AMIGO3 ORF clone FHC00287

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00287

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Clone info.

Species

human

Clone Name

pFN21AA1851 Sequence (with vector) Restriction Map

Product ID

FHC00287

Flexi Clone PID

FXC00287

ORF Sequence

DNA sequence (1512 bp)
Protein sequence (504 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385510]

Gene

KIAA1851

Symbol

AMIGO3

Alias : ALI3, AMIGO-3

Description

adhesion molecule with Ig-like domain 3

Original Clone ID

hg04492

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1512 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 504 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33759.1 2.9e-171 100.0 AMIGO3 CCDS8751.1 6e-39 36.5 AMIGO2 CCDS30795.1 4.9e-28 37.7 AMIGO1 CCDS34969.1 9.7e-17 32.3 LRRC24 CCDS4369.1 1.6e-11 30.6 SLIT3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001611 184 200 PF00560 Leucine-rich repeat HMMSmart IPR003591 59 83 SM00369 Leucine-rich repeat IPR003591 84 107 SM00369 Leucine-rich repeat IPR003591 109 131 SM00369 Leucine-rich repeat IPR003591 132 155 SM00369 Leucine-rich repeat IPR003591 156 176 SM00369 Leucine-rich repeat IPR003591 183 206 SM00369 Leucine-rich repeat IPR003599 285 372 SM00409 Immunoglobulin subtype IPR003598 291 359 SM00408 Immunoglobulin subtype 2 ProfileScan IPR001611 86 107 PS51450 Leucine-rich repeat IPR001611 110 133 PS51450 Leucine-rich repeat IPR001611 134 155 PS51450 Leucine-rich repeat IPR001611 158 178 PS51450 Leucine-rich repeat IPR001611 184 207 PS51450 Leucine-rich repeat IPR007110 277 370 PS50835 Immunoglobulin-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MTWLVLLGTLLCMLRVGLGT 20 PRIMARY 20 2 32 HNCPYKCICAADLLSCTGLGLQD 54 SECONDARY 23 3 384 GFTTLLGCAVGLVLVLLYLFAPP 406 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_942015 504 615691 100.0 100.0 NP_862830 522 615690 36.5 97.6 XP_005268894 522 615690 36.5 97.6 NP_001137140 522 615690 36.5 97.6 XP_011540114 493 615689 37.7 94.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1851 Download

>KIAA1851  1512 bp
ATGACCTGGTTGGTGCTGCTGGGGACACTGCTCTGCATGCTGCGCGTTGGGTTAGGCACC
CCGGACTCCGAGGGTTTCCCGCCCCGTGCGCTCCACAACTGCCCCTACAAATGTATCTGC
GCTGCCGACCTGCTAAGCTGCACTGGCCTAGGGCTGCAGGACGTGCCAGCCGAGTTACCT
GCCGCTACTGCGGACCTCGACCTGAGCCACAACGCGCTCCAGCGCCTGCGCCCCGGCTGG
TTGGCGCCCCTCTTCCAGCTGCGCGCCCTGCACCTAGACCACAACGAACTAGATGCGCTG
GGTCGCGGCGTCTTCGTCAACGCCAGCGGCCTGAGGCTGCTCGATCTATCATCTAACACG
TTGCGGGCGCTTGGCCGCCACGACCTCGACGGGCTGGGGGCGCTGGAGAAGCTGCTTCTG
TTCAATAACCGCTTGGTGCACTTGGACGAGCATGCCTTCCACGGCCTGCGCGCGCTCAGC
CATCTCTACCTGGGCTGCAACGAACTCGCCTCGTTCTCCTTCGACCACCTGCACGGTCTG
AGCGCCACCCACCTGCTTACTCTGGACCTCTCCTCCAACCGGCTGGGACACATCTCCGTA
CCTGAGCTGGCCGCGCTGCCGGCCTTCCTCAAGAACGGCCTCTACTTGCACAACAACCCT
TTGCCTTGCGACTGCCGCCTCTACCACCTGCTACAGCGCTGGCACCAGCGGGGCCTGAGC
GCCGTGCGCGACTTTGCGCGCGAGTACGTATGCTTGGCCTTCAAGGTACCCGCGTCCCGC
GTGCGCTTCTTCCAGCACAGCCGCGTCTTTGAGAACTGCTCGTCGGCCCCAGCTCTTGGC
CTAGAGCGGCCGGAAGAGCACCTGTACGCGCTGGTGGGTCGGTCCCTGAGGCTTTACTGC
AACACCAGCGTCCCGGCCATGCGCATTGCCTGGGTTTCGCCGCAGCAGGAGCTTCTCAGG
GCGCCAGGATCCCGCGATGGCAGCATCGCGGTGCTGGCCGACGGCAGCTTGGCCATAGGC
AACGTACAGGAGCAGCATGCGGGACTCTTCGTGTGCCTGGCCACTGGGCCCCGCCTGCAC
CACAACCAGACGCACGAGTACAACGTGAGCGTGCACTTTCCGCGCCCAGAGCCCGAGGCT
TTCAACACAGGCTTCACCACACTGCTGGGCTGTGCCGTGGGCCTTGTGCTCGTGCTGCTC
TACCTGTTCGCCCCACCCTGCCGCTGCTGCCGCCGTGCCTGCCGCTGCCGCCGCTGGCCC
CAAACACCCAGCCCGCTCCAAGAGCTGAGCGCACAGTCCTCAGTACTCAGCACCACACCG
CCAGACGCACCCAGCCGCAAGGCCAGCGTCCACAAGCACGTAGTCTTTCTGGAGCCAGGC
CGGAGGGGCCTCAATGGCCGCGTGCAGCTGGCAGTAGCTGAGGAATTCGATCTCTACAAC
CCTGGAGGCCTGCAGCTGAAGGCTGGCTCTGAGTCCGCCAGCTCCATAGGCTCCGAGGGT
CCCATGACAACC

ORF Protein

Cloned ORF protein sequence for pFN21AA1851 Download

>KIAA1851  504 aa
MTWLVLLGTLLCMLRVGLGTPDSEGFPPRALHNCPYKCICAADLLSCTGLGLQDVPAELP
AATADLDLSHNALQRLRPGWLAPLFQLRALHLDHNELDALGRGVFVNASGLRLLDLSSNT
LRALGRHDLDGLGALEKLLLFNNRLVHLDEHAFHGLRALSHLYLGCNELASFSFDHLHGL
SATHLLTLDLSSNRLGHISVPELAALPAFLKNGLYLHNNPLPCDCRLYHLLQRWHQRGLS
AVRDFAREYVCLAFKVPASRVRFFQHSRVFENCSSAPALGLERPEEHLYALVGRSLRLYC
NTSVPAMRIAWVSPQQELLRAPGSRDGSIAVLADGSLAIGNVQEQHAGLFVCLATGPRLH
HNQTHEYNVSVHFPRPEPEAFNTGFTTLLGCAVGLVLVLLYLFAPPCRCCRRACRCRRWP
QTPSPLQELSAQSSVLSTTPPDAPSRKASVHKHVVFLEPGRRGLNGRVQLAVAEEFDLYN
PGGLQLKAGSESASSIGSEGPMTT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1851 Download

>pFN21AA1851  6217 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACCTGGTTGGTGCTGCTGGGGACACTGCTCTG
CATGCTGCGCGTTGGGTTAGGCACCCCGGACTCCGAGGGTTTCCCGCCCCGTGCGCTCCA
CAACTGCCCCTACAAATGTATCTGCGCTGCCGACCTGCTAAGCTGCACTGGCCTAGGGCT
GCAGGACGTGCCAGCCGAGTTACCTGCCGCTACTGCGGACCTCGACCTGAGCCACAACGC
GCTCCAGCGCCTGCGCCCCGGCTGGTTGGCGCCCCTCTTCCAGCTGCGCGCCCTGCACCT
AGACCACAACGAACTAGATGCGCTGGGTCGCGGCGTCTTCGTCAACGCCAGCGGCCTGAG
GCTGCTCGATCTATCATCTAACACGTTGCGGGCGCTTGGCCGCCACGACCTCGACGGGCT
GGGGGCGCTGGAGAAGCTGCTTCTGTTCAATAACCGCTTGGTGCACTTGGACGAGCATGC
CTTCCACGGCCTGCGCGCGCTCAGCCATCTCTACCTGGGCTGCAACGAACTCGCCTCGTT
CTCCTTCGACCACCTGCACGGTCTGAGCGCCACCCACCTGCTTACTCTGGACCTCTCCTC
CAACCGGCTGGGACACATCTCCGTACCTGAGCTGGCCGCGCTGCCGGCCTTCCTCAAGAA
CGGCCTCTACTTGCACAACAACCCTTTGCCTTGCGACTGCCGCCTCTACCACCTGCTACA
GCGCTGGCACCAGCGGGGCCTGAGCGCCGTGCGCGACTTTGCGCGCGAGTACGTATGCTT
GGCCTTCAAGGTACCCGCGTCCCGCGTGCGCTTCTTCCAGCACAGCCGCGTCTTTGAGAA
CTGCTCGTCGGCCCCAGCTCTTGGCCTAGAGCGGCCGGAAGAGCACCTGTACGCGCTGGT
GGGTCGGTCCCTGAGGCTTTACTGCAACACCAGCGTCCCGGCCATGCGCATTGCCTGGGT
TTCGCCGCAGCAGGAGCTTCTCAGGGCGCCAGGATCCCGCGATGGCAGCATCGCGGTGCT
GGCCGACGGCAGCTTGGCCATAGGCAACGTACAGGAGCAGCATGCGGGACTCTTCGTGTG
CCTGGCCACTGGGCCCCGCCTGCACCACAACCAGACGCACGAGTACAACGTGAGCGTGCA
CTTTCCGCGCCCAGAGCCCGAGGCTTTCAACACAGGCTTCACCACACTGCTGGGCTGTGC
CGTGGGCCTTGTGCTCGTGCTGCTCTACCTGTTCGCCCCACCCTGCCGCTGCTGCCGCCG
TGCCTGCCGCTGCCGCCGCTGGCCCCAAACACCCAGCCCGCTCCAAGAGCTGAGCGCACA
GTCCTCAGTACTCAGCACCACACCGCCAGACGCACCCAGCCGCAAGGCCAGCGTCCACAA
GCACGTAGTCTTTCTGGAGCCAGGCCGGAGGGGCCTCAATGGCCGCGTGCAGCTGGCAGT
AGCTGAGGAATTCGATCTCTACAACCCTGGAGGCCTGCAGCTGAAGGCTGGCTCTGAGTC
CGCCAGCTCCATAGGCTCCGAGGGTCCCATGACAACCGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT