Gene/Protein Characteristic Table for FHC06109

Species
human

Product ID
FHC06109

Corresponding
Flexi Clone PID

ORF Sequence
DNA sequence (579 bp)
Protein sequence (193 aa)

Linker Type
Flexi type
The extra Val codon is attached to the 3'-end of the ORF, because a Pme I site ...

Cloning Site
SgfI-PmeI

GenBank Accession
ORF sequence is derived from [AB528192]

Gene
KIEE0169

Symbol
NCALD

Description
neurocalcin delta

Original Clone ID
oc00869

Quality control data of this clone

Characterization of the cloned ORF

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Features of the cloned ORF sequence

Length: 579 bp
Restriction map A Restriction map B
Features of the protein sequence

Length: 193 aa
Result of homology search against nr database ( FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAH36098 2.7e-81 100.0 Neurocalcin del...
Homo sapiens
NP_776823 5.2e-81 99.5 neurocalcin-del...
Bos taurus
NP_001123413 8.5e-81 99.0 neurocalcin-del...
Xenopus (Silura...
NP_990474 9.9e-81 99.0 neurocalcin-del...
Gallus gallus
XP_003965892 9.9e-81 99.0 neurocalcin-del...
Takifugu rubripes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
[ zoom out (x2) ] PD000012 PR00450 PR00450 PR00450 PR00450 PR00450 PR00450 PR00450 PF00036 PF00036 PF00036 SM00054 SM00054 SM00054 PS50222 PS50222 PS50222 PS50222 PS00018 PS00018 PS00018

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR002048 99 170 PD000012 Calcium-binding EF-hand
FPrintScan IPR001125 8 22 PR00450 Recoverin
IPR001125 22 41 PR00450 Recoverin
IPR001125 68 89 PR00450 Recoverin
IPR001125 92 111 PR00450 Recoverin
IPR001125 114 132 PR00450 Recoverin
IPR001125 138 153 PR00450 Recoverin
IPR001125 164 184 PR00450 Recoverin
HMMPfam IPR002048 64 92 PF00036 Calcium-binding EF-hand
IPR002048 100 128 PF00036 Calcium-binding EF-hand
IPR002048 148 176 PF00036 Calcium-binding EF-hand
HMMSmart IPR002048 64 92 SM00054 Calcium-binding EF-hand
IPR002048 100 128 SM00054 Calcium-binding EF-hand
IPR002048 148 176 SM00054 Calcium-binding EF-hand
ProfileScan IPR002048 40 58 PS50222 Calcium-binding EF-hand
IPR002048 60 95 PS50222 Calcium-binding EF-hand
IPR002048 96 131 PS50222 Calcium-binding EF-hand
IPR002048 144 179 PS50222 Calcium-binding EF-hand
ScanRegExp IPR002048 73 85 PS00018 Calcium-binding EF-hand
IPR002048 109 121 PS00018 Calcium-binding EF-hand
IPR002048 157 169 PS00018 Calcium-binding EF-hand

Result of homology search against disease genes in OMIM database
(FASTA output, Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%)
NP_001035718 193 606722 100.0 100.0
NP_001035719 193 606722 100.0 100.0
NP_001035714 193 606722 100.0 100.0
NP_001035716 193 606722 100.0 100.0
NP_114430 193 606722 100.0 100.0
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