ENPP4 ORF clone FHC00142

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00142

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Clone info.

Species

human

Clone Name

pFN21AA0879 Sequence (with vector) Restriction Map

Product ID

FHC00142

Flexi Clone PID

FXC00142

ORF Sequence

DNA sequence (1359 bp)
Protein sequence (453 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385393]

Gene

KIAA0879

Symbol

ENPP4

Alias : NPP4

Description

ectonucleotide pyrophosphatase/phosphodiesterase 4 (putative)

Original Clone ID

hk07371

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1359 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 453 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34468.1 7.3e-205 100.0 ENPP4 CCDS4915.1 1.3e-86 51.9 ENPP5 CCDS5150.2 1.3e-35 38.7 ENPP1 CCDS5148.1 1.4e-33 35.8 ENPP3 CCDS11763.1 3.3e-25 31.8 ENPP7 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002591 28 339 PF01663 Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MKLLVILLFSGLITGFRSDSSSS 23 SECONDARY 23 2 404 LPEAIAIVIGSLLVLTMLTCLII 426 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055751 453 617000 100.0 100.0 NP_001277001 477 617001 51.9 86.1 XP_005249317 477 617001 51.9 86.1 NP_067547 477 617001 51.9 86.1 XP_011513088 477 617001 51.9 86.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0879 Download

>KIAA0879  1359 bp
ATGAAGTTATTAGTAATACTTTTGTTTTCTGGACTTATAACTGGTTTTAGAAGTGACTCT
TCCTCTAGTTTGCCACCTAAGTTACTACTAGTATCCTTTGATGGCTTCAGAGCTGATTAT
CTGAAGAACTATGAATTTCCTCATCTCCAGAATTTTATCAAAGAAGGTGTTTTGGTAGAG
CATGTTAAAAATGTTTTTATCACAAAAACATTTCCAAACCACTACAGTATTGTGACAGGC
TTGTATGAAGAAAGCCATGGCATTGTGGCTAATTCCATGTATGATGCAGTCACAAAGAAA
CACTTTTCTGACTCTAATGACAAGGATCCTTTTTGGTGGAATGAGGCAGTACCTATTTGG
GTGACCAATCAGCTTCAGGAAAACAGATCAAGTGCTGCTGCTATGTGGCCTGGTACTGAT
GTACCCATTCACGATACCATCTCTTCCTATTTTATGAATTACAACTCCTCAGTGTCATTT
GAGGAAAGACTAAATAATATTACTATGTGGCTAAACAATTCGAACCCACCAGTCACCTTT
GCAACACTATATTGGGAAGAACCAGATGCAAGTGGCCACAAATACGGACCTGAAGATAAA
GAAAACATGAGCAGAGTGTTGAAAAAAATAGATGATCTTATCGGTGACTTAGTCCAAAGA
CTCAAGATGTTAGGGCTATGGGAAAATCTTAATGTGATCATTACAAGTGATCATGGGATG
ACCCAGTGTTCTCAGGACAGACTGATAAACCTGGATTCCTGCATCGATCATTCATACTAC
ACTCTTATAGATTTGAGCCCAGTTGCTGCAATACTTCCCAAAATAAATAGAACAGAGGTT
TATAACAAACTGAAAAACTGTAGCCCTCATATGAATGTTTATCTCAAAGAAGACATTCCT
AACAGATTTTATTACCAACATAATGATCGAATTCAGCCCATTATTTTGGTTGCCGATGAA
GGCTGGACAATTGTGCTAAATGAATCATCACAAAAATTAGGTGACCATGGTTATGATAAT
TCTTTGCCTAGTATGCATCCATTTCTAGCTGCCCACGGACCTGCATTTCACAAAGGCTAC
AAGCATAGCACAATTAACATTGTGGATATTTATCCAATGATGTGCCACATCCTGGGATTA
AAACCACATCCCAATAATGGGACCTTTGGTCATACTAAGTGCTTGTTAGTTGACCAGTGG
TGCATTAATCTCCCAGAAGCCATCGCGATTGTTATCGGTTCACTCTTGGTGTTAACCATG
CTAACATGCCTCATAATAATCATGCAGAATAGACTTTCTGTACCTCGTCCATTTTCTCGA
CTTCAGCTACAAGAAGATGATGATGATCCTTTAATTGGG

ORF Protein

Cloned ORF protein sequence for pFN21AA0879 Download

>KIAA0879  453 aa
MKLLVILLFSGLITGFRSDSSSSLPPKLLLVSFDGFRADYLKNYEFPHLQNFIKEGVLVE
HVKNVFITKTFPNHYSIVTGLYEESHGIVANSMYDAVTKKHFSDSNDKDPFWWNEAVPIW
VTNQLQENRSSAAAMWPGTDVPIHDTISSYFMNYNSSVSFEERLNNITMWLNNSNPPVTF
ATLYWEEPDASGHKYGPEDKENMSRVLKKIDDLIGDLVQRLKMLGLWENLNVIITSDHGM
TQCSQDRLINLDSCIDHSYYTLIDLSPVAAILPKINRTEVYNKLKNCSPHMNVYLKEDIP
NRFYYQHNDRIQPIILVADEGWTIVLNESSQKLGDHGYDNSLPSMHPFLAAHGPAFHKGY
KHSTINIVDIYPMMCHILGLKPHPNNGTFGHTKCLLVDQWCINLPEAIAIVIGSLLVLTM
LTCLIIIMQNRLSVPRPFSRLQLQEDDDDPLIG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0879 Download

>pFN21AA0879  6064 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAGTTATTAGTAATACTTTTGTTTTCTGGACT
TATAACTGGTTTTAGAAGTGACTCTTCCTCTAGTTTGCCACCTAAGTTACTACTAGTATC
CTTTGATGGCTTCAGAGCTGATTATCTGAAGAACTATGAATTTCCTCATCTCCAGAATTT
TATCAAAGAAGGTGTTTTGGTAGAGCATGTTAAAAATGTTTTTATCACAAAAACATTTCC
AAACCACTACAGTATTGTGACAGGCTTGTATGAAGAAAGCCATGGCATTGTGGCTAATTC
CATGTATGATGCAGTCACAAAGAAACACTTTTCTGACTCTAATGACAAGGATCCTTTTTG
GTGGAATGAGGCAGTACCTATTTGGGTGACCAATCAGCTTCAGGAAAACAGATCAAGTGC
TGCTGCTATGTGGCCTGGTACTGATGTACCCATTCACGATACCATCTCTTCCTATTTTAT
GAATTACAACTCCTCAGTGTCATTTGAGGAAAGACTAAATAATATTACTATGTGGCTAAA
CAATTCGAACCCACCAGTCACCTTTGCAACACTATATTGGGAAGAACCAGATGCAAGTGG
CCACAAATACGGACCTGAAGATAAAGAAAACATGAGCAGAGTGTTGAAAAAAATAGATGA
TCTTATCGGTGACTTAGTCCAAAGACTCAAGATGTTAGGGCTATGGGAAAATCTTAATGT
GATCATTACAAGTGATCATGGGATGACCCAGTGTTCTCAGGACAGACTGATAAACCTGGA
TTCCTGCATCGATCATTCATACTACACTCTTATAGATTTGAGCCCAGTTGCTGCAATACT
TCCCAAAATAAATAGAACAGAGGTTTATAACAAACTGAAAAACTGTAGCCCTCATATGAA
TGTTTATCTCAAAGAAGACATTCCTAACAGATTTTATTACCAACATAATGATCGAATTCA
GCCCATTATTTTGGTTGCCGATGAAGGCTGGACAATTGTGCTAAATGAATCATCACAAAA
ATTAGGTGACCATGGTTATGATAATTCTTTGCCTAGTATGCATCCATTTCTAGCTGCCCA
CGGACCTGCATTTCACAAAGGCTACAAGCATAGCACAATTAACATTGTGGATATTTATCC
AATGATGTGCCACATCCTGGGATTAAAACCACATCCCAATAATGGGACCTTTGGTCATAC
TAAGTGCTTGTTAGTTGACCAGTGGTGCATTAATCTCCCAGAAGCCATCGCGATTGTTAT
CGGTTCACTCTTGGTGTTAACCATGCTAACATGCCTCATAATAATCATGCAGAATAGACT
TTCTGTACCTCGTCCATTTTCTCGACTTCAGCTACAAGAAGATGATGATGATCCTTTAAT
TGGGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT