HUGE

Gene/Protein Characteristic Table for KIAA2025

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK06615
Accession No. :	AB095945
Description :	Roquin.
HUGO Gene Name :	ring finger and CCCH-type zinc finger domains 1 (RC3H1)
Clone Name :	fh08764 [Vector Info]
Source :	Human fetal brain

Features of the cloned DNA sequence	Description

Length: 5273 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1943 bp

Genome contig ID gi89161185r_172072547
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
TTTCCAGTTTTCTTATACATTTGTCTTCTTTTTTT
Flanking genome sequence
(99993 - 99944)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAAAAAAATATATATATATATATATATATATATATATATGT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 r 172172540 172228674 19 99.6 Perfect prediction

Features of the protein sequence	Description

Length: 1109 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q5TC82	100.0	Roquin; RING fi...	Homo sapiens
XP_001151649	99.8	hypothetical pr...	Pan troglodytes
XP_001102746	99.0	roquin isoform ...	Macaca mulatta
CAH70709	100.0	ring finger and...	Homo sapiens
XP_537186	96.9	similar to roqu...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR000571 390 416 PF00642 Zinc finger

HMMSmart IPR000571 389 416 SM00356 Zinc finger

ScanRegExp IPR001841 9 18 PS00518 Zinc finger

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000571	390	416	PF00642	Zinc finger
HMMSmart	IPR000571	389	416	SM00356	Zinc finger
ScanRegExp	IPR001841	9	18	PS00518	Zinc finger

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

- - - - - - -

Method	No.	N terminal	transmembrane region	C terminal	type	length
-	-	-	-	-	-	-

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CCCTATACCCATTGAGATTCC

Primer_r
: CTGAGGTACGAAGGTCTGATC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 1

Experimental conditions

Panel name	: genbank
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	1943 bp
Genome contig ID	gi89161185r_172072547
PolyA signal sequence (None)	+--------+--------+----*----+---- TTTCCAGTTTTCTTATACATTTGTCTTCTTTTTTT
Flanking genome sequence (99993 - 99944)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAATATATATATATATATATATATATATATATATGT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	r	172172540	172228674	19	99.6	Perfect prediction

Primer_f	: CCCTATACCCATTGAGATTCC
Primer_r	: CTGAGGTACGAAGGTCTGATC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA2025

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information