RIC8A ORF clone FHC02733

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02733

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Clone info.

Species

human

Clone Name

pFN21AB8299 Sequence (with vector) Restriction Map

Product ID

FHC02733

Flexi Clone PID

FXC02733

ORF Sequence

DNA sequence (1611 bp)
Protein sequence (537 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464144]

Gene

KIBB8299

Symbol

RIC8A

Alias : RIC8

Description

RIC8 guanine nucleotide exchange factor A, transcript variant 1

Original Clone ID

oc00075

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1611 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 537 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7690.1 0 100.0 RIC8A CCDS65982.1 0 98.9 RIC8A CCDS81734.1 4.2e-54 47.4 RIC8B CCDS81733.1 1.5e-48 47.7 RIC8B CCDS9109.2 1.5e-36 46.7 RIC8B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR008376 328 341 PR01802 Synembryn IPR008376 367 385 PR01802 Synembryn IPR008376 399 412 PR01802 Synembryn IPR008376 414 430 PR01802 Synembryn IPR008376 445 462 PR01802 Synembryn IPR008376 477 490 PR01802 Synembryn HMMPfam IPR019318 66 504 PF10165 Guanine nucleotide exchange factor
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_068751 537 609146 100.0 100.0 NP_001273063 531 609146 98.9 100.0 XP_005253109 535 609146 98.2 100.0 NP_001317075 544 609147 46.7 99.1 NP_001317074 560 609147 47.4 99.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8299 Download

>KIBB8299  1611 bp
ATGGAGCCCCGGGCGGTTGCAGAAGCCGTGGAGACGGGTGAGGAGGATGTGATTATGGAA
GCTCTGCGGTCATACAACCAGGAGCACTCCCAGAGCTTCACGTTTGATGATGCCCAACAG
GAGGACCGGAAGAGACTGGCGGAGCTGCTGGTCTCCGTCCTGGAACAGGGCTTGCCACCC
TCCCACCGTGTCATCTGGCTGCAGAGTGTCCGAATCCTGTCCCGGGACCGCAACTGCCTG
GACCCGTTCACCAGCCGCCAGAGCCTGCAGGCACTAGCCTGCTATGCTGACATCTCTGTC
TCTGAGGGGTCCGTCCCAGAGTCCGCAGACATGGATGTTGTACTGGAGTCCCTCAAGTGC
CTGTGCAACCTCGTGCTCAGCAGCCCTGTGGCACAGATGCTGGCAGCAGAGGCCCGCCTA
GTGGTGAAGCTCACAGAGCGTGTGGGGCTGTACCGTGAGAGGAGCTTCCCCCACGATGTC
CAGTTCTTTGACTTGCGGCTCCTCTTCCTGCTAACGGCACTCCGCACCGATGTGCGCCAG
CAGCTGTTTCAGGAGCTGAAAGGAGTGCGCCTGCTAACTGACACACTGGAGCTGACGCTG
GGGGTGACTCCTGAAGGGAACCCCCCACCCACGCTCCTTCCTTCCCAAGAGACTGAGCGG
GCCATGGAGATCCTCAAAGTGCTCTTCAACATCACCCTGGACTCCATCAAGGGGGAGGTG
GACGAGGAAGACGCTGCCCTTTACCGACACCTGGGGACCCTTCTCCGGCACTGTGTGATG
ATCGCTACTGCTGGAGACCGCACAGAGGAGTTCCACGGCCACGCAGTGAACCTCCTGGGG
AACTTGCCCCTCAAGTGTCTGGATGTTCTCCTCACCCTGGAGCCACATGGAGACTCCACG
GAGTTCATGGGAGTGAATATGGATGTGATTCGTGCCCTCCTCATCTTCCTAGAGAAGCGT
TTGCACAAGACACACAGGCTGAAGGAGAGTGTAGCTCCCGTGCTGAGCGTGCTGACTGAA
TGTGCCCGGATGCACCGCCCAGCCAGGAAGTTCCTGAAGGCCCAGGGATGGCCACCTCCC
CAGGTGCTGCCCCCTCTGCGGGATGTGAGGACACGGCCTGAGGTTGGGGAGATGCTGCGG
AACAAGCTTGTCCGCCTCATGACACACCTGGACACAGATGTGAAGAGGGTGGCTGCCGAG
TTCTTGTTTGTCCTGTGCTCTGAGAGTGTGCCCCGATTCATCAAGTACACAGGCTATGGG
AATGCTGCTGGCCTTCTGGCTGCCAGGGGCCTCATGGCAGGAGGCCGGCCCGAGGGCCAG
TACTCAGAGGATGAGGACACAGACACAGATGAGTACAAGGAAGCCAAAGCCAGCATAAAC
CCTGTGACCGGGAGGGTGGAGGAGAAGCCGCCTAACCCTATGGAGGGCATGACAGAGGAG
CAGAAGGAGCACGAGGCCATGAAGCTGGTGACCATGTTTGACAAGCTCTCCAGGAACAGA
GTCATCCAGCCAATGGGGATGAGTCCCCGGGGTCATCTTACGTCCCTGCAGGATGCCATG
TGCGAGACTATGGAGCAGCAGCTCTCCTCGGACCCTGACTCGGACCCTGAC

ORF Protein

Cloned ORF protein sequence for pFN21AB8299 Download

>KIBB8299  537 aa
MEPRAVAEAVETGEEDVIMEALRSYNQEHSQSFTFDDAQQEDRKRLAELLVSVLEQGLPP
SHRVIWLQSVRILSRDRNCLDPFTSRQSLQALACYADISVSEGSVPESADMDVVLESLKC
LCNLVLSSPVAQMLAAEARLVVKLTERVGLYRERSFPHDVQFFDLRLLFLLTALRTDVRQ
QLFQELKGVRLLTDTLELTLGVTPEGNPPPTLLPSQETERAMEILKVLFNITLDSIKGEV
DEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLGNLPLKCLDVLLTLEPHGDST
EFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARKFLKAQGWPPP
QVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYG
NAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEE
QKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8299 Download

>pFN21AB8299  6316 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGCCCCGGGCGGTTGCAGAAGCCGTGGAGAC
GGGTGAGGAGGATGTGATTATGGAAGCTCTGCGGTCATACAACCAGGAGCACTCCCAGAG
CTTCACGTTTGATGATGCCCAACAGGAGGACCGGAAGAGACTGGCGGAGCTGCTGGTCTC
CGTCCTGGAACAGGGCTTGCCACCCTCCCACCGTGTCATCTGGCTGCAGAGTGTCCGAAT
CCTGTCCCGGGACCGCAACTGCCTGGACCCGTTCACCAGCCGCCAGAGCCTGCAGGCACT
AGCCTGCTATGCTGACATCTCTGTCTCTGAGGGGTCCGTCCCAGAGTCCGCAGACATGGA
TGTTGTACTGGAGTCCCTCAAGTGCCTGTGCAACCTCGTGCTCAGCAGCCCTGTGGCACA
GATGCTGGCAGCAGAGGCCCGCCTAGTGGTGAAGCTCACAGAGCGTGTGGGGCTGTACCG
TGAGAGGAGCTTCCCCCACGATGTCCAGTTCTTTGACTTGCGGCTCCTCTTCCTGCTAAC
GGCACTCCGCACCGATGTGCGCCAGCAGCTGTTTCAGGAGCTGAAAGGAGTGCGCCTGCT
AACTGACACACTGGAGCTGACGCTGGGGGTGACTCCTGAAGGGAACCCCCCACCCACGCT
CCTTCCTTCCCAAGAGACTGAGCGGGCCATGGAGATCCTCAAAGTGCTCTTCAACATCAC
CCTGGACTCCATCAAGGGGGAGGTGGACGAGGAAGACGCTGCCCTTTACCGACACCTGGG
GACCCTTCTCCGGCACTGTGTGATGATCGCTACTGCTGGAGACCGCACAGAGGAGTTCCA
CGGCCACGCAGTGAACCTCCTGGGGAACTTGCCCCTCAAGTGTCTGGATGTTCTCCTCAC
CCTGGAGCCACATGGAGACTCCACGGAGTTCATGGGAGTGAATATGGATGTGATTCGTGC
CCTCCTCATCTTCCTAGAGAAGCGTTTGCACAAGACACACAGGCTGAAGGAGAGTGTAGC
TCCCGTGCTGAGCGTGCTGACTGAATGTGCCCGGATGCACCGCCCAGCCAGGAAGTTCCT
GAAGGCCCAGGGATGGCCACCTCCCCAGGTGCTGCCCCCTCTGCGGGATGTGAGGACACG
GCCTGAGGTTGGGGAGATGCTGCGGAACAAGCTTGTCCGCCTCATGACACACCTGGACAC
AGATGTGAAGAGGGTGGCTGCCGAGTTCTTGTTTGTCCTGTGCTCTGAGAGTGTGCCCCG
ATTCATCAAGTACACAGGCTATGGGAATGCTGCTGGCCTTCTGGCTGCCAGGGGCCTCAT
GGCAGGAGGCCGGCCCGAGGGCCAGTACTCAGAGGATGAGGACACAGACACAGATGAGTA
CAAGGAAGCCAAAGCCAGCATAAACCCTGTGACCGGGAGGGTGGAGGAGAAGCCGCCTAA
CCCTATGGAGGGCATGACAGAGGAGCAGAAGGAGCACGAGGCCATGAAGCTGGTGACCAT
GTTTGACAAGCTCTCCAGGAACAGAGTCATCCAGCCAATGGGGATGAGTCCCCGGGGTCA
TCTTACGTCCCTGCAGGATGCCATGTGCGAGACTATGGAGCAGCAGCTCTCCTCGGACCC
TGACTCGGACCCTGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT