MARK4 ORF clone FHC00935

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00935

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Clone info.

Species

human

Clone Name

pFN21ASDA1860 Sequence (with vector) Restriction Map

Product ID

FHC00935

Flexi Clone PID

FXC00935

ORF Sequence

DNA sequence (2064 bp)
Protein sequence (688 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384310]

Gene

KIAA1860

Symbol

MARK4

Alias : MARK4L, MARK4S, MARKL1, MARKL1L, PAR-1D

Description

MAP/microtubule affinity-regulating kinase 4, transcript variant 2

Original Clone ID

fh18358

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2064 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 688 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12658.1 1.5e-130 100.0 MARK4 CCDS56097.1 1.2e-118 99.2 MARK4 CCDS45166.1 1.9e-75 63.8 MARK3 CCDS45165.1 5.4e-75 67.7 MARK3 CCDS41993.1 8e-75 64.1 MARK3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017442 59 310 PF00069 Serine/threonine-protein kinase-like domain IPR000449 332 366 PF00627 Ubiquitin-associated/translation elongation factor EF1B HMMSmart IPR002290 59 310 SM00220 Serine/threonine-protein kinase domain IPR020635 59 309 SM00219 Tyrosine-protein kinase IPR015940 331 368 SM00165 Ubiquitin-associated/translation elongation factor EF1B ProfileScan IPR000719 59 310 PS50011 Protein kinase IPR015940 324 368 PS50030 Ubiquitin-associated/translation elongation factor EF1B
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_113605 688 606495 100.0 100.0 NP_001186796 752 606495 99.2 92.0 NP_001122391 744 602678 63.8 97.8 XP_005267698 738 602678 64.2 97.8 NP_001122390 753 602678 67.7 91.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1860 Download

>KIAA1860  2064 bp
ATGTCTTCGCGGACGGTGCTGGCCCCGGGCAACGATCGGAACTCGGACACGCATGGCACC
TTGGGCAGTGGCCGCTCCTCGGACAAAGGCCCGTCCTGGTCCAGCCGCTCACTGGGTGCC
CGTTGCCGGAACTCCATCGCCTCCTGTCCCGAGGAGCAGCCCCACGTGGGCAACTACCGC
CTGCTGAGGACCATTGGGAAGGGCAACTTTGCCAAAGTCAAGCTGGCTCGGCACATCCTC
ACTGGTCGGGAGGTTGCCATCAAGATTATCGACAAAACCCAGCTGAATCCCAGCAGCCTG
CAGAAGCTGTTCCGAGAAGTCCGCATCATGAAGGGCCTAAACCACCCCAACATCGTGAAG
CTCTTTGAGGTGATTGAGACTGAGAAGACGCTGTACCTGGTGATGGAGTACGCAAGTGCT
GGAGAAGTGTTTGACTACCTCGTGTCGCATGGCCGCATGAAGGAGAAGGAAGCTCGAGCC
AAGTTCCGACAGATTGTTTCGGCTGTGCACTATTGTCACCAGAAAAATATTGTACACAGG
GACCTGAAGGCTGAGAACCTCTTGCTGGATGCCGAGGCCAACATCAAGATTGCTGACTTT
GGCTTCAGCAACGAGTTCACGCTGGGATCGAAGCTGGACACGTTCTGCGGGAGCCCCCCA
TATGCCGCCCCGGAGCTGTTTCAGGGCAAGAAGTACGACGGGCCGGAGGTGGACATCTGG
AGCCTGGGAGTCATCCTGTACACCCTCGTCAGCGGCTCCCTGCCCTTCGACGGGCACAAC
CTCAAGGAGCTGCGGGAGCGAGTACTCAGAGGGAAGTACCGGGTCCCTTTCTACATGTCA
ACAGACTGTGAGAGCATCCTGCGGAGATTTTTGGTGCTGAACCCAGCTAAACGCTGTACT
CTCGAGCAAATCATGAAAGACAAATGGATCAACATCGGCTATGAGGGTGAGGAGTTGAAG
CCATACACAGAGCCCGAGGAGGACTTCGGGGACACCAAGAGAATTGAGGTGATGGTGGGT
ATGGGCTACACACGGGAAGAAATCAAAGAGTCCTTGACCAGCCAGAAGTACAACGAAGTG
ACCGCCACCTACCTCCTGCTGGGCAGGAAGACTGAGGAGGGTGGGGACCGGGGCGCCCCA
GGGCTGGCCCTGGCACGGGTGCGGGCGCCCAGCGACACCACCAACGGAACAAGTTCCAGC
AAAGGCACCAGCCACAGCAAAGGGCAGCGGAGTTCCTCTTCCACCTACCACCGCCAGCGC
AGGCATAGCGATTTCTGTGGCCCATCCCCTGCACCCCTGCACCCCAAACGCAGCCCGACG
AGCACGGGGGAGGCGGAGCTGAAGGAGGAGCGGCTGCCAGGCCGGAAGGCGAGCTGCAGC
ACCGCGGGGAGTGGGAGTCGAGGGCTGCCCCCCTCCAGCCCCATGGTCAGCAGCGCCCAC
AACCCCAACAAGGCAGAGATCCCAGAGCGGCGGAAGGACAGCACGAGCACCCCCAACAAC
CTCCCTCCTAGCATGATGACCCGCAGAAACACCTACGTTTGCACAGAACGCCCGGGGGCT
GAGCGCCCGTCACTGTTGCCAAATGGGAAAGAAAACAGCTCAGGCACCCCACGGGTGCCC
CCTGCCTCCCCCTCCAGTCACAGCCTGGCACCCCCATCAGGGGAGCGGAGCCGCCTGGCA
CGCGGTTCCACCATCCGCAGCACCTTCCATGGTGGCCAGGTCCGGGACCGGCGGGCAGGG
GGTGGGGGTGGTGGGGGTGTGCAGAATGGGCCCCCTGCCTCTCCCACACTGGCCCATGAG
GCTGCACCCCTGCCCGCCGGGCGGCCCCGCCCCACCACCAACCTCTTCACCAAGCTGACC
TCCAAACTGACCCGAAGGGTTACCCTCGATCCCTCTAAACGGCAGAACTCTAACCGCTGT
GTTTCGGGCGCCTCTCTGCCCCAGGGATCCAAGATCAGGTCGCAGACGAACCTGAGAGAA
TCGGGGGACCTGAGGTCACAAGTTGCCATCTACCTTGGGATCAAACGGAAACCGCCCCCC
GGCTGCTCCGATTCCCCTGGAGTG

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1860 Download

>KIAA1860  688 aa
MSSRTVLAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYR
LLRTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVK
LFEVIETEKTLYLVMEYASAGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHR
DLKAENLLLDAEANIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDIW
SLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCT
LEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKESLTSQKYNEV
TATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGTSHSKGQRSSSSTYHRQR
RHSDFCGPSPAPLHPKRSPTSTGEAELKEERLPGRKASCSTAGSGSRGLPPSSPMVSSAH
NPNKAEIPERRKDSTSTPNNLPPSMMTRRNTYVCTERPGAERPSLLPNGKENSSGTPRVP
PASPSSHSLAPPSGERSRLARGSTIRSTFHGGQVRDRRAGGGGGGGVQNGPPASPTLAHE
AAPLPAGRPRPTTNLFTKLTSKLTRRVTLDPSKRQNSNRCVSGASLPQGSKIRSQTNLRE
SGDLRSQVAIYLGIKRKPPPGCSDSPGV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1860 Download

>pFN21ASDA1860  6793 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGTCTTCGCGGACGGT
GCTGGCCCCGGGCAACGATCGGAACTCGGACACGCATGGCACCTTGGGCAGTGGCCGCTC
CTCGGACAAAGGCCCGTCCTGGTCCAGCCGCTCACTGGGTGCCCGTTGCCGGAACTCCAT
CGCCTCCTGTCCCGAGGAGCAGCCCCACGTGGGCAACTACCGCCTGCTGAGGACCATTGG
GAAGGGCAACTTTGCCAAAGTCAAGCTGGCTCGGCACATCCTCACTGGTCGGGAGGTTGC
CATCAAGATTATCGACAAAACCCAGCTGAATCCCAGCAGCCTGCAGAAGCTGTTCCGAGA
AGTCCGCATCATGAAGGGCCTAAACCACCCCAACATCGTGAAGCTCTTTGAGGTGATTGA
GACTGAGAAGACGCTGTACCTGGTGATGGAGTACGCAAGTGCTGGAGAAGTGTTTGACTA
CCTCGTGTCGCATGGCCGCATGAAGGAGAAGGAAGCTCGAGCCAAGTTCCGACAGATTGT
TTCGGCTGTGCACTATTGTCACCAGAAAAATATTGTACACAGGGACCTGAAGGCTGAGAA
CCTCTTGCTGGATGCCGAGGCCAACATCAAGATTGCTGACTTTGGCTTCAGCAACGAGTT
CACGCTGGGATCGAAGCTGGACACGTTCTGCGGGAGCCCCCCATATGCCGCCCCGGAGCT
GTTTCAGGGCAAGAAGTACGACGGGCCGGAGGTGGACATCTGGAGCCTGGGAGTCATCCT
GTACACCCTCGTCAGCGGCTCCCTGCCCTTCGACGGGCACAACCTCAAGGAGCTGCGGGA
GCGAGTACTCAGAGGGAAGTACCGGGTCCCTTTCTACATGTCAACAGACTGTGAGAGCAT
CCTGCGGAGATTTTTGGTGCTGAACCCAGCTAAACGCTGTACTCTCGAGCAAATCATGAA
AGACAAATGGATCAACATCGGCTATGAGGGTGAGGAGTTGAAGCCATACACAGAGCCCGA
GGAGGACTTCGGGGACACCAAGAGAATTGAGGTGATGGTGGGTATGGGCTACACACGGGA
AGAAATCAAAGAGTCCTTGACCAGCCAGAAGTACAACGAAGTGACCGCCACCTACCTCCT
GCTGGGCAGGAAGACTGAGGAGGGTGGGGACCGGGGCGCCCCAGGGCTGGCCCTGGCACG
GGTGCGGGCGCCCAGCGACACCACCAACGGAACAAGTTCCAGCAAAGGCACCAGCCACAG
CAAAGGGCAGCGGAGTTCCTCTTCCACCTACCACCGCCAGCGCAGGCATAGCGATTTCTG
TGGCCCATCCCCTGCACCCCTGCACCCCAAACGCAGCCCGACGAGCACGGGGGAGGCGGA
GCTGAAGGAGGAGCGGCTGCCAGGCCGGAAGGCGAGCTGCAGCACCGCGGGGAGTGGGAG
TCGAGGGCTGCCCCCCTCCAGCCCCATGGTCAGCAGCGCCCACAACCCCAACAAGGCAGA
GATCCCAGAGCGGCGGAAGGACAGCACGAGCACCCCCAACAACCTCCCTCCTAGCATGAT
GACCCGCAGAAACACCTACGTTTGCACAGAACGCCCGGGGGCTGAGCGCCCGTCACTGTT
GCCAAATGGGAAAGAAAACAGCTCAGGCACCCCACGGGTGCCCCCTGCCTCCCCCTCCAG
TCACAGCCTGGCACCCCCATCAGGGGAGCGGAGCCGCCTGGCACGCGGTTCCACCATCCG
CAGCACCTTCCATGGTGGCCAGGTCCGGGACCGGCGGGCAGGGGGTGGGGGTGGTGGGGG
TGTGCAGAATGGGCCCCCTGCCTCTCCCACACTGGCCCATGAGGCTGCACCCCTGCCCGC
CGGGCGGCCCCGCCCCACCACCAACCTCTTCACCAAGCTGACCTCCAAACTGACCCGAAG
GGTTACCCTCGATCCCTCTAAACGGCAGAACTCTAACCGCTGTGTTTCGGGCGCCTCTCT
GCCCCAGGGATCCAAGATCAGGTCGCAGACGAACCTGAGAGAATCGGGGGACCTGAGGTC
ACAAGTTGCCATCTACCTTGGGATCAAACGGAAACCGCCCCCCGGCTGCTCCGATTCCCC
TGGAGTGTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT