ITIH5 ORF clone FXC00301

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Flexi ORF Clone FXC00301

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Clone info.

Species

human

Clone Name

pF1KA1953 Sequence (with vector) Restriction Map

Product ID

FXC00301

HaloTag Clone PID

FHC00301

Original Clone PID

ORK00301

ORF Sequence

DNA sequence (2826 bp)
Protein sequence (942 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1KOF Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384652]

Gene

KIAA1953

Symbol

ITIH5

Alias : ITI-HC5, PP14776

Description

inter-alpha-trypsin inhibitor heavy chain family, member 5, transcript variant 1

Original Clone ID

fk00837s1

Features of the cloned ORF sequence

Description

Length: 2826 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 942 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS31140.2 0 99.9 ITIH5 CCDS31141.1 6.6e-96 34.7 ITIH2 CCDS14361.1 2.3e-86 33.3 ITIH6 CCDS2864.1 1.1e-79 31.7 ITIH1 CCDS54596.1 8.6e-76 33.1 ITIH4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013694 48 161 PF08487 Vault protein inter-alpha-trypsin IPR002035 295 456 PF00092 von Willebrand factor IPR010600 715 909 PF06668 Inter-alpha-trypsin inhibitor heavy chain HMMSmart IPR006587 44 161 SM00609 Vault protein inter-alpha-trypsin IPR002035 293 476 SM00327 von Willebrand factor ProfileScan IPR013694 35 161 PS51468 Vault protein inter-alpha-trypsin IPR002035 295 478 PS50234 von Willebrand factor
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MLLLLGLCLGLSLCVGS 17 PRIMARY 17
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_085046 942 609783 99.9 100.0 XP_011518015 967 609783 97.3 100.0 NP_002207 946 146640 34.7 92.4 NP_002206 911 147270 31.7 93.3 NP_001159921 900 143890,600564 33.1 87.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KA1953 Download

>KIAA1953  2826 bp
ATGCTCCTGCTGCTGGGGCTGTGCCTGGGGCTGTCCCTGTGTGTGGGGTCGCAGGAAGAG
GCGCAGAGCTGGGGCCACTCTTCGGAGCAGGATGGACTCAGGGTCCCGAGGCAAGTCAGA
CTGTTGCAGAGGCTGAAAACCAAACCTTTGATGACAGAATTCTCAGTGAAGTCTACCATC
ATTTCCCGTTATGCCTTCACTACGGTTTCCTGCAGAATGCTGAACAGAGCTTCTGAAGAC
CAGGACATTGAGTTCCAGATGCAGATTCCAGCTGCAGCTTTCATCACCAACTTCACTATG
CTTATTGGAGACAAGGTGTATCAGGGCGAAATTACAGAGAGAGAAAAGAAGAGTGGTGAT
AGGGTAAAAGAGAAAAGGAATAAAACCACAGAAGAAAATGGAGAGAAGGGGACTGAAATA
TTCAGAGCTTCTGCAGTGATTCCCAGCAAAGACAAAGCCGCCTTTTTCCTGAGTTATGAG
GAGCTTCTGCAGAGGCGCCTGGGCAAGTACGAGCACAGCATCAGCGTGCGGCCCCAGCAG
CTGTCCGGGAGGCTGAGCGTGGACGTGAATATCCTGGAGAGCGCGGGCATCGCATCCCTG
GAGGTGCTGCCGCTTCACAACAGCAGGCAGAGGGGCAGTGGGCGCGGGGAAGATGATTCT
GGGCCTCCCCCATCTACTGTCATTAACCAAAATGAAACATTTGCCAACATAATTTTTAAA
CCTACTGTAGTACAACAAGCCAGGATTGCCCAGAATGGAATTTTGGGAGACTTTATCATT
AGATATGACGTCAATAGAGAACAGAGCATTGGGGACATCCAGGTTCTAAATGGCTATTTT
GTGCACTACTTTGCTCCTAAAGACCTTCCTCCTTTACCCAAGAATGTGGTATTCGTGCTT
GACAGCAGTGCTTCTATGGTGGGAACCAAACTCCGGCAGACCAAGGATGCCCTCTTCACA
ATTCTCCATGACCTCCGACCCCAGGACCGTTTCAGTATCATTGGATTTTCCAACCGGATC
AAAGTATGGAAGGACCACTTGATATCAGTCACTCCAGACAGCATCAGGGATGGGAAAGTG
TACATTCACCATATGTCACCCACTGGAGGCACAGACATCAACGGGGCCCTGCAGAGGGCC
ATCAGGCTCCTCAACAAGTACGTGGCCCACAGTGGCATTGGAGACCGGAGCGTGTCCCTC
ATCGTCTTCCTGACGGATGGGAAGCCCACGGTCGGGGAGACGCACACCCTCAAGATCCTC
AACAACACCCGAGAGGCCGCCCGAGGCCAAGTCTGCATCTTCACCATTGGCATCGGCAAC
GACGTGGACTTCAGGCTGCTGGAGAAACTGTCGCTGGAGAACTGTGGCCTCACACGGCGC
GTGCACGAGGAGGAGGACGCAGGCTCGCAGCTCATCGGGTTCTACGATGAAATCAGGACC
CCGCTCCTCTCTGACATCCGCATCGATTATCCCCCCAGCTCAGTGGTGCAGGCCACCAAG
ACCCTGTTCCCCAACTACTTCAACGGCTCGGAGATCATCATTGCGGGGAAGCTGGTGGAC
AGGAAGCTGGATCACCTGCACGTGGAGGTCACCGCCAGCAACAGTAAGAAATTCATCATC
CTGAAGACAGATGTGCCTGTGCGGCCTCAGAAGGCAGGGAAAGATGTCACAGGAAGCCCC
AGGCCTGGAGGCGATGGAGAGGGGGACACCAACCACATCGAGCGTCTCTGGAGCTACCTC
ACCACAAAGGAGCTGCTGAGCTCCTGGCTGCAAAGTGACGATGAACCGGAGAAGGAGCGG
CTGCGGCAGCGGGCCCAGGCCCTGGCTGTGAGCTACCGCTTCCTCACTCCCTTCACCTCC
ATGAAGCTGAGGGGGCCGGTCCCACGCATGGATGGCCTGGAGGAGGCCCACGGCATGTCG
GCTGCCATGGGACCCGAACCGGTGGTGCAGAGCGTGCGAGGAGCTGGCACGCAGCCAGGA
CCTTTGCTCAAGAAGCCATACCAGCCAAGAATTAAAATCTCTAAAACATCAGTGGATGGT
GATCCCCACTTTGTTGTGGATTTCCCCCTGAGCAGACTCACCGTGTGCTTCAACATTGAT
GGGCAGCCCGGGGACATCCTCAGGCTGGTCTCTGATCACAGGGACTCTGGTGTCACAGTG
AACGGAGAGTTAATTGGGGCACCCGCCCCTCCAAATGGCCACAAGAAACAGCGCACTTAC
TTGCGCACTATCACCATCCTCATCAACAAGCCAGAGAGATCTTATCTCGAGATCACACCG
AGCAGAGTCATCTTGGATGGTGGGGACAGACTGGTGCTCCCCTGCAACCAGAGTGTGGTG
GTGGGGAGCTGGGGGCTGGAGGTGTCCGTGTCTGCCAACGCCAATGTCACCGTCACCATC
CAGGGCTCCATAGCCTTTGTCATCCTCATCCACCTCTACAAAAAGCCGGCGCCCTTCCAG
CGACACCACCTGGGTTTCTACATTGCCAACAGCGAGGGCCTTTCCAGCAACTGCCACGGA
CTGCTGGGTCAGTTCCTGAATCAGGATGCCAGACTCACAGAAGACCCTGCAGGGCCCAGC
CAGAACCTCACTCACCCTCTGCTCCTTCAGGTGGGAGAGGGGCCTGAGGCCGTCCTAACA
GTGAAAGGCCACCAAGTCCCAGTGGTCTGGAAGCAAAGGAAGATTTACAACGGGGAAGAG
CAGATAGACTGCTGGTTTGCCAGGAACAATGCCGCCAAACTGATTGACGGGGAGTACAAG
GATTACCTGGCATCCCATCCATTTGACACAGGGATGACACTTGGCCAGGGAATGTCCAGG
GAGCTC

ORF Protein

Cloned ORF protein sequence for pF1KA1953 Download

>KIAA1953  942 aa
MLLLLGLCLGLSLCVGSQEEAQSWGHSSEQDGLRVPRQVRLLQRLKTKPLMTEFSVKSTI
ISRYAFTTVSCRMLNRASEDQDIEFQMQIPAAAFITNFTMLIGDKVYQGEITEREKKSGD
RVKEKRNKTTEENGEKGTEIFRASAVIPSKDKAAFFLSYEELLQRRLGKYEHSISVRPQQ
LSGRLSVDVNILESAGIASLEVLPLHNSRQRGSGRGEDDSGPPPSTVINQNETFANIIFK
PTVVQQARIAQNGILGDFIIRYDVNREQSIGDIQVLNGYFVHYFAPKDLPPLPKNVVFVL
DSSASMVGTKLRQTKDALFTILHDLRPQDRFSIIGFSNRIKVWKDHLISVTPDSIRDGKV
YIHHMSPTGGTDINGALQRAIRLLNKYVAHSGIGDRSVSLIVFLTDGKPTVGETHTLKIL
NNTREAARGQVCIFTIGIGNDVDFRLLEKLSLENCGLTRRVHEEEDAGSQLIGFYDEIRT
PLLSDIRIDYPPSSVVQATKTLFPNYFNGSEIIIAGKLVDRKLDHLHVEVTASNSKKFII
LKTDVPVRPQKAGKDVTGSPRPGGDGEGDTNHIERLWSYLTTKELLSSWLQSDDEPEKER
LRQRAQALAVSYRFLTPFTSMKLRGPVPRMDGLEEAHGMSAAMGPEPVVQSVRGAGTQPG
PLLKKPYQPRIKISKTSVDGDPHFVVDFPLSRLTVCFNIDGQPGDILRLVSDHRDSGVTV
NGELIGAPAPPNGHKKQRTYLRTITILINKPERSYLEITPSRVILDGGDRLVLPCNQSVV
VGSWGLEVSVSANANVTVTIQGSIAFVILIHLYKKPAPFQRHHLGFYIANSEGLSSNCHG
LLGQFLNQDARLTEDPAGPSQNLTHPLLLQVGEGPEAVLTVKGHQVPVVWKQRKIYNGEE
QIDCWFARNNAAKLIDGEYKDYLASHPFDTGMTLGQGMSREL

ORF+Vector

Nucleotide Sequence (with vector) for pF1KA1953 Download

>pF1KA1953  5939 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGCTCCTGCTG
CTGGGGCTGTGCCTGGGGCTGTCCCTGTGTGTGGGGTCGCAGGAAGAGGCGCAGAGCTGG
GGCCACTCTTCGGAGCAGGATGGACTCAGGGTCCCGAGGCAAGTCAGACTGTTGCAGAGG
CTGAAAACCAAACCTTTGATGACAGAATTCTCAGTGAAGTCTACCATCATTTCCCGTTAT
GCCTTCACTACGGTTTCCTGCAGAATGCTGAACAGAGCTTCTGAAGACCAGGACATTGAG
TTCCAGATGCAGATTCCAGCTGCAGCTTTCATCACCAACTTCACTATGCTTATTGGAGAC
AAGGTGTATCAGGGCGAAATTACAGAGAGAGAAAAGAAGAGTGGTGATAGGGTAAAAGAG
AAAAGGAATAAAACCACAGAAGAAAATGGAGAGAAGGGGACTGAAATATTCAGAGCTTCT
GCAGTGATTCCCAGCAAAGACAAAGCCGCCTTTTTCCTGAGTTATGAGGAGCTTCTGCAG
AGGCGCCTGGGCAAGTACGAGCACAGCATCAGCGTGCGGCCCCAGCAGCTGTCCGGGAGG
CTGAGCGTGGACGTGAATATCCTGGAGAGCGCGGGCATCGCATCCCTGGAGGTGCTGCCG
CTTCACAACAGCAGGCAGAGGGGCAGTGGGCGCGGGGAAGATGATTCTGGGCCTCCCCCA
TCTACTGTCATTAACCAAAATGAAACATTTGCCAACATAATTTTTAAACCTACTGTAGTA
CAACAAGCCAGGATTGCCCAGAATGGAATTTTGGGAGACTTTATCATTAGATATGACGTC
AATAGAGAACAGAGCATTGGGGACATCCAGGTTCTAAATGGCTATTTTGTGCACTACTTT
GCTCCTAAAGACCTTCCTCCTTTACCCAAGAATGTGGTATTCGTGCTTGACAGCAGTGCT
TCTATGGTGGGAACCAAACTCCGGCAGACCAAGGATGCCCTCTTCACAATTCTCCATGAC
CTCCGACCCCAGGACCGTTTCAGTATCATTGGATTTTCCAACCGGATCAAAGTATGGAAG
GACCACTTGATATCAGTCACTCCAGACAGCATCAGGGATGGGAAAGTGTACATTCACCAT
ATGTCACCCACTGGAGGCACAGACATCAACGGGGCCCTGCAGAGGGCCATCAGGCTCCTC
AACAAGTACGTGGCCCACAGTGGCATTGGAGACCGGAGCGTGTCCCTCATCGTCTTCCTG
ACGGATGGGAAGCCCACGGTCGGGGAGACGCACACCCTCAAGATCCTCAACAACACCCGA
GAGGCCGCCCGAGGCCAAGTCTGCATCTTCACCATTGGCATCGGCAACGACGTGGACTTC
AGGCTGCTGGAGAAACTGTCGCTGGAGAACTGTGGCCTCACACGGCGCGTGCACGAGGAG
GAGGACGCAGGCTCGCAGCTCATCGGGTTCTACGATGAAATCAGGACCCCGCTCCTCTCT
GACATCCGCATCGATTATCCCCCCAGCTCAGTGGTGCAGGCCACCAAGACCCTGTTCCCC
AACTACTTCAACGGCTCGGAGATCATCATTGCGGGGAAGCTGGTGGACAGGAAGCTGGAT
CACCTGCACGTGGAGGTCACCGCCAGCAACAGTAAGAAATTCATCATCCTGAAGACAGAT
GTGCCTGTGCGGCCTCAGAAGGCAGGGAAAGATGTCACAGGAAGCCCCAGGCCTGGAGGC
GATGGAGAGGGGGACACCAACCACATCGAGCGTCTCTGGAGCTACCTCACCACAAAGGAG
CTGCTGAGCTCCTGGCTGCAAAGTGACGATGAACCGGAGAAGGAGCGGCTGCGGCAGCGG
GCCCAGGCCCTGGCTGTGAGCTACCGCTTCCTCACTCCCTTCACCTCCATGAAGCTGAGG
GGGCCGGTCCCACGCATGGATGGCCTGGAGGAGGCCCACGGCATGTCGGCTGCCATGGGA
CCCGAACCGGTGGTGCAGAGCGTGCGAGGAGCTGGCACGCAGCCAGGACCTTTGCTCAAG
AAGCCATACCAGCCAAGAATTAAAATCTCTAAAACATCAGTGGATGGTGATCCCCACTTT
GTTGTGGATTTCCCCCTGAGCAGACTCACCGTGTGCTTCAACATTGATGGGCAGCCCGGG
GACATCCTCAGGCTGGTCTCTGATCACAGGGACTCTGGTGTCACAGTGAACGGAGAGTTA
ATTGGGGCACCCGCCCCTCCAAATGGCCACAAGAAACAGCGCACTTACTTGCGCACTATC
ACCATCCTCATCAACAAGCCAGAGAGATCTTATCTCGAGATCACACCGAGCAGAGTCATC
TTGGATGGTGGGGACAGACTGGTGCTCCCCTGCAACCAGAGTGTGGTGGTGGGGAGCTGG
GGGCTGGAGGTGTCCGTGTCTGCCAACGCCAATGTCACCGTCACCATCCAGGGCTCCATA
GCCTTTGTCATCCTCATCCACCTCTACAAAAAGCCGGCGCCCTTCCAGCGACACCACCTG
GGTTTCTACATTGCCAACAGCGAGGGCCTTTCCAGCAACTGCCACGGACTGCTGGGTCAG
TTCCTGAATCAGGATGCCAGACTCACAGAAGACCCTGCAGGGCCCAGCCAGAACCTCACT
CACCCTCTGCTCCTTCAGGTGGGAGAGGGGCCTGAGGCCGTCCTAACAGTGAAAGGCCAC
CAAGTCCCAGTGGTCTGGAAGCAAAGGAAGATTTACAACGGGGAAGAGCAGATAGACTGC
TGGTTTGCCAGGAACAATGCCGCCAAACTGATTGACGGGGAGTACAAGGATTACCTGGCA
TCCCATCCATTTGACACAGGGATGACACTTGGCCAGGGAATGTCCAGGGAGCTCGTTTAA
ACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGA
TCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATA
ACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGG
AACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAG
CCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCA
GTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCG
CTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTG
CCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTC
TTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGG
TCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAG
AGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTC
CGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTG
AATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGC
GCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTG
CCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCT
GATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCG
AAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGAT
CTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGT
ATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATG
GTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGC
TATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCT
GACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTAT
CGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGA
CGCCCAACCGGACTATAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAA
AATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGG
ATCTTCTTGAAATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACC
GCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAAC
TGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCA
CCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGT
GGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACC
GGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCG
AACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCC
CGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCAC
GAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCT
CTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGC
CAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT
TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATAC
CGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAAT
GGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTAT
TCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCAT
TTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGG
GATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTC
CGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGATTAAATC
AGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCC
ACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTC
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAG
ACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATC
CGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCC
CGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTG
CGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT