NFIX ORF clone FHC01876

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC01876

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Clone info.

Species

human

Clone Name

pFN21AB9449 Sequence (with vector) Restriction Map

Product ID

FHC01876

Flexi Clone PID

FXC01876

ORF Sequence

DNA sequence (1395 bp)
Protein sequence (465 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385580]

Gene

KIBB9449

Symbol

NFIX

Alias : CTF, MRSHSS, NF-I/X, NF1-X, NF1A, SOTOS2

Description

nuclear factor I/X (CCAAT-binding transcription factor)

Original Clone ID

cp00137

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1395 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 465 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45996.1 4.9e-150 99.8 NFIX CCDS59359.1 1.7e-146 99.8 NFIX CCDS53322.1 3.7e-102 70.1 NFIA CCDS615.1 4e-100 70.8 NFIA CCDS44156.1 4.1e-100 70.8 NFIA The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019548 29 71 PF10524 CTF transcription factor/nuclear factor 1 IPR003619 92 194 PF03165 MAD homology 1 IPR000647 238 447 PF00859 CTF transcription factor/nuclear factor 1 HMMSmart IPR003619 92 200 SM00523 MAD homology 1 ProfileScan IPR020604 26 219 PS51080 CTF transcription factor/nuclear factor 1
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002492 441 164005,602535,614753 99.8 94.6 XP_005259976 500 164005,602535,614753 97.5 95.5 NP_001257973 433 164005,602535,614753 99.8 92.7 XP_005259975 502 164005,602535,614753 96.1 93.5 XP_005259977 494 164005,602535,614753 96.0 91.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9449 Download

>KIBB9449  1395 bp
ATGCTCCCGGCTTGCCGCCTGCCGGGCCTCCCCTCGCCGCGGCCGGCCGCCGCGCTCCCG
CCCGGGCGCCCAGCTATGTACTCCCCGTACTGCCTCACCCAGGATGAGTTCCACCCGTTC
ATCGAGGCACTGCTGCCTCACGTCCGCGCTTTCTCCTACACCTGGTTCAACCTGCAGGCG
CGGAAGCGCAAGTACTTCAAGAAGCATGAAAAGCGGATGTCGAAGGACGAGGAGCGGGCG
GTGAAGGACGAGCTGCTGGGCGAGAAGCCCGAGATCAAGCAGAAGTGGGCATCCCGGCTG
CTGGCCAAGCTGCGCAAGGACATCCGGCCCGAGTTCCGCGAGGACTTCGTGCTGACCATC
ACGGGCAAGAAGCCCCCCTGCTGCGTGCTCTCCAACCCCGACCAGAAGGGCAAGATCCGG
CGGATTGACTGCCTGCGCCAGGCTGACAAGGTGTGGCGGCTGGACCTGGTCATGGTGATT
TTGTTTAAGGGGATCCCCCTGGAAAGTACTGATGGGGAGCGGCTCTACAAGTCGCCTCAG
TGCTCGAACCCCGGCCTGTGCGTCCAGCCACATCACATTGGAGTCACAATCAAAGAACTG
GATCTTTATCTGGCTTACTTTGTCCACACTCCGGAATCCGGACAATCAGATAGTTCAAAC
CAGCAAGGAGATGCGGACATCAAACCACTGCCCAACGGGCACTTAAGTTTCCAGGACTGT
TTTGTGACTTCCGGGGTCTGGAATGTGACGGAGCTGGTGAGAGTATCACAGACTCCTGTT
GCAACAGCATCAGGGCCCAACTTCTCCCTGGCGGACCTGGAGAGTCCCAGCTACTACAAC
ATCAACCAGGTGACCCTGGGGCGGCGGTCCATCACCTCCCCTCCTTCCACCAGCACCACC
AAGCGCCCCAAGTCCATCGATGACAGTGAGATGGAGAGCCCTGTTGATGACGTGTTCTAT
CCCGGGACAGGCCGTTCCCCAGCAGCTGGCAGCAGCCAGTCCAGCGGGTGGCCCAACGAT
GTGGATGCAGGCCCGGCTTCTCTAAAGAAGTCAGGAAAGCTGGACTTCTGCAGTGCCCTC
TCCTCTCAGGGCAGCTCCCCGCGCATGGCTTTCACCCACCACCCGCTGCCTGTGCTTGCT
GGAGTCAGACCAGGGAGCCCCCGGGCCACAGCATCAGCCCTGCACTTCCCCTCCACGTCC
ATCATCCAGCAGTCGAGCCCGTATTTCACGCACCCGACCATCCGCTACCACCACCACGGG
CAGGACTCACTGAAGGAGTTTGTGCAGTTTGTGTGCTCGGATGGCTCGGGCCAGGCCACC
GGACAGCATTCGCAACGACAGGCGCCTCCTCTGCCAACCGGTTTGTCAGCATCGGACCCC
GGGACGGCAACTTTC

ORF Protein

Cloned ORF protein sequence for pFN21AB9449 Download

>KIBB9449  465 aa
MLPACRLPGLPSPRPAAALPPGRPAMYSPYCLTQDEFHPFIEALLPHVRAFSYTWFNLQA
RKRKYFKKHEKRMSKDEERAVKDELLGEKPEIKQKWASRLLAKLRKDIRPEFREDFVLTI
TGKKPPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLYKSPQ
CSNPGLCVQPHHIGVTIKELDLYLAYFVHTPESGQSDSSNQQGDADIKPLPNGHLSFQDC
FVTSGVWNVTELVRVSQTPVATASGPNFSLADLESPSYYNINQVTLGRRSITSPPSTSTT
KRPKSIDDSEMESPVDDVFYPGTGRSPAAGSSQSSGWPNDVDAGPASLKKSGKLDFCSAL
SSQGSSPRMAFTHHPLPVLAGVRPGSPRATASALHFPSTSIIQQSSPYFTHPTIRYHHHG
QDSLKEFVQFVCSDGSGQATGQHSQRQAPPLPTGLSASDPGTATF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9449 Download

>pFN21AB9449  6100 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTCCCGGCTTGCCGCCTGCCGGGCCTCCCCTC
GCCGCGGCCGGCCGCCGCGCTCCCGCCCGGGCGCCCAGCTATGTACTCCCCGTACTGCCT
CACCCAGGATGAGTTCCACCCGTTCATCGAGGCACTGCTGCCTCACGTCCGCGCTTTCTC
CTACACCTGGTTCAACCTGCAGGCGCGGAAGCGCAAGTACTTCAAGAAGCATGAAAAGCG
GATGTCGAAGGACGAGGAGCGGGCGGTGAAGGACGAGCTGCTGGGCGAGAAGCCCGAGAT
CAAGCAGAAGTGGGCATCCCGGCTGCTGGCCAAGCTGCGCAAGGACATCCGGCCCGAGTT
CCGCGAGGACTTCGTGCTGACCATCACGGGCAAGAAGCCCCCCTGCTGCGTGCTCTCCAA
CCCCGACCAGAAGGGCAAGATCCGGCGGATTGACTGCCTGCGCCAGGCTGACAAGGTGTG
GCGGCTGGACCTGGTCATGGTGATTTTGTTTAAGGGGATCCCCCTGGAAAGTACTGATGG
GGAGCGGCTCTACAAGTCGCCTCAGTGCTCGAACCCCGGCCTGTGCGTCCAGCCACATCA
CATTGGAGTCACAATCAAAGAACTGGATCTTTATCTGGCTTACTTTGTCCACACTCCGGA
ATCCGGACAATCAGATAGTTCAAACCAGCAAGGAGATGCGGACATCAAACCACTGCCCAA
CGGGCACTTAAGTTTCCAGGACTGTTTTGTGACTTCCGGGGTCTGGAATGTGACGGAGCT
GGTGAGAGTATCACAGACTCCTGTTGCAACAGCATCAGGGCCCAACTTCTCCCTGGCGGA
CCTGGAGAGTCCCAGCTACTACAACATCAACCAGGTGACCCTGGGGCGGCGGTCCATCAC
CTCCCCTCCTTCCACCAGCACCACCAAGCGCCCCAAGTCCATCGATGACAGTGAGATGGA
GAGCCCTGTTGATGACGTGTTCTATCCCGGGACAGGCCGTTCCCCAGCAGCTGGCAGCAG
CCAGTCCAGCGGGTGGCCCAACGATGTGGATGCAGGCCCGGCTTCTCTAAAGAAGTCAGG
AAAGCTGGACTTCTGCAGTGCCCTCTCCTCTCAGGGCAGCTCCCCGCGCATGGCTTTCAC
CCACCACCCGCTGCCTGTGCTTGCTGGAGTCAGACCAGGGAGCCCCCGGGCCACAGCATC
AGCCCTGCACTTCCCCTCCACGTCCATCATCCAGCAGTCGAGCCCGTATTTCACGCACCC
GACCATCCGCTACCACCACCACGGGCAGGACTCACTGAAGGAGTTTGTGCAGTTTGTGTG
CTCGGATGGCTCGGGCCAGGCCACCGGACAGCATTCGCAACGACAGGCGCCTCCTCTGCC
AACCGGTTTGTCAGCATCGGACCCCGGGACGGCAACTTTCGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT