FHC02448

Gene/Protein Characteristic Table for FHC02448

Species

human

Clone Name

pFN21AB7421 Sequence (with vector) Restriction Map

Product ID

FHC02448

Corresponding
Flexi Clone PID

FXC02448

ORF Sequence

DNA sequence (1107 bp)
Protein sequence (369 aa)

Linker Type

Flexi type

The extra Val codon is attached to the 3'-end of the ORF, because a Pme I site ...

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463961]

Gene

KIBB7421

Symbol

OR2T1

Alias : OR1-25

Description

olfactory receptor, family 2, subfamily T, member 1

Original Clone ID

oc00635

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1107 bp
Restriction map A Restriction map B

Features of the protein sequence

Description

Length: 369 aa
Result of homology search against nr database ( FASTA output, Multiple alignment) Entry Exp ID% Protein Source AAI48404 1.2e-154 100.0 Olfactory recep... synthetic construct EAW77226 3.6e-130 100.0 olfactory recep... Homo sapiens O43869 3.8e-127 100.0 Olfactory recep... Homo sapiens XP_001144276 3.5e-124 99.6 similar to Olfa... Pan troglodytes XP_001085200 2e-121 94.6 similar to olfa... Macaca mulatta The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 76 100 PR00237 Rhodopsin-like GPCR superfamily IPR000276 109 130 PR00237 Rhodopsin-like GPCR superfamily IPR000725 142 153 PR00245 Olfactory receptor IPR000276 154 176 PR00237 Rhodopsin-like GPCR superfamily IPR000725 179 191 PR00245 Olfactory receptor IPR000725 226 242 PR00245 Olfactory receptor IPR000276 249 272 PR00237 Rhodopsin-like GPCR superfamily IPR000725 286 295 PR00245 Olfactory receptor IPR000276 322 348 PR00237 Rhodopsin-like GPCR superfamily IPR000725 333 344 PR00245 Olfactory receptor HMMPfam IPR000276 91 340 PF00001 Rhodopsin-like GPCR superfamily ProfileScan IPR000276 91 340 PS50262 Rhodopsin-like GPCR superfamily ScanRegExp IPR000276 160 176 PS00237 Rhodopsin-like GPCR superfamily
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 4 EYYFLNVFFPLLKVCCLTINSHV 26 PRIMARY 23 2 30 LPWECYHLIWKILPYIGTTVGSM 52 SECONDARY 23 3 76 LIFAIISIIFFTALMANGVMIFL 98 PRIMARY 23 4 111 FLLSHLSLIDMMYISTIVPKMLV 133 SECONDARY 23 5 150 QHFLYLTLVGAEFFLLGLMAYD 171 PRIMARY 22 6 190 VCWMIIAGSWFGGSLDGFLLTPI 212 SECONDARY 23 7 254 VLMLLIPFSVVLASYARILTTVQ 276 PRIMARY 23 8 292 SSHMTVVSLFYGAAMYTYMLPHS 314 SECONDARY 23 9 322 KVLSVFYTILTPMLNPLIYSLR 343 SECONDARY 22
Result of homology search against disease genes in OMIM database

(FASTA output, Multiple alignment)

H_id	H_len (aa)	OMIM_No.	ID (%)	Q_ali/Q_len (%)
NP_036501	317	608497	78.7	81.6
NP_003691	308	608494	76.5	82.9
NP_835462	317	608493	76.8	81.6
NP_009091	312	600578	75.4	82.7
NP_003688	314	608492	75.7	81.6