PDLIM2 ORF clone FHC01034

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Halotag ORF Clone FHC01034

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Clone info.

Species

human

Clone Name

pFN21ASDF0106 Sequence (with vector) Restriction Map

Product ID

FHC01034

Flexi Clone PID

FXC01034

ORF Sequence

DNA sequence (1098 bp)
Protein sequence (366 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384416]

Gene

FLJ00106

Symbol

PDLIM2

Alias : MYSTIQUE, SLIM

Description

PDZ and LIM domain 2 (mystique), transcript variant 1

Original Clone ID

as00106

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1098 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 366 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34860.1 4.7e-85 100.0 PDLIM2 CCDS6032.2 5.4e-75 100.0 PDLIM2 CCDS34861.1 3.1e-56 93.2 PDLIM2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001478 9 79 PF00595 PDZ/DHR/GLGF IPR001781 286 330 PF00412 Zinc finger HMMSmart IPR001478 11 84 SM00228 PDZ/DHR/GLGF ProfileScan IPR001478 1 84 PS50106 PDZ/DHR/GLGF
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_789847 366 609722 100.0 100.0 NP_067643 602 609722 100.0 89.3 NP_055291 364 605889 31.3 89.3 NP_003678 330 166710,603422 34.3 89.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDF0106 Download

>FLJ00106  1098 bp
ATGGCGTTGACGGTGGATGTGGCCGGGCCAGCGCCCTGGGGCTTCCGTATCACAGGGGGC
AGGGATTTCCACACGCCCATCATGGTGACTAAGGTGGCCGAGCGGGGCAAAGCCAAGGAC
GCTGACCTCCGGCCTGGAGACATAATCGTGGCCATCAACGGGGAAAGCGCGGAGGGCATG
CTGCATGCCGAGGCCCAGAGCAAGATCCGCCAGAGCCCCTCGCCCCTGCGGCTGCAGCTG
GACCGGTCTCAGGCTACGTCTCCAGGGCAGACCAATGGGGACAGCTCCTTGGAAGTGCTG
GCGACTCGCTTCCAGGGCTCCGTGAGGACATACACTGAGAGTCAGTCCTCCTTAAGGTCC
TCCTACTCCAGCCCAACCTCCCTCAGCCCGAGGGCCGGCAGCCCCTTCTCACCACCACCC
TCTAGCAGCTCCCTCACTGGAGAGGCGGCCATCAGCCGCAGCTTCCAGAGTCTGGCATGT
TCCCCGGGCCTCCCCGCTGCTGACCGCCTGTCCTACTCAGGCCGCCCTGGAAGCCGACAG
GCCGGCCTCGGCCGCGCTGGCGACTCGGCGGTGCTGGTGCTGCCGCCTTCCCCGGGCCCT
CGTTCCTCCAGGCCCAGCATGGACTCGGAAGGGGGAAGCCTCCTCCTGGACGAGGACTCG
GAAGTCTTCAAGATGCTGCAGGAAAATCGCGAGGGACGGGCGGCCCCCCGACAGTCCAGC
TCCTTTCGGCTCTTGCAGGAAGCCCTGGAGGCTGAGGAGAGAGGTGGCACGCCAGCCTTC
TTGCCCAGCTCACTGAGCCCCCAGTCCTCCCTGCCCGCCTCCAGGGCCCTGGCCACCCCT
CCCAAGCTCCACACTTGTGAGAAGTGCAGTACCAGCATCGCGAACCAGGCTGTGCGCATC
CAGGAGGGCCGGTACCGCCACCCCGGCTGCTACACCTGTGCCGACTGTGGGCTGAACCTG
AAGATGCGCGGGCACTTCTGGGAGGACGCTTGTGCTATGGAGGGAATGAGATTGTCACTG
GAAGCTTTGGAGGGGATGGTGGAGGGCGCCAAGCGGAGGGACAGGAGGAAGACCAGGAGA
CCCATCCAGCCAAGCTGG

ORF Protein

Cloned ORF protein sequence for pFN21ASDF0106 Download

>FLJ00106  366 aa
MALTVDVAGPAPWGFRITGGRDFHTPIMVTKVAERGKAKDADLRPGDIIVAINGESAEGM
LHAEAQSKIRQSPSPLRLQLDRSQATSPGQTNGDSSLEVLATRFQGSVRTYTESQSSLRS
SYSSPTSLSPRAGSPFSPPPSSSSLTGEAAISRSFQSLACSPGLPAADRLSYSGRPGSRQ
AGLGRAGDSAVLVLPPSPGPRSSRPSMDSEGGSLLLDEDSEVFKMLQENREGRAAPRQSS
SFRLLQEALEAEERGGTPAFLPSSLSPQSSLPASRALATPPKLHTCEKCSTSIANQAVRI
QEGRYRHPGCYTCADCGLNLKMRGHFWEDACAMEGMRLSLEALEGMVEGAKRRDRRKTRR
PIQPSW

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDF0106 Download

>pFN21ASDF0106  5827 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCGTTGACGGTGGA
TGTGGCCGGGCCAGCGCCCTGGGGCTTCCGTATCACAGGGGGCAGGGATTTCCACACGCC
CATCATGGTGACTAAGGTGGCCGAGCGGGGCAAAGCCAAGGACGCTGACCTCCGGCCTGG
AGACATAATCGTGGCCATCAACGGGGAAAGCGCGGAGGGCATGCTGCATGCCGAGGCCCA
GAGCAAGATCCGCCAGAGCCCCTCGCCCCTGCGGCTGCAGCTGGACCGGTCTCAGGCTAC
GTCTCCAGGGCAGACCAATGGGGACAGCTCCTTGGAAGTGCTGGCGACTCGCTTCCAGGG
CTCCGTGAGGACATACACTGAGAGTCAGTCCTCCTTAAGGTCCTCCTACTCCAGCCCAAC
CTCCCTCAGCCCGAGGGCCGGCAGCCCCTTCTCACCACCACCCTCTAGCAGCTCCCTCAC
TGGAGAGGCGGCCATCAGCCGCAGCTTCCAGAGTCTGGCATGTTCCCCGGGCCTCCCCGC
TGCTGACCGCCTGTCCTACTCAGGCCGCCCTGGAAGCCGACAGGCCGGCCTCGGCCGCGC
TGGCGACTCGGCGGTGCTGGTGCTGCCGCCTTCCCCGGGCCCTCGTTCCTCCAGGCCCAG
CATGGACTCGGAAGGGGGAAGCCTCCTCCTGGACGAGGACTCGGAAGTCTTCAAGATGCT
GCAGGAAAATCGCGAGGGACGGGCGGCCCCCCGACAGTCCAGCTCCTTTCGGCTCTTGCA
GGAAGCCCTGGAGGCTGAGGAGAGAGGTGGCACGCCAGCCTTCTTGCCCAGCTCACTGAG
CCCCCAGTCCTCCCTGCCCGCCTCCAGGGCCCTGGCCACCCCTCCCAAGCTCCACACTTG
TGAGAAGTGCAGTACCAGCATCGCGAACCAGGCTGTGCGCATCCAGGAGGGCCGGTACCG
CCACCCCGGCTGCTACACCTGTGCCGACTGTGGGCTGAACCTGAAGATGCGCGGGCACTT
CTGGGAGGACGCTTGTGCTATGGAGGGAATGAGATTGTCACTGGAAGCTTTGGAGGGGAT
GGTGGAGGGCGCCAAGCGGAGGGACAGGAGGAAGACCAGGAGACCCATCCAGCCAAGCTG
GTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT