SLC25A18 ORF clone FHC06855

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Halotag ORF Clone FHC06855

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Clone info.

Species

human

Clone Name

pFN21AE0543 Sequence (with vector) Restriction Map

Product ID

FHC06855

Flexi Clone PID

FXC06855

ORF Sequence

DNA sequence (945 bp)
Protein sequence (315 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528235]

Gene

KIEE0543

Symbol

SLC25A18

Alias : GC2

Description

solute carrier family 25 (glutamate carrier), member 18, transcript variant 1

Original Clone ID

oc01243

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 945 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 315 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13744.1 1.9e-143 100.0 SLC25A18 CCDS7715.1 8.8e-91 65.6 SLC25A22 CCDS5645.1 1.2e-21 43.4 SLC25A13 CCDS55130.1 1.2e-21 43.4 SLC25A13 CCDS33327.1 2.6e-21 44.1 SLC25A12 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002067 11 24 PR00926 Mitochondrial carrier protein IPR002067 24 38 PR00926 Mitochondrial carrier protein IPR002067 67 87 PR00926 Mitochondrial carrier protein IPR002067 183 201 PR00926 Mitochondrial carrier protein IPR002067 228 250 PR00926 Mitochondrial carrier protein HMMPfam IPR018108 5 95 PF00153 Mitochondrial substrate/solute carrier IPR018108 105 209 PF00153 Mitochondrial substrate/solute carrier IPR018108 218 302 PF00153 Mitochondrial substrate/solute carrier ProfileScan IPR018108 6 92 PS50920 Mitochondrial substrate/solute carrier IPR018108 100 210 PS50920 Mitochondrial substrate/solute carrier IPR018108 219 308 PS50920 Mitochondrial substrate/solute carrier
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 8 ITAKLINGGVAGLVGVTCVFPID 30 SECONDARY 23 2 225 FVSGCVAGSIAAVAVTPLDVLK 246 SECONDARY 22 3 288 ALVIAPLFGIAQGVYFIGIGERI 310 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016884457 315 609303 100.0 100.0 NP_001290413 315 609303 100.0 100.0 NP_113669 315 609303 100.0 100.0 XP_011544453 315 609303 100.0 100.0 XP_011544455 315 609303 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0543 Download

>KIEE0543  945 bp
ATGACCCACCAGGATCTGAGCATCACAGCCAAACTCATCAATGGAGGTGTAGCAGGGCTC
GTGGGGGTGACCTGCGTGTTCCCCATCGACTTGGCCAAGACTCGCCTGCAGAACCAGCAT
GGGAAAGCCATGTACAAAGGAATGATCGACTGCCTGATGAAGACGGCTCGGGCGGAGGGC
TTCTTCGGCATGTACCGAGGGGCTGCAGTGAACCTCACTCTGGTCACTCCAGAGAAGGCC
ATCAAGCTGGCGGCCAACGACTTTTTCCGGCGGCTGCTCATGGAAGATGGGATGCAGCGG
AACCTGAAGATGGAGATGCTTGCCGGGTGTGGGGCTGGGATGTGCCAGGTCGTGGTGACC
TGTCCCATGGAAATGCTCAAGATTCAGCTGCAGGATGCTGGACGCCTGGCCGTCCATCAT
CAGGGCTCGGCCTCAGCACCCTCCACCTCCAGGTCCTACACAACTGGTTCGGCTTCCACC
CACAGGCGCCCCTCTGCCACCCTCATTGCCTGGGAGCTGCTCCGCACTCAGGGCCTGGCT
GGGCTCTACAGGGGCCTGGGTGCCACTCTCCTCAGAGACATTCCTTTCTCCATCATCTAC
TTCCCACTGTTTGCCAACCTTAACAACCTGGGGTTCAACGAGCTCGCCGGTAAGGCGTCC
TTTGCACATTCCTTCGTGTCAGGCTGTGTGGCAGGTTCCATAGCTGCGGTCGCAGTGACG
CCTCTAGATGTTCTGAAAACTCGAATCCAAACCCTCAAGAAAGGCCTGGGCGAGGACATG
TACAGTGGGATCACCGACTGTGCCAGGAAACTCTGGATTCAGGAGGGACCATCTGCCTTC
ATGAAAGGCGCTGGCTGCCGGGCACTGGTCATAGCACCTCTCTTTGGGATTGCTCAAGGG
GTCTATTTTATTGGGATTGGAGAGCGCATCTTAAAGTGTTTTGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE0543 Download

>KIEE0543  315 aa
MTHQDLSITAKLINGGVAGLVGVTCVFPIDLAKTRLQNQHGKAMYKGMIDCLMKTARAEG
FFGMYRGAAVNLTLVTPEKAIKLAANDFFRRLLMEDGMQRNLKMEMLAGCGAGMCQVVVT
CPMEMLKIQLQDAGRLAVHHQGSASAPSTSRSYTTGSASTHRRPSATLIAWELLRTQGLA
GLYRGLGATLLRDIPFSIIYFPLFANLNNLGFNELAGKASFAHSFVSGCVAGSIAAVAVT
PLDVLKTRIQTLKKGLGEDMYSGITDCARKLWIQEGPSAFMKGAGCRALVIAPLFGIAQG
VYFIGIGERILKCFD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0543 Download

>pFN21AE0543  5650 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACCCACCAGGATCTGAGCATCACAGCCAAACT
CATCAATGGAGGTGTAGCAGGGCTCGTGGGGGTGACCTGCGTGTTCCCCATCGACTTGGC
CAAGACTCGCCTGCAGAACCAGCATGGGAAAGCCATGTACAAAGGAATGATCGACTGCCT
GATGAAGACGGCTCGGGCGGAGGGCTTCTTCGGCATGTACCGAGGGGCTGCAGTGAACCT
CACTCTGGTCACTCCAGAGAAGGCCATCAAGCTGGCGGCCAACGACTTTTTCCGGCGGCT
GCTCATGGAAGATGGGATGCAGCGGAACCTGAAGATGGAGATGCTTGCCGGGTGTGGGGC
TGGGATGTGCCAGGTCGTGGTGACCTGTCCCATGGAAATGCTCAAGATTCAGCTGCAGGA
TGCTGGACGCCTGGCCGTCCATCATCAGGGCTCGGCCTCAGCACCCTCCACCTCCAGGTC
CTACACAACTGGTTCGGCTTCCACCCACAGGCGCCCCTCTGCCACCCTCATTGCCTGGGA
GCTGCTCCGCACTCAGGGCCTGGCTGGGCTCTACAGGGGCCTGGGTGCCACTCTCCTCAG
AGACATTCCTTTCTCCATCATCTACTTCCCACTGTTTGCCAACCTTAACAACCTGGGGTT
CAACGAGCTCGCCGGTAAGGCGTCCTTTGCACATTCCTTCGTGTCAGGCTGTGTGGCAGG
TTCCATAGCTGCGGTCGCAGTGACGCCTCTAGATGTTCTGAAAACTCGAATCCAAACCCT
CAAGAAAGGCCTGGGCGAGGACATGTACAGTGGGATCACCGACTGTGCCAGGAAACTCTG
GATTCAGGAGGGACCATCTGCCTTCATGAAAGGCGCTGGCTGCCGGGCACTGGTCATAGC
ACCTCTCTTTGGGATTGCTCAAGGGGTCTATTTTATTGGGATTGGAGAGCGCATCTTAAA
GTGTTTTGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT