PJA1 ORF clone FHC06375

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC06375

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Clone info.

Species

human

Clone Name

pFN21AB5190 Sequence (with vector) Restriction Map

Product ID

FHC06375

Flexi Clone PID

FXC06375

Original Clone PID

ORK06375

ORF Sequence

DNA sequence (1764 bp)
Protein sequence (588 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527200]

Gene

KIBB5190

Symbol

PJA1

Alias : PRAJA1, RNF70

Description

praja ring finger 1, E3 ubiquitin protein ligase, transcript variant 2

Original Clone ID

bm01524

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1764 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 588 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS35316.1 4.4e-162 100.0 PJA1 CCDS14393.1 4.7e-162 100.0 PJA1 CCDS14392.1 2.5e-97 98.6 PJA1 CCDS4099.1 1.8e-44 46.9 PJA2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018957 540 580 PF00097 Zinc finger HMMSmart IPR001841 540 580 SM00184 Zinc finger ProfileScan IPR001841 540 581 PS50089 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001027568 588 300420 100.0 100.0 XP_005262349 588 300420 100.0 100.0 XP_011529313 643 300420 100.0 100.0 NP_660095 643 300420 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5190 Download

>KIBB5190  1764 bp
ATGCACAGATCAGCCCCCAGTCAAACCACCAAGAGGAGCCGATCGCCATTTTCCACTACT
CGTCGTAGTTGGGACGACAGCGAGAGTTCGGGAACCAACCTGAATATTGATAATGAGGAC
TATTCCAGGTATCCGCCAAGAGAGTACAGAGCTTCGGGTAGCAGAAGAGGAATGGCCTAT
GGACATATTGACTCTTATGGGGCAGATGATAGTGAGGAGGAGGGGGCTGGGCCTGTTGAG
CGACCGCCAGTGAGAGGGAAAACTGGCAAGTTTAAAGATGATAAGCTGTATGACCCAGAG
AAAGGGGCAAGGTCTTTGGCTGGGCCACCTCCACATTTCTCTAGTTTTAGCCGTGATGTG
AGAGAGGAGCGAGACAAGTTAGACCCAGTCCCTGCAGCAAGATGCTCAGCTAGCAGAGCT
GACTTCCTGCCACAAAGTAGTGTGGCCTCACAGTCGTCTTCTGAAGGCAAGCTGGCTACA
AAAGGTGACAGCTCGGAGAGGGAGAGAAGGGAGCAAAATTTACCTGCACGTCCCAGCAGG
GCTCCTGTGAGTATTTGTGGTGGTGGGGAAAACACCTCAAAGAGTGCAGAGGAACCTGTG
GTCAGGCCCAAAATCAGAAACCTGGCAAGTCCAAACTGCGTGAAACCAAAAATTTTTTTT
GATACTGATGATGATGACGATATGCCACACAGTACTTCCAGGTGGAGGGATACCGCCAAT
GACAATGAGGGCCACTCGGATGGCCTGGCAAGAAGAGGGAGAGGCGAGAGTTCAAGTGGC
TATCCCGAGCCAAAGTACCCTGAAGACAAACGGGAAGCGAGGAGTGACCAAGTGAAACCA
GAAAAGGTGCCGAGACGACGACGCACCATGGCCGACCCTGACTTCTGGACGCACAGTGAT
GATTACTACAAATACTGCGACGAAGACTCTGACAGTGACAAAGAGTGGATTGCTGCTCTG
CGTCGGAAATATCGAAGCCGAGAGCAAACCCTGTCCTCCAGTGGCGAAAGCTGGGAGACT
CTGCCGGGGAAAGAAGAGCGGGAACCTCCACAGGCTAAGGTGAGTGCCAGCACTGGCACC
AGCCCTGGCCCCGGTGCTAGTGCCAGTGCCGGGGCTGGCGCCGGGGCCAGTGCTGGCAGC
AATGGCAGCAATTACCTTGAAGAAGTTCGAGAACCATCTCTTCAGGAAGAGCAGGCATCC
CTGGAAGAAGGAGAAATTCCTTGGCTCCAGTACCATGAGAATGACAGTAGCAGTGAGGGG
GATAATGATTCTGGTCACGAGTTGATGCAACCTGGGGTATTCATGCTGGATGGAAACAAC
AACCTTGAAGATGACTCCAGTGTGAGCGAAGACCTAGAAGTGGATTGGAGCCTCTTTGAT
GGATTTGCAGATGGGTTAGGAGTGGCTGAAGCCATTTCCTATGTGGACCCTCAGTTCCTC
ACCTACATGGCACTTGAAGAACGCCTGGCCCAGGCAATGGAAACTGCCCTTGCGCACTTG
GAGTCTCTCGCAGTGGATGTAGAGGTGGCCAATCCACCAGCAAGCAAGGAGAGCATTGAC
GCTCTTCCCGAGATCCTGGTCACTGAAGATCATGGCGCAGTTGGTCAGGAGATGTGCTGC
CCCATCTGCTGTAGCGAATATGTGAAGGGGGAGGTGGCAACTGAGCTGCCGTGCCACCAC
TATTTCCACAAGCCGTGTGTGTCCATCTGGCTTCAGAAGTCAGGCACCTGCCCCGTGTGC
CGCTGCATGTTCCCTCCCCCACTC

ORF Protein

Cloned ORF protein sequence for pFN21AB5190 Download

>KIBB5190  588 aa
MHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAY
GHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDV
REERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGDSSERERREQNLPARPSR
APVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTAN
DNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSD
DYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGT
SPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEG
DNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFL
TYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCC
PICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5190 Download

>pFN21AB5190  6469 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCACAGATCAGCCCCCAGTCAAACCACCAAGAG
GAGCCGATCGCCATTTTCCACTACTCGTCGTAGTTGGGACGACAGCGAGAGTTCGGGAAC
CAACCTGAATATTGATAATGAGGACTATTCCAGGTATCCGCCAAGAGAGTACAGAGCTTC
GGGTAGCAGAAGAGGAATGGCCTATGGACATATTGACTCTTATGGGGCAGATGATAGTGA
GGAGGAGGGGGCTGGGCCTGTTGAGCGACCGCCAGTGAGAGGGAAAACTGGCAAGTTTAA
AGATGATAAGCTGTATGACCCAGAGAAAGGGGCAAGGTCTTTGGCTGGGCCACCTCCACA
TTTCTCTAGTTTTAGCCGTGATGTGAGAGAGGAGCGAGACAAGTTAGACCCAGTCCCTGC
AGCAAGATGCTCAGCTAGCAGAGCTGACTTCCTGCCACAAAGTAGTGTGGCCTCACAGTC
GTCTTCTGAAGGCAAGCTGGCTACAAAAGGTGACAGCTCGGAGAGGGAGAGAAGGGAGCA
AAATTTACCTGCACGTCCCAGCAGGGCTCCTGTGAGTATTTGTGGTGGTGGGGAAAACAC
CTCAAAGAGTGCAGAGGAACCTGTGGTCAGGCCCAAAATCAGAAACCTGGCAAGTCCAAA
CTGCGTGAAACCAAAAATTTTTTTTGATACTGATGATGATGACGATATGCCACACAGTAC
TTCCAGGTGGAGGGATACCGCCAATGACAATGAGGGCCACTCGGATGGCCTGGCAAGAAG
AGGGAGAGGCGAGAGTTCAAGTGGCTATCCCGAGCCAAAGTACCCTGAAGACAAACGGGA
AGCGAGGAGTGACCAAGTGAAACCAGAAAAGGTGCCGAGACGACGACGCACCATGGCCGA
CCCTGACTTCTGGACGCACAGTGATGATTACTACAAATACTGCGACGAAGACTCTGACAG
TGACAAAGAGTGGATTGCTGCTCTGCGTCGGAAATATCGAAGCCGAGAGCAAACCCTGTC
CTCCAGTGGCGAAAGCTGGGAGACTCTGCCGGGGAAAGAAGAGCGGGAACCTCCACAGGC
TAAGGTGAGTGCCAGCACTGGCACCAGCCCTGGCCCCGGTGCTAGTGCCAGTGCCGGGGC
TGGCGCCGGGGCCAGTGCTGGCAGCAATGGCAGCAATTACCTTGAAGAAGTTCGAGAACC
ATCTCTTCAGGAAGAGCAGGCATCCCTGGAAGAAGGAGAAATTCCTTGGCTCCAGTACCA
TGAGAATGACAGTAGCAGTGAGGGGGATAATGATTCTGGTCACGAGTTGATGCAACCTGG
GGTATTCATGCTGGATGGAAACAACAACCTTGAAGATGACTCCAGTGTGAGCGAAGACCT
AGAAGTGGATTGGAGCCTCTTTGATGGATTTGCAGATGGGTTAGGAGTGGCTGAAGCCAT
TTCCTATGTGGACCCTCAGTTCCTCACCTACATGGCACTTGAAGAACGCCTGGCCCAGGC
AATGGAAACTGCCCTTGCGCACTTGGAGTCTCTCGCAGTGGATGTAGAGGTGGCCAATCC
ACCAGCAAGCAAGGAGAGCATTGACGCTCTTCCCGAGATCCTGGTCACTGAAGATCATGG
CGCAGTTGGTCAGGAGATGTGCTGCCCCATCTGCTGTAGCGAATATGTGAAGGGGGAGGT
GGCAACTGAGCTGCCGTGCCACCACTATTTCCACAAGCCGTGTGTGTCCATCTGGCTTCA
GAAGTCAGGCACCTGCCCCGTGTGCCGCTGCATGTTCCCTCCCCCACTCGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT