGGGCGATGCTGAAGACCAACCGAGGTACTATAGGAGGCTTCTTCCTC
GCTGGTCGTGATATGGCCTGGTGGCCGATGGGCGCCTCTCTCTTTGCCAGTAACATCGGC
AGCAACCACTATGTGGGGCTGGCTGGGACAGGAGCAGCTTCAGGAGTCGCCACCGTAACA
TTTGAATGGACTTCCTCAGTAATGTTGCTGATTCTTGGGTGGATCTTTGTCCCTATCTAC
ATCAAGTCGGGGGTGATGACCATGCCGGAATATCTCAAGAAGCGGTTTGGTGGGGAGCGA
CTCCAGGTCTACCTCTCCATCCTCTCCCTCTTCATCTGTGTGGTTTTGTTAATTTCTGCA
GACATATTTGCTGGAGCCATATTCATCAAGCTGGCCTTGGGATTGGACCTTTACCTGGCA
ATCTTCATCCTCTTGGCTATGACTGCTGTTTACACCACCACTGGGGGCTTGGCCTCGGTG
ATTTACACAGACACCCTCCAGACCATCATCATGCTGATTGGCTCTTTTATTCTCATGGGG
TTTGCATTTAACGAAGTTGGAGGTTATGAGAGCTTTACCGAGAAGTACGTGAATGCCACC
CCATCCGTAGTCGAGGGGGACAACTTGACAATCAGTGCCAGTTGCTACACACCTCGGGCG
GACTCCTTCCATATCTTCCGAGATGCTGTGACTGGGGACATTCCATGGCCAGGAATTATA
TTTGGAATGCCCATTACAGCTTTGTGGTACTGGTGCACAAATCAGGTCATTGTGCAGCGC
TGCCTGTGTGGCAAGGACATGTCTCACGTGAAGGCCGCTTGCATTATGTGTGCTTACCTG
AAGCTGCTGCCCATGTTCCTCATGGTGATGCCGGGGATGATCAGCCGCATCCTGTACACA
GATATGGTAGCATGTGTGGTACCTTCTGAATGCGTGAAACACTGTGGCGTTGATGTTGGC
TGCACCAACTACGCATACCCCACGATGGTGCTGGAACTGATGCCCCAAGGACTGCGAGGC
CTGATGCTTTCGGTCATGCTGGCCTCTCTCATGAGCTCCCTGACCTCCATCTTCAACAGC
GCCAGCACCCTCTTCACCATTGACCTCTACACCAAGATGCGGAAGCAAGCGTCGGAGAAA
GAGCTCCTGATAGCTGGACGGATATTTGTTCTTCTATTAACTGTTGTGAGCATTGTGTGG
GTCCCACTGGTACAAGTTTCTCAAAATGGACAACTAATCCATTACACAGAATCAATTTCT
AGCTACCTTGGGCCTCCAATTGCAGCTGTCTTTGTGCTTGCCATCTTCTGTAAAAGAGTC
AATGAACAGGGAGCATTCTGGGGTCTAATGGTTGGACTTGCAATGGGCCTCATTCGTATG
ATAACAGAGTTTGCTTATGGAACAGGGAGTTGCTTGGCTCCCAGTAACTGTCCCAAGATT
ATCTGTGGAGTGCACTATCTGTACTTTTCCATCGTTCTCTTTTTTGGGTCCATGCTGGTC
ACCCTGGGAATTTCCCTCTTAACAAAACCCATTCCTGATGTACATCTGTACCGCCTGTGC
TGGGTTCTTCGGAACAGTACAGAGGAGCGAATCGATATAGATGCAGAAGAGAAAAGTCAG
GAAGAAACAGATGATGGTGTTGAAGAAGATTATCCTGAGAAATCACGTGGATGCCTCAAG
AAAGCTTATGACTTGTTCTGCGGTTTGCAGAAGGGACCCAAGCTAACCAAGGAGGAGGAG
GAAGCCTTGAGCAAGAAGCTCACAGACACGTCTGAGAGGCCCTCGTGGAGGACAATAGTG
AACATCAACGCCATCCTCCTCCTGGCTGTGGTGGTCTTTATTCACGGCTACTATGCC

ORF Protein

Cloned ORF protein sequence for pFN21AE0360 Download

>KIEE0360  659 aa
MASTVSPSTIAETPEPPPLSDHIRNAADISVIVIYFLVVMAVGLWAMLKTNRGTIGGFFL
AGRDMAWWPMGASLFASNIGSNHYVGLAGTGAASGVATVTFEWTSSVMLLILGWIFVPIY
IKSGVMTMPEYLKKRFGGERLQVYLSILSLFICVVLLISADIFAGAIFIKLALGLDLYLA
IFILLAMTAVYTTTGGLASVIYTDTLQTIIMLIGSFILMGFAFNEVGGYESFTEKYVNAT
PSVVEGDNLTISASCYTPRADSFHIFRDAVTGDIPWPGIIFGMPITALWYWCTNQVIVQR
CLCGKDMSHVKAACIMCAYLKLLPMFLMVMPGMISRILYTDMVACVVPSECVKHCGVDVG
CTNYAYPTMVLELMPQGLRGLMLSVMLASLMSSLTSIFNSASTLFTIDLYTKMRKQASEK
ELLIAGRIFVLLLTVVSIVWVPLVQVSQNGQLIHYTESISSYLGPPIAAVFVLAIFCKRV
NEQGAFWGLMVGLAMGLIRMITEFAYGTGSCLAPSNCPKIICGVHYLYFSIVLFFGSMLV
TLGISLLTKPIPDVHLYRLCWVLRNSTEERIDIDAEEKSQEETDDGVEEDYPEKSRGCLK
KAYDLFCGLQKGPKLTKEEEEALSKKLTDTSERPSWRTIVNINAILLLAVVVFIHGYYA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0360 Download

>pFN21AE0360  6682 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCAGTACGGTTAGCCCCAGCACCATAGCTGA
GACCCCAGAGCCACCTCCATTGTCTGACCACATCCGAAATGCTGCTGACATCTCAGTCAT
TGTCATCTATTTTCTGGTGGTGATGGCTGTTGGGCTGTGGGCGATGCTGAAGACCAACCG
AGGTACTATAGGAGGCTTCTTCCTCGCTGGTCGTGATATGGCCTGGTGGCCGATGGGCGC
CTCTCTCTTTGCCAGTAACATCGGCAGCAACCACTATGTGGGGCTGGCTGGGACAGGAGC
AGCTTCAGGAGTCGCCACCGTAACATTTGAATGGACTTCCTCAGTAATGTTGCTGATTCT
TGGGTGGATCTTTGTCCCTATCTACATCAAGTCGGGGGTGATGACCATGCCGGAATATCT
CAAGAAGCGGTTTGGTGGGGAGCGACTCCAGGTCTACCTCTCCATCCTCTCCCTCTTCAT
CTGTGTGGTTTTGTTAATTTCTGCAGACATATTTGCTGGAGCCATATTCATCAAGCTGGC
CTTGGGATTGGACCTTTACCTGGCAATCTTCATCCTCTTGGCTATGACTGCTGTTTACAC
CACCACTGGGGGCTTGGCCTCGGTGATTTACACAGACACCCTCCAGACCATCATCATGCT
GATTGGCTCTTTTATTCTCATGGGGTTTGCATTTAACGAAGTTGGAGGTTATGAGAGCTT
TACCGAGAAGTACGTGAATGCCACCCCATCCGTAGTCGAGGGGGACAACTTGACAATCAG
TGCCAGTTGCTACACACCTCGGGCGGACTCCTTCCATATCTTCCGAGATGCTGTGACTGG
GGACATTCCATGGCCAGGAATTATATTTGGAATGCCCATTACAGCTTTGTGGTACTGGTG
CACAAATCAGGTCATTGTGCAGCGCTGCCTGTGTGGCAAGGACATGTCTCACGTGAAGGC
CGCTTGCATTATGTGTGCTTACCTGAAGCTGCTGCCCATGTTCCTCATGGTGATGCCGGG
GATGATCAGCCGCATCCTGTACACAGATATGGTAGCATGTGTGGTACCTTCTGAATGCGT
GAAACACTGTGGCGTTGATGTTGGCTGCACCAACTACGCATACCCCACGATGGTGCTGGA
ACTGATGCCCCAAGGACTGCGAGGCCTGATGCTTTCGGTCATGCTGGCCTCTCTCATGAG
CTCCCTGACCTCCATCTTCAACAGCGCCAGCACCCTCTTCACCATTGACCTCTACACCAA
GATGCGGAAGCAAGCGTCGGAGAAAGAGCTCCTGATAGCTGGACGGATATTTGTTCTTCT
ATTAACTGTTGTGAGCATTGTGTGGGTCCCACTGGTACAAGTTTCTCAAAATGGACAACT
AATCCATTACACAGAATCAATTTCTAGCTACCTTGGGCCTCCAATTGCAGCTGTCTTTGT
GCTTGCCATCTTCTGTAAAAGAGTCAATGAACAGGGAGCATTCTGGGGTCTAATGGTTGG
ACTTGCAATGGGCCTCATTCGTATGATAACAGAGTTTGCTTATGGAACAGGGAGTTGCTT
GGCTCCCAGTAACTGTCCCAAGATTATCTGTGGAGTGCACTATCTGTACTTTTCCATCGT
TCTCTTTTTTGGGTCCATGCTGGTCACCCTGGGAATTTCCCTCTTAACAAAACCCATTCC
TGATGTACATCTGTACCGCCTGTGCTGGGTTCTTCGGAACAGTACAGAGGAGCGAATCGA
TATAGATGCAGAAGAGAAAAGTCAGGAAGAAACAGATGATGGTGTTGAAGAAGATTATCC
TGAGAAATCACGTGGATGCCTCAAGAAAGCTTATGACTTGTTCTGCGGTTTGCAGAAGGG
ACCCAAGCTAACCAAGGAGGAGGAGGAAGCCTTGAGCAAGAAGCTCACAGACACGTCTGA
GAGGCCCTCGTGGAGGACAATAGTGAACATCAACGCCATCCTCCTCCTGGCTGTGGTGGT
CTTTATTCACGGCTACTATGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT