HUGE

Gene/Protein Characteristic Table for KIAA1535

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK05401
Accession No. :	AB040968
Description :	Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3.
HUGO Gene Name :	hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3)
Clone Name :	fk12792 [Vector Info]
Source :	Human fetal brain
Note :	We replaced fh09002, former representative clones for KIAA1535 with fk12792. (2005/08/06)

Features of the cloned DNA sequence	Description

Length: 3522 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1384 bp

Genome contig ID gi89161185f_153414193
PolyA signal sequence
(TATAAA,-18)
+----*----+----*----+----*----+----
AAACAGCTGGGTTTAAATATAAAATAGACACACTC
Flanking genome sequence
(112071 - 112120)
----+----*----+----*----+----*----+----*----+----*
ATTTTTGGCTCTTGGTTTGTGTGTGGGAACAACATGAGTGGGAAGGAATT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 f 153514193 153526262 8 99.4 Perfect prediction

Features of the protein sequence	Description

Length: 711 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9P1Z3	99.9	Potassium/sodiu...	Homo sapiens
AAO49470	99.6	hyperpolarizati...	Homo sapiens
XP_001115891	98.5	hyperpolarizati...	Macaca mulatta
XP_001498862	97.5	similar to hype...	Equus caballus
XP_588153	96.8	similar to Pota...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB033108	5.8e-05	25.1	KIAA1282

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR003938 25 34 PR01463 EAG/ELK/ERG potassium channel

IPR003938 67 77 PR01463 EAG/ELK/ERG potassium channel

IPR003938 78 87 PR01463 EAG/ELK/ERG potassium channel

IPR003938 197 207 PR01463 EAG/ELK/ERG potassium channel

IPR003938 279 288 PR01463 EAG/ELK/ERG potassium channel

IPR003938 443 451 PR01463 EAG/ELK/ERG potassium channel

HMMPfam IPR013621 1 61 PF08412 Ion transport N-terminal

IPR005821 65 283 PF00520 Ion transport

IPR000595 383 468 PF00027 Cyclic nucleotide-binding

HMMSmart IPR000595 365 478 SM00100 Cyclic nucleotide-binding

ProfileScan IPR000595 365 471 PS50042 Cyclic nucleotide-binding

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR003938	25	34	PR01463	EAG/ELK/ERG potassium channel
IPR003938	67	77	PR01463	EAG/ELK/ERG potassium channel
IPR003938	78	87	PR01463	EAG/ELK/ERG potassium channel
IPR003938	197	207	PR01463	EAG/ELK/ERG potassium channel
IPR003938	279	288	PR01463	EAG/ELK/ERG potassium channel
IPR003938	443	451	PR01463	EAG/ELK/ERG potassium channel
HMMPfam	IPR013621	1	61	PF08412	Ion transport N-terminal
IPR005821	65	283	PF00520	Ion transport
IPR000595	383	468	PF00027	Cyclic nucleotide-binding
HMMSmart	IPR000595	365	478	SM00100	Cyclic nucleotide-binding
ProfileScan	IPR000595	365	471	PS50042	Cyclic nucleotide-binding

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 25 PYSDFRFYWDLIMLLLMVGNLIV 47 SECONDARY 23

2 180 DLASAVVRIFNLIGMMLLLCHWD 202 PRIMARY 23

3 263 WLTMLSMIVGATCYAMFIGHATA 285 SECONDARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	25	PYSDFRFYWDLIMLLLMVGNLIV	47	SECONDARY	23
2	180	DLASAVVRIFNLIGMMLLLCHWD	202	PRIMARY	23
3	263	WLTMLSMIVGATCYAMFIGHATA	285	SECONDARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CTTCTGAGTTTGCTGTTGGTG

Primer_r
: TGGTCCCACAGTCTTTAATGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 1

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CTTCTGAGTTTGCTGTTGGTG
Primer_r	: TGGTCCCACAGTCTTTAATGC
PCR product length	: 171 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	1384 bp
Genome contig ID	gi89161185f_153414193
PolyA signal sequence (TATAAA,-18)	+--------+--------+----*----+---- AAACAGCTGGGTTTAAATATAAAATAGACACACTC
Flanking genome sequence (112071 - 112120)	----+--------+--------+--------+--------+----* ATTTTTGGCTCTTGGTTTGTGTGTGGGAACAACATGAGTGGGAAGGAATT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	f	153514193	153526262	8	99.4	Perfect prediction

Gene/Protein Characteristic Table for KIAA1535

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information