KLHL18 ORF clone FHC05762

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC05762

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Clone info.

Species

human

Clone Name

pFN21AE0309 Sequence (with vector) Restriction Map

Product ID

FHC05762

Flexi Clone PID

FXC05762

ORF Sequence

DNA sequence (1722 bp)
Protein sequence (574 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527175]

Gene

KIEE0309

Symbol

KLHL18

Description

kelch-like family member 18

Original Clone ID

oc01009

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1722 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 574 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33749.1 0 100.0 KLHL18 CCDS1310.1 9.1e-97 42.9 KLHL20 CCDS30550.1 9.5e-97 43.2 KLHL17 CCDS1429.1 2e-88 41.7 KLHL12 CCDS54756.1 2.4e-88 41.1 KLHL5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 28 134 PF00651 BTB/POZ IPR011705 140 242 PF07707 BTB/Kelch-associated IPR006652 282 322 PF01344 Kelch repeat type 1 IPR006652 325 370 PF01344 Kelch repeat type 1 IPR006652 373 417 PF01344 Kelch repeat type 1 IPR006652 420 463 PF01344 Kelch repeat type 1 IPR006652 467 510 PF01344 Kelch repeat type 1 IPR006652 513 557 PF01344 Kelch repeat type 1 HMMSmart IPR000210 38 135 SM00225 BTB/POZ-like IPR011705 140 242 SM00875 BTB/Kelch-associated IPR006652 289 336 SM00612 Kelch repeat type 1 IPR006652 337 383 SM00612 Kelch repeat type 1 IPR006652 384 430 SM00612 Kelch repeat type 1 IPR006652 431 477 SM00612 Kelch repeat type 1 IPR006652 478 524 SM00612 Kelch repeat type 1 IPR006652 525 571 SM00612 Kelch repeat type 1 ProfileScan IPR000210 38 105 PS50097 BTB/POZ-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_067646 568 614522 41.7 94.4 NP_001289980 606 614522 41.7 94.4 XP_011508137 623 614522 41.7 94.4 NP_001165125 568 608064 41.1 94.8 NP_001007076 709 608064 41.1 94.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0309 Download

>KIEE0309  1722 bp
ATGGTGGAGGACGGCGCGGAGGAGCTGGAGGATCTGGTGCACTTCTCCGTGTCTGAGTTG
CCTAGTCGCGGCTACGGCGTCATGGAGGAGATCCGGCGGCAGGGCAAGCTGTGCGACGTG
ACCCTCAAGATTGGGGACCACAAATTCAGTGCCCACCGGATTGTCTTGGCAGCCTCGATC
CCGTATTTCCATGCTATGTTTACAAATGACATGATGGAGTGCAAGCAGGATGAGATTGTA
ATGCAAGGAATGGACCCAAGTGCCCTGGAGGCTCTGATCAACTTTGCCTACAACGGCAAC
CTTGCCATTGACCAGCAAAATGTCCAGTCATTGCTGATGGGGGCGAGCTTCCTGCAGCTG
CAGAGCATCAAAGACGCCTGCTGCACATTCCTTCGAGAACGGCTTCACCCAAAAAACTGC
CTGGGTGTGCGCCAGTTTGCTGAGACAATGATGTGTGCTGTGCTGTACGACGCTGCCAAC
AGCTTCATCCACCAGCACTTTGTGGAGGTGTCCATGTCAGAAGAGTTCCTGGCCCTGCCC
TTGGAAGACGTGCTTGAGCTGGTGTCTCGGGATGAGCTGAATGTCAAATCTGAGGAGCAG
GTCTTTGAAGCTGCATTGGCCTGGGTCAGATACGACCGGGAGCAGAGGGGTCCCTACCTG
CCTGAGCTGCTGTCCAATATCCGCCTGCCCCTCTGTCGGCCCCAGTTCCTTTCAGACAGA
GTACAGCAGGATGACCTGGTGCGTTGCTGCCACAAATGCAGGGACCTGGTAGACGAAGCA
AAGGACTACCACCTCATGCCAGAGCGCCGGCCCCACCTGCCAGCTTTCAGAACCCGGCCA
CGCTGCTGCACATCCATCGCTGGACTTATCTACGCTGTAGGGGGCCTCAACTCAGCAGGT
GATTCCCTGAATGTGGTGGAAGTGTTCGACCCCATTGCCAATTGCTGGGAGAGATGCCGT
CCCATGACAACAGCCCGCAGCCGCGTTGGCGTGGCTGTGGTGAACGGGCTTCTCTATGCC
ATCGGAGGATATGACGGCCAGCTACGGCTGAGCACTGTGGAGGCCTACAACCCGGAGACA
GACACATGGACCAGAGTGGGGAGCATGAATAGCAAGAGAAGTGCCATGGGGACAGTCGTG
CTGGATGGGCAGATCTACGTCTGTGGGGGCTACGATGGCAACTCTTCCCTCAGCTCCGTG
GAGACCTACTCACCTGAGACGGACAAATGGACAGTGGTGACCTCGATGAGCTCGAATCGC
AGTGCTGCTGGGGTTACAGTCTTTGAGGGCAGGATATATGTGTCAGGCGGCCATGATGGT
TTGCAGATCTTCAGCAGTGTGGAACACTACAACCACCACACAGCCACCTGGCACCCTGCA
GCTGGCATGCTCAACAAGCGCTGCCGGCACGGAGCCGCCTCCCTGGGGAGCAAGATGTTT
GTCTGCGGGGGCTACGATGGCTCTGGCTTCCTCAGCATTGCCGAGATGTACAGCTCTGTG
GCAGACCAGTGGTGCCTGATTGTCCCCATGCACACGCGCAGGAGCCGGGTCTCCCTGGTG
GCCAGCTGTGGGCGCCTCTACGCTGTTGGGGGCTACGACGGACAGTCAAACCTAAGCTCA
GTGGAGATGTATGACCCAGAGACAGACTGCTGGACATTCATGGCCCCCATGGCGTGCCAT
GAGGGAGGGGTCGGTGTGGGCTGCATCCCTCTCCTCACCATC

ORF Protein

Cloned ORF protein sequence for pFN21AE0309 Download

>KIEE0309  574 aa
MVEDGAEELEDLVHFSVSELPSRGYGVMEEIRRQGKLCDVTLKIGDHKFSAHRIVLAASI
PYFHAMFTNDMMECKQDEIVMQGMDPSALEALINFAYNGNLAIDQQNVQSLLMGASFLQL
QSIKDACCTFLRERLHPKNCLGVRQFAETMMCAVLYDAANSFIHQHFVEVSMSEEFLALP
LEDVLELVSRDELNVKSEEQVFEAALAWVRYDREQRGPYLPELLSNIRLPLCRPQFLSDR
VQQDDLVRCCHKCRDLVDEAKDYHLMPERRPHLPAFRTRPRCCTSIAGLIYAVGGLNSAG
DSLNVVEVFDPIANCWERCRPMTTARSRVGVAVVNGLLYAIGGYDGQLRLSTVEAYNPET
DTWTRVGSMNSKRSAMGTVVLDGQIYVCGGYDGNSSLSSVETYSPETDKWTVVTSMSSNR
SAAGVTVFEGRIYVSGGHDGLQIFSSVEHYNHHTATWHPAAGMLNKRCRHGAASLGSKMF
VCGGYDGSGFLSIAEMYSSVADQWCLIVPMHTRRSRVSLVASCGRLYAVGGYDGQSNLSS
VEMYDPETDCWTFMAPMACHEGGVGVGCIPLLTI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0309 Download

>pFN21AE0309  6427 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTGGAGGACGGCGCGGAGGAGCTGGAGGATCT
GGTGCACTTCTCCGTGTCTGAGTTGCCTAGTCGCGGCTACGGCGTCATGGAGGAGATCCG
GCGGCAGGGCAAGCTGTGCGACGTGACCCTCAAGATTGGGGACCACAAATTCAGTGCCCA
CCGGATTGTCTTGGCAGCCTCGATCCCGTATTTCCATGCTATGTTTACAAATGACATGAT
GGAGTGCAAGCAGGATGAGATTGTAATGCAAGGAATGGACCCAAGTGCCCTGGAGGCTCT
GATCAACTTTGCCTACAACGGCAACCTTGCCATTGACCAGCAAAATGTCCAGTCATTGCT
GATGGGGGCGAGCTTCCTGCAGCTGCAGAGCATCAAAGACGCCTGCTGCACATTCCTTCG
AGAACGGCTTCACCCAAAAAACTGCCTGGGTGTGCGCCAGTTTGCTGAGACAATGATGTG
TGCTGTGCTGTACGACGCTGCCAACAGCTTCATCCACCAGCACTTTGTGGAGGTGTCCAT
GTCAGAAGAGTTCCTGGCCCTGCCCTTGGAAGACGTGCTTGAGCTGGTGTCTCGGGATGA
GCTGAATGTCAAATCTGAGGAGCAGGTCTTTGAAGCTGCATTGGCCTGGGTCAGATACGA
CCGGGAGCAGAGGGGTCCCTACCTGCCTGAGCTGCTGTCCAATATCCGCCTGCCCCTCTG
TCGGCCCCAGTTCCTTTCAGACAGAGTACAGCAGGATGACCTGGTGCGTTGCTGCCACAA
ATGCAGGGACCTGGTAGACGAAGCAAAGGACTACCACCTCATGCCAGAGCGCCGGCCCCA
CCTGCCAGCTTTCAGAACCCGGCCACGCTGCTGCACATCCATCGCTGGACTTATCTACGC
TGTAGGGGGCCTCAACTCAGCAGGTGATTCCCTGAATGTGGTGGAAGTGTTCGACCCCAT
TGCCAATTGCTGGGAGAGATGCCGTCCCATGACAACAGCCCGCAGCCGCGTTGGCGTGGC
TGTGGTGAACGGGCTTCTCTATGCCATCGGAGGATATGACGGCCAGCTACGGCTGAGCAC
TGTGGAGGCCTACAACCCGGAGACAGACACATGGACCAGAGTGGGGAGCATGAATAGCAA
GAGAAGTGCCATGGGGACAGTCGTGCTGGATGGGCAGATCTACGTCTGTGGGGGCTACGA
TGGCAACTCTTCCCTCAGCTCCGTGGAGACCTACTCACCTGAGACGGACAAATGGACAGT
GGTGACCTCGATGAGCTCGAATCGCAGTGCTGCTGGGGTTACAGTCTTTGAGGGCAGGAT
ATATGTGTCAGGCGGCCATGATGGTTTGCAGATCTTCAGCAGTGTGGAACACTACAACCA
CCACACAGCCACCTGGCACCCTGCAGCTGGCATGCTCAACAAGCGCTGCCGGCACGGAGC
CGCCTCCCTGGGGAGCAAGATGTTTGTCTGCGGGGGCTACGATGGCTCTGGCTTCCTCAG
CATTGCCGAGATGTACAGCTCTGTGGCAGACCAGTGGTGCCTGATTGTCCCCATGCACAC
GCGCAGGAGCCGGGTCTCCCTGGTGGCCAGCTGTGGGCGCCTCTACGCTGTTGGGGGCTA
CGACGGACAGTCAAACCTAAGCTCAGTGGAGATGTATGACCCAGAGACAGACTGCTGGAC
ATTCATGGCCCCCATGGCGTGCCATGAGGGAGGGGTCGGTGTGGGCTGCATCCCTCTCCT
CACCATCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT