HUGE

Gene/Protein Characteristic Table for KIAA1446

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK04262
Accession No. :	AB040879
Description :	Brain-enriched guanylate kinase-associated protein.
HUGO Gene Name :	brain-enriched guanylate kinase-associated homolog (rat) (BEGAIN)
Clone Name :	fg06734 [Vector Info]
Source :	Human fetal brain

Features of the cloned DNA sequence	Description

Length: 5806 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 816 bp

Genome contig ID gi51511730r_99973243
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
TGTATTTTGCATTCATTAATAAAAGACACCGGTGG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAGAATCTGGGTTGGGCTGTTTCTCCTCCTCCTGCCTTTTGGTCTCGGG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 14 r 100073243 100085979 5 99.0 Perfect prediction

Features of the protein sequence	Description

Length: 645 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9BUH8	1.5e-201	99.7	Brain-enriched ...	Homo sapiens
XP_547981	9.1e-187	90.1	similar to like...	Canis lupus fam...
EAW81707	6.9e-182	100.0	likely ortholog...	Homo sapiens
XP_001109589	1.2e-180	98.3	similar to like...	Macaca mulatta
AAC63268	6.4e-175	87.6	brain-enriched ...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

None - - - - -

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 4 GWVCVSVCVCVCVCVR 19 PRIMARY 16

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	4	GWVCVSVCVCVCVCVR	19	PRIMARY	16

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: GGATTCAGAGCAACTACATGG

Primer_r
: TTGCAGTCCTTCCTATAGAGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 14

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: TTTCTTAACGCACTTGGCCTC
Primer_r	: CCTCTTCGTGGACACAACTTC
PCR product length	: 211 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	816 bp
Genome contig ID	gi51511730r_99973243
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- TGTATTTTGCATTCATTAATAAAAGACACCGGTGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAGAATCTGGGTTGGGCTGTTTCTCCTCCTCCTGCCTTTTGGTCTCGGG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	14	r	100073243	100085979	5	99.0	Perfect prediction

Primer_f	: GGATTCAGAGCAACTACATGG
Primer_r	: TTGCAGTCCTTCCTATAGAGC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1446

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information