NAGA ORF clone FHC06094

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC06094

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Clone info.

Species

human

Clone Name

pFN21AE0531 Sequence (with vector) Restriction Map

Product ID

FHC06094

Flexi Clone PID

FXC06094

ORF Sequence

DNA sequence (1233 bp)
Protein sequence (411 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528190]

Gene

KIEE0531

Symbol

NAGA

Alias : D22S674, GALB

Description

N-acetylgalactosaminidase, alpha-

Original Clone ID

oc01231

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1233 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 411 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14030.1 3.4e-202 100.0 NAGA CCDS14484.1 1.2e-86 49.9 GLA The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002241 20 39 PR00740 Glycoside hydrolase IPR002241 66 81 PR00740 Glycoside hydrolase IPR002241 106 127 PR00740 Glycoside hydrolase IPR002241 141 158 PR00740 Glycoside hydrolase IPR002241 168 186 PR00740 Glycoside hydrolase IPR002241 243 262 PR00740 Glycoside hydrolase IPR002241 264 285 PR00740 Glycoside hydrolase HMMPfam IPR000111 24 132 PF02065 Glycoside hydrolase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MLLKTVLLLGHVAQVLMLDNGLL 23 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005261672 411 104170,609241,609242 100.0 100.0 XP_005261673 411 104170,609241,609242 100.0 100.0 NP_000253 411 104170,609241,609242 100.0 100.0 NP_000160 429 300644,301500 49.9 95.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0531 Download

>KIEE0531  1233 bp
ATGCTGCTGAAGACAGTGCTCTTGCTGGGACATGTGGCCCAGGTGCTGATGCTGGACAAT
GGGCTCCTGCAGACACCACCCATGGGCTGGCTGGCCTGGGAACGCTTCCGCTGCAACATT
AACTGTGATGAGGACCCAAAGAACTGCATAAGTGAACAGCTCTTCATGGAGATGGCTGAC
CGGATGGCACAGGATGGATGGCGGGACATGGGCTACACATACCTCAACATTGATGACTGC
TGGATCGGTGGTCGCGATGCCAGTGGCCGCCTGATGCCGGATCCCAAGCGCTTCCCTCAT
GGCATTCCTTTCCTGGCTGACTACGTTCACTCCCTGGGCCTGAAGTTGGGTATCTACGCG
GACATGGGCAACTTCACCTGCATGGGTTACCCAGGCACCACACTGGACAAGGTGGTCCAG
GATGCTCAGACCTTCGCCGAGTGGAAGGTAGACATGCTCAAGCTGGATGGCTGCTTCTCC
ACCCCCGAGGAGCGGGCCCAGGGGTACCCCAAGATGGCTGCTGCCCTGAATGCCACAGGC
CGCCCCATCGCCTTCTCCTGCAGCTGGCCAGCCTATGAAGGCGGCCTCCCCCCAAGGGTG
AACTACAGTCTGCTGGCGGACATCTGCAACCTCTGGCGTAACTATGATGACATCCAGGAC
TCCTGGTGGAGCGTGCTCTCCATCCTGAATTGGTTCGTGGAGCACCAGGACATACTGCAG
CCAGTGGCCGGCCCTGGGCACTGGAATGACCCTGACATGCTGCTCATTGGGAACTTTGGT
CTCAGCTTAGAGCAATCCCGGGCCCAGATGGCCCTGTGGACGGTGCTGGCAGCCCCCCTC
TTGATGTCCACAGACCTGCGTACCATCTCCGCCCAGAACATGGACATTCTGCAGAATCCA
CTCATGATCAAAATCAACCAGGATCCCTTAGGCATCCAGGGACGCAGGATTCACAAGGAA
AAATCTCTCATCGAAGTGTACATGCGGCCTCTGTCCAACAAGGCTAGCGCCTTAGTCTTC
TTCAGCTGCAGGACCGATATGCCTTATCGCTACCACTCCTCCCTTGGCCAGCTGAACTTC
ACCGGGTCTGTGATATATGAGGCCCAGGACGTCTACTCAGGTGACATCATCAGTGGCCTC
CGAGATGAAACCAACTTCACAGTGATCATCAACCCTTCAGGGGTAGTGATGTGGTACCTG
TATCCCATCAAGAACCTGGAGATGTCCCAGCAG

ORF Protein

Cloned ORF protein sequence for pFN21AE0531 Download

>KIEE0531  411 aa
MLLKTVLLLGHVAQVLMLDNGLLQTPPMGWLAWERFRCNINCDEDPKNCISEQLFMEMAD
RMAQDGWRDMGYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLGIYA
DMGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFSTPEERAQGYPKMAAALNATG
RPIAFSCSWPAYEGGLPPRVNYSLLADICNLWRNYDDIQDSWWSVLSILNWFVEHQDILQ
PVAGPGHWNDPDMLLIGNFGLSLEQSRAQMALWTVLAAPLLMSTDLRTISAQNMDILQNP
LMIKINQDPLGIQGRRIHKEKSLIEVYMRPLSNKASALVFFSCRTDMPYRYHSSLGQLNF
TGSVIYEAQDVYSGDIISGLRDETNFTVIINPSGVVMWYLYPIKNLEMSQQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0531 Download

>pFN21AE0531  5938 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTGCTGAAGACAGTGCTCTTGCTGGGACATGT
GGCCCAGGTGCTGATGCTGGACAATGGGCTCCTGCAGACACCACCCATGGGCTGGCTGGC
CTGGGAACGCTTCCGCTGCAACATTAACTGTGATGAGGACCCAAAGAACTGCATAAGTGA
ACAGCTCTTCATGGAGATGGCTGACCGGATGGCACAGGATGGATGGCGGGACATGGGCTA
CACATACCTCAACATTGATGACTGCTGGATCGGTGGTCGCGATGCCAGTGGCCGCCTGAT
GCCGGATCCCAAGCGCTTCCCTCATGGCATTCCTTTCCTGGCTGACTACGTTCACTCCCT
GGGCCTGAAGTTGGGTATCTACGCGGACATGGGCAACTTCACCTGCATGGGTTACCCAGG
CACCACACTGGACAAGGTGGTCCAGGATGCTCAGACCTTCGCCGAGTGGAAGGTAGACAT
GCTCAAGCTGGATGGCTGCTTCTCCACCCCCGAGGAGCGGGCCCAGGGGTACCCCAAGAT
GGCTGCTGCCCTGAATGCCACAGGCCGCCCCATCGCCTTCTCCTGCAGCTGGCCAGCCTA
TGAAGGCGGCCTCCCCCCAAGGGTGAACTACAGTCTGCTGGCGGACATCTGCAACCTCTG
GCGTAACTATGATGACATCCAGGACTCCTGGTGGAGCGTGCTCTCCATCCTGAATTGGTT
CGTGGAGCACCAGGACATACTGCAGCCAGTGGCCGGCCCTGGGCACTGGAATGACCCTGA
CATGCTGCTCATTGGGAACTTTGGTCTCAGCTTAGAGCAATCCCGGGCCCAGATGGCCCT
GTGGACGGTGCTGGCAGCCCCCCTCTTGATGTCCACAGACCTGCGTACCATCTCCGCCCA
GAACATGGACATTCTGCAGAATCCACTCATGATCAAAATCAACCAGGATCCCTTAGGCAT
CCAGGGACGCAGGATTCACAAGGAAAAATCTCTCATCGAAGTGTACATGCGGCCTCTGTC
CAACAAGGCTAGCGCCTTAGTCTTCTTCAGCTGCAGGACCGATATGCCTTATCGCTACCA
CTCCTCCCTTGGCCAGCTGAACTTCACCGGGTCTGTGATATATGAGGCCCAGGACGTCTA
CTCAGGTGACATCATCAGTGGCCTCCGAGATGAAACCAACTTCACAGTGATCATCAACCC
TTCAGGGGTAGTGATGTGGTACCTGTATCCCATCAAGAACCTGGAGATGTCCCAGCAGGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT