MAGED2 ORF clone FHC01488

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC01488

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Clone info.

Species

human

Clone Name

pFN21AB5184 Sequence (with vector) Restriction Map

Product ID

FHC01488

Flexi Clone PID

FXC01488

Original Clone PID

ORK01488

ORF Sequence

DNA sequence (1818 bp)
Protein sequence (606 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385061]

Gene

KIBB5184

Symbol

MAGED2

Alias : 11B6, BARTS5, BCG-1, BCG1, HCA10, MAGE-D2

Description

melanoma antigen family D2, transcript variant 1

Original Clone ID

bm01318

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1818 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 606 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14362.1 4.7e-162 100.0 MAGED2 CCDS43959.1 1.6e-56 45.8 TRO CCDS59528.1 7.1e-54 62.7 TRO CCDS43958.1 7.3e-50 46.1 TRO CCDS59527.1 2.2e-47 69.0 TRO The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002190 286 456 PF01454 MAGE protein ProfileScan IPR002190 279 478 PS50838 MAGE protein
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055414 606 300470,300971 100.0 100.0 NP_957516 606 300470,300971 100.0 100.0 NP_803182 606 300470,300971 100.0 100.0 XP_016885257 1387 300132 45.8 95.0 XP_011529116 1387 300132 45.8 95.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5184 Download

>KIBB5184  1818 bp
ATGTCTGACACAAGCGAGAGTGGTGCAGGTCTAACTCGCTTCCAGGCTGAAGCTTCAGAA
AAGGACAGTAGCTCGATGATGCAGACTCTGTTGACAGTGACCCAGAATGTGGAGGTCCCA
GAGACACCGAAGGCCTCAAAGGCACTGGAGGTCTCAGAGGATGTGAAGGTCTCAAAAGCC
TCTGGGGTCTCAAAGGCCACAGAGGTCTCAAAGACCCCAGAGGCTCGGGAGGCACCTGCC
ACCCAGGCCTCGTCTACTACTCAGCTGACTGATACCCAGGTTCTGGCAGCTGAAAACAAG
AGTCTAGCAGCTGACACCAAGAAACAGAATGCTGACCCGCAGGCTGTGACAATGCCTGCC
ACTGAGACCAAAAAGGTCAGCCATGTGGCTGATACGAAGGTCAATACAAAGGCTCAGGAG
ACTGAGGCTGCACCCTCTCAGGCCCCAGCAGATGAACCTGAGCCTGAGAGTGCAGCTGCC
CAGTCTCAGGAGAATCAGGATACTCGGCCCAAGGTCAAAGCCAAGAAAGCCCGAAAGGTG
AAGCATCTGGATGGGGAAGAGGATGGCAGCAGTGATCAGAGTCAGGCTTCTGGAACCACA
GGTGGCCGAAGGGTCTCAAAGGCTCTAATGGCCTCAATGGCCCGCAGGGCTTCAAGGGGT
CCCATAGCCTTTTGGGCCCGCAGGGCATCAAGGACTCGGTTGGCTGCTTGGGCCCGGAGA
GCCTTGCTCTCCCTGAGATCACCTAAAGCCCGTAGGGGCAAGGCTCGCCGTAGAGCTGCC
AAGCTCCAGTCATCCCAAGAGCCTGAAGCACCACCACCTCGGGATGTGGCCCTTTTGCAA
GGGAGGGCAAATGATTTGGTGAAGTACCTTTTGGCTAAAGACCAGACGAAGATTCCCATC
AAGCGCTCGGACATGCTGAAGGACATCATCAAAGAATACACTGATGTGTACCCCGAAATC
ATTGAACGAGCAGGCTATTCTTTGGAGAAGGTATTTGGGATTCAATTGAAGGAAATTGAT
AAGAATGACCACTTGTACATTCTTCTCAGCACCTTAGAGCCCACTGATGCAGGCATACTG
GGAACGACTAAGGACTCACCCAAGCTGGGTCTGCTCATGGTGCTTCTTAGCATCATCTTC
ATGAATGGAAATCGGTCCAGTGAGGCTGTCATCTGGGAGGTGCTGCGCAAGTTGGGGCTG
CGCCCTGGGATACATCATTCACTCTTTGGGGACGTGAAGAAGCTCATCACTGATGAGTTT
GTGAAGCAGAAGTACCTGGACTATGCCAGAGTCCCCAATAGCAATCCCCCTGAATATGAG
TTCTTCTGGGGCCTGCGCTCTTACTATGAGACCAGCAAGATGAAAGTCCTCAAGTTTGCC
TGCAAGGTACAAAAGAAGGATCCCAAGGAATGGGCAGCTCAGTACCGAGAGGCGATGGAA
GCAGATTTGAAGGCTGCAGCTGAGGCTGCAGCTGAAGCCAAGGCTAGGGCCGAGATTAGA
GCTCGAATGGGCATTGGGCTCGGCTCGGAGAATGCTGCCGGGCCCTGCAACTGGGACGAA
GCTGATATCGGACCCTGGGCCAAAGCCCGGATCCAGGCGGGAGCAGAAGCTAAAGCCAAA
GCCCAAGAGAGTGGCAGTGCCAGCACTGGTGCCAGTACTAGTACCAATAACAGTGCCAGT
GCCAGTGCCAGCACCAGTGGTGGCTTCAGTGCTGGTGCCAGCCTGACCGCCACTCTCACA
TTTGGGCTCTTCGCTGGCCTTGGTGGAGCTGGTGCCAGCACCAGTGGCAGCTCTGGTGCC
TGTGGTTTCTCCTACAAG

ORF Protein

Cloned ORF protein sequence for pFN21AB5184 Download

>KIBB5184  606 aa
MSDTSESGAGLTRFQAEASEKDSSSMMQTLLTVTQNVEVPETPKASKALEVSEDVKVSKA
SGVSKATEVSKTPEAREAPATQASSTTQLTDTQVLAAENKSLAADTKKQNADPQAVTMPA
TETKKVSHVADTKVNTKAQETEAAPSQAPADEPEPESAAAQSQENQDTRPKVKAKKARKV
KHLDGEEDGSSDQSQASGTTGGRRVSKALMASMARRASRGPIAFWARRASRTRLAAWARR
ALLSLRSPKARRGKARRRAAKLQSSQEPEAPPPRDVALLQGRANDLVKYLLAKDQTKIPI
KRSDMLKDIIKEYTDVYPEIIERAGYSLEKVFGIQLKEIDKNDHLYILLSTLEPTDAGIL
GTTKDSPKLGLLMVLLSIIFMNGNRSSEAVIWEVLRKLGLRPGIHHSLFGDVKKLITDEF
VKQKYLDYARVPNSNPPEYEFFWGLRSYYETSKMKVLKFACKVQKKDPKEWAAQYREAME
ADLKAAAEAAAEAKARAEIRARMGIGLGSENAAGPCNWDEADIGPWAKARIQAGAEAKAK
AQESGSASTGASTSTNNSASASASTSGGFSAGASLTATLTFGLFAGLGGAGASTSGSSGA
CGFSYK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5184 Download

>pFN21AB5184  6523 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCTGACACAAGCGAGAGTGGTGCAGGTCTAAC
TCGCTTCCAGGCTGAAGCTTCAGAAAAGGACAGTAGCTCGATGATGCAGACTCTGTTGAC
AGTGACCCAGAATGTGGAGGTCCCAGAGACACCGAAGGCCTCAAAGGCACTGGAGGTCTC
AGAGGATGTGAAGGTCTCAAAAGCCTCTGGGGTCTCAAAGGCCACAGAGGTCTCAAAGAC
CCCAGAGGCTCGGGAGGCACCTGCCACCCAGGCCTCGTCTACTACTCAGCTGACTGATAC
CCAGGTTCTGGCAGCTGAAAACAAGAGTCTAGCAGCTGACACCAAGAAACAGAATGCTGA
CCCGCAGGCTGTGACAATGCCTGCCACTGAGACCAAAAAGGTCAGCCATGTGGCTGATAC
GAAGGTCAATACAAAGGCTCAGGAGACTGAGGCTGCACCCTCTCAGGCCCCAGCAGATGA
ACCTGAGCCTGAGAGTGCAGCTGCCCAGTCTCAGGAGAATCAGGATACTCGGCCCAAGGT
CAAAGCCAAGAAAGCCCGAAAGGTGAAGCATCTGGATGGGGAAGAGGATGGCAGCAGTGA
TCAGAGTCAGGCTTCTGGAACCACAGGTGGCCGAAGGGTCTCAAAGGCTCTAATGGCCTC
AATGGCCCGCAGGGCTTCAAGGGGTCCCATAGCCTTTTGGGCCCGCAGGGCATCAAGGAC
TCGGTTGGCTGCTTGGGCCCGGAGAGCCTTGCTCTCCCTGAGATCACCTAAAGCCCGTAG
GGGCAAGGCTCGCCGTAGAGCTGCCAAGCTCCAGTCATCCCAAGAGCCTGAAGCACCACC
ACCTCGGGATGTGGCCCTTTTGCAAGGGAGGGCAAATGATTTGGTGAAGTACCTTTTGGC
TAAAGACCAGACGAAGATTCCCATCAAGCGCTCGGACATGCTGAAGGACATCATCAAAGA
ATACACTGATGTGTACCCCGAAATCATTGAACGAGCAGGCTATTCTTTGGAGAAGGTATT
TGGGATTCAATTGAAGGAAATTGATAAGAATGACCACTTGTACATTCTTCTCAGCACCTT
AGAGCCCACTGATGCAGGCATACTGGGAACGACTAAGGACTCACCCAAGCTGGGTCTGCT
CATGGTGCTTCTTAGCATCATCTTCATGAATGGAAATCGGTCCAGTGAGGCTGTCATCTG
GGAGGTGCTGCGCAAGTTGGGGCTGCGCCCTGGGATACATCATTCACTCTTTGGGGACGT
GAAGAAGCTCATCACTGATGAGTTTGTGAAGCAGAAGTACCTGGACTATGCCAGAGTCCC
CAATAGCAATCCCCCTGAATATGAGTTCTTCTGGGGCCTGCGCTCTTACTATGAGACCAG
CAAGATGAAAGTCCTCAAGTTTGCCTGCAAGGTACAAAAGAAGGATCCCAAGGAATGGGC
AGCTCAGTACCGAGAGGCGATGGAAGCAGATTTGAAGGCTGCAGCTGAGGCTGCAGCTGA
AGCCAAGGCTAGGGCCGAGATTAGAGCTCGAATGGGCATTGGGCTCGGCTCGGAGAATGC
TGCCGGGCCCTGCAACTGGGACGAAGCTGATATCGGACCCTGGGCCAAAGCCCGGATCCA
GGCGGGAGCAGAAGCTAAAGCCAAAGCCCAAGAGAGTGGCAGTGCCAGCACTGGTGCCAG
TACTAGTACCAATAACAGTGCCAGTGCCAGTGCCAGCACCAGTGGTGGCTTCAGTGCTGG
TGCCAGCCTGACCGCCACTCTCACATTTGGGCTCTTCGCTGGCCTTGGTGGAGCTGGTGC
CAGCACCAGTGGCAGCTCTGGTGCCTGTGGTTTCTCCTACAAGGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT