PPM1J ORF clone FHC03730

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03730

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Clone info.

Species

human

Clone Name

pFN21AB5923 Sequence (with vector) Restriction Map

Product ID

FHC03730

Flexi Clone PID

FXC03730

ORF Sequence

DNA sequence (1515 bp)
Protein sequence (505 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527803]

Gene

KIBB5923

Symbol

PPM1J

Alias : PP2C-zeta, PP2CZ, PP2Czeta, PPP2CZ

Description

protein phosphatase, Mg2+/Mn2+ dependent, 1J

Original Clone ID

cs00623

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1515 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 505 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS855.2 3e-127 99.8 PPM1J CCDS44934.1 1.9e-44 55.7 PPM1H CCDS46840.1 1.3e-26 51.2 PPM1M The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001932 218 308 PF00481 Protein phosphatase 2C-like IPR001932 370 453 PF00481 Protein phosphatase 2C-like HMMSmart IPR001932 94 496 SM00332 Protein phosphatase 2C-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 257 EGGCCALVVIYLLGKVYVANAG 278 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005158 505 609957 99.8 100.0 NP_065751 514 616016 55.7 89.9 NP_653242 459 608979 44.1 93.1 XP_005264936 448 608979 45.0 83.4 XP_005264937 374 608979 38.1 93.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5923 Download

>KIBB5923  1515 bp
ATGCTAAACCGGGTGCGCTCGGCCGTGGCGCACCTGGTGAGCTCCGGGGGCGCTCCGCCT
CCGCGCCCCAAATCCCCGGACCTGCCCAACGCCGCCTCGGCGCCGCCCGCCGCCGCTCCA
GAAGCGCCCAGGAGCCCTCCCGCGAAGGCTGGGAGCGGGAGCGCGACGCCCGCGAAGGCT
GTTGAGGCTCGAGCGAGCTTCTCCAGACCGACCTTTCTGCAGCTGAGCCCCGGGGGGCTG
CGACGCGCCGATGACCACGCGGGCCGGGCTGTGCAAAGCCCCCCGGACACGGGCCGCCGC
CTGCCCTGGAGCACAGGCTACGCCGAGGTCATCAATGCTGGCAAGAGTCGGCACAATGAG
GACCAGGCTTGCTGTGAAGTGGTGTATGTGGAAGGTCGGAGGAGTGTTACAGGAGTACCT
AGGGAGCCTAGCCGAGGCCAGGGACTCTGCTTCTACTACTGGGGCCTATTTGATGGGCAT
GCAGGGGGCGGAGCTGCTGAAATGGCCTCACGGCTCCTGCATCGCCATATCCGAGAGCAG
CTAAAGGACCTGGTAGAGATACTTCAGGACCCTTCGCCACCACCCCTCTGCCTCCCAACC
ACTCCGGGGACCCCAGATTCCTCCGATCCCTCTCACTTGCTTGGCCCTCAGTCCTGCTGG
TCTTCACAGAAGGAAGTGAGCCACGAGAGCCTGGTAGTGGGGGCCATTGAGAATGCCTTC
CAGCTCATGGATGAGCAGATGGCCCGGGAGCGGCGTGGCCACCAAGTGGAGGGGGGCTGC
TGTGCACTGGTTGTGATCTACCTGCTAGGCAAGGTGTACGTGGCCAATGCAGGCGATAGC
AGGGCCATCATTGTCCGGAATGGTGAAATCATTCCAATGTCCCGGGAGTTTACCCCGGAG
ACTGAGCGCCAGCGTCTTCAGCTGCTTGGCTTCCTGAAACCAGAGCTGCTAGGCAGTGAA
TTCACCCACCTTGAGTTCCCCCGCAGAGTTCTGCCCAAGGAGCTGGGGCAGAGGATGTTG
TACCGGGACCAGAACATGACCGGCTGGGCCTACAAAAAGATCGAGCTGGAGGATCTCAGG
TTTCCTCTGGTCTGTGGGGAGGGCAAAAAGGCTCGGGTGATGGCCACCATTGGGGTGACC
CGAGGCTTGGGAGACCACAGCCTTAAGGTCTGCAGTTCCACCCTGCCCATCAAGCCCTTT
CTCTCCTGCTTCCCTGAGGTACGAGTGTATGACCTGACACAATATGAGCACTGCCCAGAT
GATGTGCTAGTCCTGGGAACAGATGGCCTGTGGGATGTCACTACTGACTGTGAGGTAGCT
GCCACTGTGGACAGGGTGCTGTCGGCCTATGAGCCTAATGACCACAGCAGGTATACAGCT
CTGGCCCAAGCTCTGGTCCTGGGGGCCCGGGGTACCCCCCGAGACCGTGGCTGGCGTCTC
CCCAACAACAAGCTGGGTTCCGGGGATGACATCTCTGTCTTCGTCATCCCCCTGGGAGGG
CCAGGCAGTTACTCC

ORF Protein

Cloned ORF protein sequence for pFN21AB5923 Download

>KIBB5923  505 aa
MLNRVRSAVAHLVSSGGAPPPRPKSPDLPNAASAPPAAAPEAPRSPPAKAGSGSATPAKA
VEARASFSRPTFLQLSPGGLRRADDHAGRAVQSPPDTGRRLPWSTGYAEVINAGKSRHNE
DQACCEVVYVEGRRSVTGVPREPSRGQGLCFYYWGLFDGHAGGGAAEMASRLLHRHIREQ
LKDLVEILQDPSPPPLCLPTTPGTPDSSDPSHLLGPQSCWSSQKEVSHESLVVGAIENAF
QLMDEQMARERRGHQVEGGCCALVVIYLLGKVYVANAGDSRAIIVRNGEIIPMSREFTPE
TERQRLQLLGFLKPELLGSEFTHLEFPRRVLPKELGQRMLYRDQNMTGWAYKKIELEDLR
FPLVCGEGKKARVMATIGVTRGLGDHSLKVCSSTLPIKPFLSCFPEVRVYDLTQYEHCPD
DVLVLGTDGLWDVTTDCEVAATVDRVLSAYEPNDHSRYTALAQALVLGARGTPRDRGWRL
PNNKLGSGDDISVFVIPLGGPGSYS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5923 Download

>pFN21AB5923  6220 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTAAACCGGGTGCGCTCGGCCGTGGCGCACCT
GGTGAGCTCCGGGGGCGCTCCGCCTCCGCGCCCCAAATCCCCGGACCTGCCCAACGCCGC
CTCGGCGCCGCCCGCCGCCGCTCCAGAAGCGCCCAGGAGCCCTCCCGCGAAGGCTGGGAG
CGGGAGCGCGACGCCCGCGAAGGCTGTTGAGGCTCGAGCGAGCTTCTCCAGACCGACCTT
TCTGCAGCTGAGCCCCGGGGGGCTGCGACGCGCCGATGACCACGCGGGCCGGGCTGTGCA
AAGCCCCCCGGACACGGGCCGCCGCCTGCCCTGGAGCACAGGCTACGCCGAGGTCATCAA
TGCTGGCAAGAGTCGGCACAATGAGGACCAGGCTTGCTGTGAAGTGGTGTATGTGGAAGG
TCGGAGGAGTGTTACAGGAGTACCTAGGGAGCCTAGCCGAGGCCAGGGACTCTGCTTCTA
CTACTGGGGCCTATTTGATGGGCATGCAGGGGGCGGAGCTGCTGAAATGGCCTCACGGCT
CCTGCATCGCCATATCCGAGAGCAGCTAAAGGACCTGGTAGAGATACTTCAGGACCCTTC
GCCACCACCCCTCTGCCTCCCAACCACTCCGGGGACCCCAGATTCCTCCGATCCCTCTCA
CTTGCTTGGCCCTCAGTCCTGCTGGTCTTCACAGAAGGAAGTGAGCCACGAGAGCCTGGT
AGTGGGGGCCATTGAGAATGCCTTCCAGCTCATGGATGAGCAGATGGCCCGGGAGCGGCG
TGGCCACCAAGTGGAGGGGGGCTGCTGTGCACTGGTTGTGATCTACCTGCTAGGCAAGGT
GTACGTGGCCAATGCAGGCGATAGCAGGGCCATCATTGTCCGGAATGGTGAAATCATTCC
AATGTCCCGGGAGTTTACCCCGGAGACTGAGCGCCAGCGTCTTCAGCTGCTTGGCTTCCT
GAAACCAGAGCTGCTAGGCAGTGAATTCACCCACCTTGAGTTCCCCCGCAGAGTTCTGCC
CAAGGAGCTGGGGCAGAGGATGTTGTACCGGGACCAGAACATGACCGGCTGGGCCTACAA
AAAGATCGAGCTGGAGGATCTCAGGTTTCCTCTGGTCTGTGGGGAGGGCAAAAAGGCTCG
GGTGATGGCCACCATTGGGGTGACCCGAGGCTTGGGAGACCACAGCCTTAAGGTCTGCAG
TTCCACCCTGCCCATCAAGCCCTTTCTCTCCTGCTTCCCTGAGGTACGAGTGTATGACCT
GACACAATATGAGCACTGCCCAGATGATGTGCTAGTCCTGGGAACAGATGGCCTGTGGGA
TGTCACTACTGACTGTGAGGTAGCTGCCACTGTGGACAGGGTGCTGTCGGCCTATGAGCC
TAATGACCACAGCAGGTATACAGCTCTGGCCCAAGCTCTGGTCCTGGGGGCCCGGGGTAC
CCCCCGAGACCGTGGCTGGCGTCTCCCCAACAACAAGCTGGGTTCCGGGGATGACATCTC
TGTCTTCGTCATCCCCCTGGGAGGGCCAGGCAGTTACTCCGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT