TMEM177 ORF clone FHC02267

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02267

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Clone info.

Species

human

Clone Name

pFN21AB7123 Sequence (with vector) Restriction Map

Product ID

FHC02267

Flexi Clone PID

FXC02267

ORF Sequence

DNA sequence (933 bp)
Protein sequence (311 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464313]

Gene

KIBB7123

Symbol

TMEM177

Description

transmembrane protein 177, transcript variant 2

Original Clone ID

cp01128

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 933 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 311 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2128.1 2e-125 99.4 TMEM177 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition None - - - - -
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 17 TGLLVGSCAGLFGVPVSYHLFPD 39 SECONDARY 23 2 165 TTAVHALLAPACLAGTWALGVGA 187 PRIMARY 23 3 199 NLRAAFSLVAAVAGFVAYAFSQD 221 PRIMARY 23 4 237 SLSAAYACGGVEFYEKLLSGNLA 259 SECONDARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7123 Download

>KIBB7123  933 bp
ATGGCAGGTCCCCTGTGGCGGACCGCAGCATTTGTGCAGAGACACAGGACAGGCCTCTTG
GTGGGTTCCTGTGCAGGCCTGTTTGGAGTTCCAGTCTCGTACCACCTCTTCCCGGATCCC
GTGGTCCAATGGCTCTACCAGTACTGGCCTCAGGGCCAGCCAGCTCCGCTCCCTCCACAG
CTGCAGAGCCTCTTCCAAGAGGTGCTACAGGACATAGGTGTTCCTTCAGGCCATTGCTAC
AAGCCCTTCACCACCTTCACCTTCCAGCCTGTGAGTGCAGGCTTCCCAAGACTCCCTGCT
GGGGCTGTGGTGGGCATCCCTGCCAGTTTCTTGGGAGACCTAGTGATCAACACTAACCAT
CCCGTGGTCATACATGGGCATACAGTGGACTGGCGGAGCCCAGCAGGCGCCCGGCTGAGA
GCTTCCCTGACCTTGTCCCGTGAAGCCCAGAAGTTCGCCTTGGCCAGGGAAGTGGTGTAC
CTGGAAAGCAGTACCACTGCCGTGCACGCCCTGCTGGCCCCAGCTTGCCTGGCAGGGACC
TGGGCACTGGGCGTGGGTGCCAAGTACACCCTGGGGCTCCATGCAGGCCCCATGAATTTA
CGGGCTGCCTTCAGCTTGGTGGCAGCAGTGGCAGGCTTTGTGGCCTACGCCTTCTCCCAG
GATTCTCTCACTCATGCCGTGGAGTCCTGGCTGGACCGCCGCACGGCCTCCCTCTCTGCA
GCCTATGCCTGTGGTGGAGTGGAGTTCTATGAGAAGCTTCTGTCGGGCAACCTGGCCCTG
CGCAGTCTCTTGGGCAAAGAGGGGGAGAAGCTGTATACACCCAGCGGGAACATCGTCCCC
AGACACTTGTTCCGAATCAAACATTTACCCTACACCACCCGCCGGGACTCTGTGCTGCAG
ATGTGGAGGGGGATGCTCAATCCGGGCCGCTCC

ORF Protein

Cloned ORF protein sequence for pFN21AB7123 Download

>KIBB7123  311 aa
MAGPLWRTAAFVQRHRTGLLVGSCAGLFGVPVSYHLFPDPVVQWLYQYWPQGQPAPLPPQ
LQSLFQEVLQDIGVPSGHCYKPFTTFTFQPVSAGFPRLPAGAVVGIPASFLGDLVINTNH
PVVIHGHTVDWRSPAGARLRASLTLSREAQKFALAREVVYLESSTTAVHALLAPACLAGT
WALGVGAKYTLGLHAGPMNLRAAFSLVAAVAGFVAYAFSQDSLTHAVESWLDRRTASLSA
AYACGGVEFYEKLLSGNLALRSLLGKEGEKLYTPSGNIVPRHLFRIKHLPYTTRRDSVLQ
MWRGMLNPGRS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7123 Download

>pFN21AB7123  5638 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGTCCCCTGTGGCGGACCGCAGCATTTGT
GCAGAGACACAGGACAGGCCTCTTGGTGGGTTCCTGTGCAGGCCTGTTTGGAGTTCCAGT
CTCGTACCACCTCTTCCCGGATCCCGTGGTCCAATGGCTCTACCAGTACTGGCCTCAGGG
CCAGCCAGCTCCGCTCCCTCCACAGCTGCAGAGCCTCTTCCAAGAGGTGCTACAGGACAT
AGGTGTTCCTTCAGGCCATTGCTACAAGCCCTTCACCACCTTCACCTTCCAGCCTGTGAG
TGCAGGCTTCCCAAGACTCCCTGCTGGGGCTGTGGTGGGCATCCCTGCCAGTTTCTTGGG
AGACCTAGTGATCAACACTAACCATCCCGTGGTCATACATGGGCATACAGTGGACTGGCG
GAGCCCAGCAGGCGCCCGGCTGAGAGCTTCCCTGACCTTGTCCCGTGAAGCCCAGAAGTT
CGCCTTGGCCAGGGAAGTGGTGTACCTGGAAAGCAGTACCACTGCCGTGCACGCCCTGCT
GGCCCCAGCTTGCCTGGCAGGGACCTGGGCACTGGGCGTGGGTGCCAAGTACACCCTGGG
GCTCCATGCAGGCCCCATGAATTTACGGGCTGCCTTCAGCTTGGTGGCAGCAGTGGCAGG
CTTTGTGGCCTACGCCTTCTCCCAGGATTCTCTCACTCATGCCGTGGAGTCCTGGCTGGA
CCGCCGCACGGCCTCCCTCTCTGCAGCCTATGCCTGTGGTGGAGTGGAGTTCTATGAGAA
GCTTCTGTCGGGCAACCTGGCCCTGCGCAGTCTCTTGGGCAAAGAGGGGGAGAAGCTGTA
TACACCCAGCGGGAACATCGTCCCCAGACACTTGTTCCGAATCAAACATTTACCCTACAC
CACCCGCCGGGACTCTGTGCTGCAGATGTGGAGGGGGATGCTCAATCCGGGCCGCTCCGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT