LIMS1 ORF clone FHC05836

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC05836

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Clone info.

Species

human

Clone Name

pFN21AB6473 Sequence (with vector) Restriction Map

Product ID

FHC05836

Flexi Clone PID

FXC05836

ORF Sequence

DNA sequence (975 bp)
Protein sequence (325 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463831]

Gene

KIBB6473

Symbol

LIMS1

Alias : PINCH, PINCH-1, PINCH1

Description

LIM and senescent cell antigen-like domains 1, transcript variant 2

Original Clone ID

cs01073

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 975 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 325 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2078.1 3.4e-88 100.0 LIMS1 CCDS54383.1 3.4e-88 100.0 LIMS1 CCDS54382.1 3.4e-88 100.0 LIMS1 CCDS54384.1 3.6e-88 100.0 LIMS1 CCDS54385.1 3.8e-88 100.0 LIMS1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001781 10 66 PF00412 Zinc finger IPR001781 71 125 PF00412 Zinc finger IPR001781 135 186 PF00412 Zinc finger IPR001781 193 247 PF00412 Zinc finger IPR001781 252 306 PF00412 Zinc finger HMMSmart IPR001781 9 62 SM00132 Zinc finger IPR001781 70 121 SM00132 Zinc finger IPR001781 134 184 SM00132 Zinc finger IPR001781 192 243 SM00132 Zinc finger IPR001781 251 303 SM00132 Zinc finger ProfileScan IPR001781 8 69 PS50023 Zinc finger IPR001781 71 128 PS50023 Zinc finger IPR001781 133 190 PS50023 Zinc finger IPR001781 191 250 PS50023 Zinc finger IPR001781 251 310 PS50023 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001180417 325 602567 100.0 100.0 NP_004978 325 602567 100.0 100.0 NP_001180411 329 602567 100.0 100.0 NP_001180412 337 602567 100.0 100.0 NP_001180413 362 602567 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6473 Download

>KIBB6473  975 bp
ATGGCCAACGCCCTGGCCAGCGCCACTTGCGAGCGCTGCAAGGGCGGCTTTGCGCCCGCT
GAGAAGATCGTGAACAGTAATGGGGAGCTGTACCATGAGCAGTGTTTCGTGTGCGCTCAG
TGCTTCCAGCAGTTCCCAGAAGGACTCTTCTATGAGTTTGAAGGAAGAAAGTACTGTGAA
CATGACTTTCAGATGCTCTTTGCCCCTTGCTGTCATCAGTGTGGTGAATTCATCATTGGC
CGAGTTATCAAAGCCATGAATAACAGCTGGCATCCGGAGTGCTTCCGCTGTGACCTCTGC
CAGGAAGTTCTGGCAGATATCGGGTTTGTCAAGAATGCTGGGAGACACCTGTGTCGCCCC
TGTCATAATCGTGAGAAAGCCAGAGGCCTTGGGAAATACATCTGCCAGAAATGCCATGCT
ATCATCGATGAGCAGCCTCTGATATTCAAGAACGACCCCTACCATCCAGACCATTTCAAC
TGCGCCAACTGCGGGAAGGAGCTGACTGCCGATGCACGGGAGCTGAAAGGGGAGCTATAC
TGCCTCCCATGCCATGATAAAATGGGGGTCCCCATCTGTGGTGCTTGCCGACGGCCCATC
GAAGGGCGCGTGGTGAACGCTATGGGCAAGCAGTGGCATGTGGAGCATTTTGTTTGTGCC
AAGTGTGAGAAACCCTTTCTTGGACATCGCCATTATGAGAGGAAAGGCCTGGCATATTGT
GAAACTCACTATAACCAGCTATTTGGTGATGTTTGCTTCCACTGCAATCGTGTTATAGAA
GGTGATGTGGTCTCTGCTCTTAATAAGGCCTGGTGCGTGAACTGCTTTGCCTGTTCTACC
TGCAACACTAAATTAACACTCAAGAATAAGTTTGTGGAGTTTGACATGAAGCCAGTCTGT
AAGAAGTGCTATGAGAAATTTCCATTGGAGCTGAAGAAAAGACTTAAGAAACTAGCTGAG
ACCTTAGGAAGGAAA

ORF Protein

Cloned ORF protein sequence for pFN21AB6473 Download

>KIBB6473  325 aa
MANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCE
HDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRP
CHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELY
CLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYC
ETHYNQLFGDVCFHCNRVIEGDVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVC
KKCYEKFPLELKKRLKKLAETLGRK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6473 Download

>pFN21AB6473  5680 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCAACGCCCTGGCCAGCGCCACTTGCGAGCG
CTGCAAGGGCGGCTTTGCGCCCGCTGAGAAGATCGTGAACAGTAATGGGGAGCTGTACCA
TGAGCAGTGTTTCGTGTGCGCTCAGTGCTTCCAGCAGTTCCCAGAAGGACTCTTCTATGA
GTTTGAAGGAAGAAAGTACTGTGAACATGACTTTCAGATGCTCTTTGCCCCTTGCTGTCA
TCAGTGTGGTGAATTCATCATTGGCCGAGTTATCAAAGCCATGAATAACAGCTGGCATCC
GGAGTGCTTCCGCTGTGACCTCTGCCAGGAAGTTCTGGCAGATATCGGGTTTGTCAAGAA
TGCTGGGAGACACCTGTGTCGCCCCTGTCATAATCGTGAGAAAGCCAGAGGCCTTGGGAA
ATACATCTGCCAGAAATGCCATGCTATCATCGATGAGCAGCCTCTGATATTCAAGAACGA
CCCCTACCATCCAGACCATTTCAACTGCGCCAACTGCGGGAAGGAGCTGACTGCCGATGC
ACGGGAGCTGAAAGGGGAGCTATACTGCCTCCCATGCCATGATAAAATGGGGGTCCCCAT
CTGTGGTGCTTGCCGACGGCCCATCGAAGGGCGCGTGGTGAACGCTATGGGCAAGCAGTG
GCATGTGGAGCATTTTGTTTGTGCCAAGTGTGAGAAACCCTTTCTTGGACATCGCCATTA
TGAGAGGAAAGGCCTGGCATATTGTGAAACTCACTATAACCAGCTATTTGGTGATGTTTG
CTTCCACTGCAATCGTGTTATAGAAGGTGATGTGGTCTCTGCTCTTAATAAGGCCTGGTG
CGTGAACTGCTTTGCCTGTTCTACCTGCAACACTAAATTAACACTCAAGAATAAGTTTGT
GGAGTTTGACATGAAGCCAGTCTGTAAGAAGTGCTATGAGAAATTTCCATTGGAGCTGAA
GAAAAGACTTAAGAAACTAGCTGAGACCTTAGGAAGGAAAGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT