POFUT2 ORF clone FHC00697

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00697

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Clone info.

Species

human

Clone Name

pFN21ASDA0958 Sequence (with vector) Restriction Map

Product ID

FHC00697

Flexi Clone PID

FXC00697

ORF Sequence

DNA sequence (1272 bp)
Protein sequence (424 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384059]

Gene

KIAA0958

Symbol

POFUT2

Alias : C21orf80, FUT13

Description

protein O-fucosyltransferase 2, transcript variant 1

Original Clone ID

hj05707

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1272 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 424 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13721.1 2.6e-196 100.0 POFUT2 CCDS13719.1 1.6e-174 98.2 POFUT2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019378 46 379 PF10250 GDP-fucose protein O-fucosyltransferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MATLSFVFLLLGAVSWPP 18 PRIMARY 18
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056042 424 610249 100.0 100.0 NP_598368 429 610249 98.2 91.7 XP_006724044 449 610249 93.4 91.7 XP_011527807 488 610249 84.2 81.8 XP_011527808 474 610249 87.7 88.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0958 Download

>KIAA0958  1272 bp
ATGGCGACACTCAGCTTCGTCTTCCTGCTGCTGGGGGCAGTGTCCTGGCCTCCGGCTTCT
GCCTCCGGCCAGGAGTTCTGGCCCGGACAATCGGCGGCCGATATTCTGTCGGGGGCGGCT
TCCCGCAGACGGTATCTTCTGTATGACGTCAACCCCCCGGAAGGCTTCAACCTGCGCAGG
GATGTCTATATCCGAATCGCCTCTCTCCTGAAGACTCTGCTGAAGACGGAGGAGTGGGTG
CTTGTCCTGCCTCCATGGGGCCGCCTCTATCACTGGCAGAGTCCTGACATCCACCAGGTC
CGGATTCCCTGGTCTGAGTTTTTTGATCTTCCAAGTCTCAATAAAAACATCCCCGTCATC
GAGTATGAGCAGTTCATCGCAGAATCTGGTGGGCCCTTTATTGACCAGGTTTACGTCCTG
CAAAGTTACGCAGAGGGGTGGAAAGAAGGGACCTGGGAAGAGAAGGTGGACGAGCGGCCG
TGTATTGATCAGCTCCTGTACTCCCAGGACAAGCACGAGTACTACAGAGGATGGTTTTGG
GGTTATGAGGAGACCAGGGGTCTAAACGTCTCCTGTCTGTCCGTCCAGGGCTCAGCCTCC
ATCGTGGCGCCCCTGCTGCTGAGAAACACATCAGCCCGGTCCGTGATGTTAGACAGAGCC
GAGAACCTACTTCACGACCACTATGGAGGGAAAGAATACTGGGATACCCGTCGCAGCATG
GTGTTTGCCAGGCACCTGCGGGAGGTGGGAGACGAGTTCAGGAGCAGACATCTCAACTCC
ACGGACGACGCAGACAGGATCCCCTTCCAGGAGGACTGGATGAAGATGAAGGTCAAGCTG
GGCTCCGCGCTAGGGGGCCCCTACCTGGGAGTCCACCTGAGAAGAAAAGATTTCATCTGG
GGTCACAGACAGGATGTACCCAGTCTGGAAGGGGCCGTGAGGAAGATCCGCAGCCTCATG
AAGACCCACCGGCTGGACAAGGTGTTTGTGGCCACAGATGCCGTCAGAAAGGAATATGAA
GAGCTAAAAAAGCTGTTACCCGAGATGGTGAGGTTTGAACCCACGTGGGAGGAGCTGGAG
CTCTACAAGGACGGAGGCGTTGCGATTATTGACCAGTGGATCTGCGCACACGCCAGGTGC
CTGCCCACGTCACTGTCGGCCGAGAGCGGGTCGGGTGGCTTTCAAAGGTTCTTCTGTCCC
AAGTACTCGGTGTCAGAGCAGATGGTCGCCTGTGTTCACAGTGGTCATTTCCATACTGTT
TGCCTCCTCGTC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0958 Download

>KIAA0958  424 aa
MATLSFVFLLLGAVSWPPASASGQEFWPGQSAADILSGAASRRRYLLYDVNPPEGFNLRR
DVYIRIASLLKTLLKTEEWVLVLPPWGRLYHWQSPDIHQVRIPWSEFFDLPSLNKNIPVI
EYEQFIAESGGPFIDQVYVLQSYAEGWKEGTWEEKVDERPCIDQLLYSQDKHEYYRGWFW
GYEETRGLNVSCLSVQGSASIVAPLLLRNTSARSVMLDRAENLLHDHYGGKEYWDTRRSM
VFARHLREVGDEFRSRHLNSTDDADRIPFQEDWMKMKVKLGSALGGPYLGVHLRRKDFIW
GHRQDVPSLEGAVRKIRSLMKTHRLDKVFVATDAVRKEYEELKKLLPEMVRFEPTWEELE
LYKDGGVAIIDQWICAHARCLPTSLSAESGSGGFQRFFCPKYSVSEQMVACVHSGHFHTV
CLLV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0958 Download

>pFN21ASDA0958  6001 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCGACACTCAGCTT
CGTCTTCCTGCTGCTGGGGGCAGTGTCCTGGCCTCCGGCTTCTGCCTCCGGCCAGGAGTT
CTGGCCCGGACAATCGGCGGCCGATATTCTGTCGGGGGCGGCTTCCCGCAGACGGTATCT
TCTGTATGACGTCAACCCCCCGGAAGGCTTCAACCTGCGCAGGGATGTCTATATCCGAAT
CGCCTCTCTCCTGAAGACTCTGCTGAAGACGGAGGAGTGGGTGCTTGTCCTGCCTCCATG
GGGCCGCCTCTATCACTGGCAGAGTCCTGACATCCACCAGGTCCGGATTCCCTGGTCTGA
GTTTTTTGATCTTCCAAGTCTCAATAAAAACATCCCCGTCATCGAGTATGAGCAGTTCAT
CGCAGAATCTGGTGGGCCCTTTATTGACCAGGTTTACGTCCTGCAAAGTTACGCAGAGGG
GTGGAAAGAAGGGACCTGGGAAGAGAAGGTGGACGAGCGGCCGTGTATTGATCAGCTCCT
GTACTCCCAGGACAAGCACGAGTACTACAGAGGATGGTTTTGGGGTTATGAGGAGACCAG
GGGTCTAAACGTCTCCTGTCTGTCCGTCCAGGGCTCAGCCTCCATCGTGGCGCCCCTGCT
GCTGAGAAACACATCAGCCCGGTCCGTGATGTTAGACAGAGCCGAGAACCTACTTCACGA
CCACTATGGAGGGAAAGAATACTGGGATACCCGTCGCAGCATGGTGTTTGCCAGGCACCT
GCGGGAGGTGGGAGACGAGTTCAGGAGCAGACATCTCAACTCCACGGACGACGCAGACAG
GATCCCCTTCCAGGAGGACTGGATGAAGATGAAGGTCAAGCTGGGCTCCGCGCTAGGGGG
CCCCTACCTGGGAGTCCACCTGAGAAGAAAAGATTTCATCTGGGGTCACAGACAGGATGT
ACCCAGTCTGGAAGGGGCCGTGAGGAAGATCCGCAGCCTCATGAAGACCCACCGGCTGGA
CAAGGTGTTTGTGGCCACAGATGCCGTCAGAAAGGAATATGAAGAGCTAAAAAAGCTGTT
ACCCGAGATGGTGAGGTTTGAACCCACGTGGGAGGAGCTGGAGCTCTACAAGGACGGAGG
CGTTGCGATTATTGACCAGTGGATCTGCGCACACGCCAGGTGCCTGCCCACGTCACTGTC
GGCCGAGAGCGGGTCGGGTGGCTTTCAAAGGTTCTTCTGTCCCAAGTACTCGGTGTCAGA
GCAGATGGTCGCCTGTGTTCACAGTGGTCATTTCCATACTGTTTGCCTCCTCGTCTACGT
AGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T