PPP2R5A ORF clone FHC03428

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03428

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Clone info.

Species

human

Clone Name

pFN21AB5848 Sequence (with vector) Restriction Map

Product ID

FHC03428

Flexi Clone PID

FXC03428

ORF Sequence

DNA sequence (1458 bp)
Protein sequence (486 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527512]

Gene

KIBB5848

Symbol

PPP2R5A

Alias : B56A, B56alpha, PR61A

Description

protein phosphatase 2, regulatory subunit B', alpha, transcript variant 1

Original Clone ID

cs00548

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1458 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 486 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1503.1 1.1e-185 100.0 PPP2R5A CCDS55686.1 2.4e-162 99.8 PPP2R5A CCDS9758.1 2.6e-144 80.0 PPP2R5E CCDS61468.1 2.8e-141 79.2 PPP2R5E CCDS8085.1 7.6e-136 72.0 PPP2R5B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002554 55 461 PF01603 Protein phosphatase 2A
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_006234 486 601643 100.0 100.0 NP_001186685 429 601643 99.8 87.7 NP_001269108 467 601647 80.0 92.8 NP_006237 467 601647 80.0 92.8 NP_001269109 462 601647 79.2 92.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5848 Download

>KIBB5848  1458 bp
ATGTCGTCGTCGTCGCCGCCGGCGGGGGCTGCCAGCGCCGCCATCTCGGCCTCGGAGAAA
GTGGACGGCTTCACCCGGAAATCGGTCCGCAAGGCGCAGAGGCAGAAGCGCTCCCAGGGC
TCGTCGCAGTTTCGCAGCCAGGGCAGCCAGGCAGAGCTGCACCCGCTGCCCCAGCTCAAA
GATGCCACTTCAAATGAACAACAAGAGCTTTTCTGTCAGAAGTTGCAGCAGTGTTGTATA
CTGTTTGATTTCATGGACTCTGTTTCAGACTTGAAGAGCAAAGAAATTAAAAGAGCAACA
CTGAATGAACTGGTTGAGTATGTTTCAACTAATCGTGGTGTAATTGTTGAATCAGCGTAT
TCTGATATAGTAAAAATGATCAGTGCTAACATCTTCCGTACACTTCCTCCAAGTGATAAT
CCAGATTTTGATCCAGAAGAGGATGAACCCACGCTTGAGGCCTCTTGGCCTCACATACAG
TTGGTATATGAATTCTTCTTGAGATTTTTGGAGAGCCCTGATTTCCAGCCTAGCATTGCA
AAACGATACATTGATCAGAAATTCGTACAACAGCTCCTGGAGCTTTTTGATAGTGAAGAT
CCCAGAGAACGTGACTTCCTGAAGACTGTTCTGCACCGAATTTATGGGAAATTTCTTGGA
TTAAGAGCATTCATCAGAAAACAAATTAACAACATTTTCCTCAGGTTTATATATGAAACA
GAACATTTCAATGGTGTTGCTGAACTTCTTGAAATATTAGGAAGTATTATCAATGGCTTT
GCATTGCCACTGAAAGCAGAACATAAACAATTTCTAATGAAGGTTCTTATTCCTATGCAT
ACTGCAAAAGGATTAGCTTTGTTTCATGCTCAGCTAGCATATTGTGTTGTACAGTTCCTG
GAGAAAGATACAACACTAACAGAGCCAGTGATCAGAGGACTGCTGAAATTTTGGCCAAAA
ACCTGCAGTCAGAAAGAGGTGATGTTTTTAGGAGAAATTGAAGAAATCTTAGATGTCATT
GAACCAACACAGTTCAAAAAAATTGAAGAGCCACTTTTCAAGCAGATATCCAAGTGTGTA
TCCAGTTCTCATTTTCAGGTTGCAGAAAGGGCATTGTACTTCTGGAATAACGAATATATT
CTTAGTTTGATTGAGGAGAACATTGATAAAATTCTGCCAATTATGTTTGCCAGTTTGTAC
AAAATTTCCAAAGAACACTGGAATCCGACCATTGTAGCACTGGTATACAATGTGCTGAAA
ACCCTAATGGAAATGAATGGCAAGCTTTTCGATGACCTTACTAGCTCATACAAAGCTGAA
AGACAGAGAGAGAAAAAGAAGGAATTGGAACGTGAAGAATTATGGAAAAAATTAGAGGAG
CTAAAGCTAAAGAAAGCTCTAGAAAAACAGAATAGTGCTTACAACATGCACAGTATTCTC
AGCAATACAAGTGCCGAA

ORF Protein

Cloned ORF protein sequence for pFN21AB5848 Download

>KIBB5848  486 aa
MSSSSPPAGAASAAISASEKVDGFTRKSVRKAQRQKRSQGSSQFRSQGSQAELHPLPQLK
DATSNEQQELFCQKLQQCCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAY
SDIVKMISANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIA
KRYIDQKFVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYET
EHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFL
EKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCV
SSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHWNPTIVALVYNVLK
TLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSIL
SNTSAE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5848 Download

>pFN21AB5848  6163 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGTCGTCGTCGCCGCCGGCGGGGGCTGCCAG
CGCCGCCATCTCGGCCTCGGAGAAAGTGGACGGCTTCACCCGGAAATCGGTCCGCAAGGC
GCAGAGGCAGAAGCGCTCCCAGGGCTCGTCGCAGTTTCGCAGCCAGGGCAGCCAGGCAGA
GCTGCACCCGCTGCCCCAGCTCAAAGATGCCACTTCAAATGAACAACAAGAGCTTTTCTG
TCAGAAGTTGCAGCAGTGTTGTATACTGTTTGATTTCATGGACTCTGTTTCAGACTTGAA
GAGCAAAGAAATTAAAAGAGCAACACTGAATGAACTGGTTGAGTATGTTTCAACTAATCG
TGGTGTAATTGTTGAATCAGCGTATTCTGATATAGTAAAAATGATCAGTGCTAACATCTT
CCGTACACTTCCTCCAAGTGATAATCCAGATTTTGATCCAGAAGAGGATGAACCCACGCT
TGAGGCCTCTTGGCCTCACATACAGTTGGTATATGAATTCTTCTTGAGATTTTTGGAGAG
CCCTGATTTCCAGCCTAGCATTGCAAAACGATACATTGATCAGAAATTCGTACAACAGCT
CCTGGAGCTTTTTGATAGTGAAGATCCCAGAGAACGTGACTTCCTGAAGACTGTTCTGCA
CCGAATTTATGGGAAATTTCTTGGATTAAGAGCATTCATCAGAAAACAAATTAACAACAT
TTTCCTCAGGTTTATATATGAAACAGAACATTTCAATGGTGTTGCTGAACTTCTTGAAAT
ATTAGGAAGTATTATCAATGGCTTTGCATTGCCACTGAAAGCAGAACATAAACAATTTCT
AATGAAGGTTCTTATTCCTATGCATACTGCAAAAGGATTAGCTTTGTTTCATGCTCAGCT
AGCATATTGTGTTGTACAGTTCCTGGAGAAAGATACAACACTAACAGAGCCAGTGATCAG
AGGACTGCTGAAATTTTGGCCAAAAACCTGCAGTCAGAAAGAGGTGATGTTTTTAGGAGA
AATTGAAGAAATCTTAGATGTCATTGAACCAACACAGTTCAAAAAAATTGAAGAGCCACT
TTTCAAGCAGATATCCAAGTGTGTATCCAGTTCTCATTTTCAGGTTGCAGAAAGGGCATT
GTACTTCTGGAATAACGAATATATTCTTAGTTTGATTGAGGAGAACATTGATAAAATTCT
GCCAATTATGTTTGCCAGTTTGTACAAAATTTCCAAAGAACACTGGAATCCGACCATTGT
AGCACTGGTATACAATGTGCTGAAAACCCTAATGGAAATGAATGGCAAGCTTTTCGATGA
CCTTACTAGCTCATACAAAGCTGAAAGACAGAGAGAGAAAAAGAAGGAATTGGAACGTGA
AGAATTATGGAAAAAATTAGAGGAGCTAAAGCTAAAGAAAGCTCTAGAAAAACAGAATAG
TGCTTACAACATGCACAGTATTCTCAGCAATACAAGTGCCGAAGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT