HUGE

Gene/Protein Characteristic Table for KIAA1421

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB037842
Description :	Vacuolar protein sorting-associated protein 13C.
HUGO Gene Name :
Clone Name :	hk08067 [Vector Info]
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4392 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
YES YES

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 0 bp

Genome contig ID gi51511731r_59933910
PolyA signal sequence
(ATTAAA,-27)
+----*----+----*----+----*----+----
ATTTTGAAATTAAAGAGGTTGTGGTTACTTTGATG
Flanking genome sequence
(97544 - 97495)
----+----*----+----*----+----*----+----*----+----*
AAAAAATCAGAAAAGAAAGGAAGGCCTTTACATGAGCTAAATGTCCTGCA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 15 r 60031454 60139939 39 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 1463 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_510458	98.5	vacuolar protei...	Pan troglodytes
EAW77600	99.9	vacuolar protei...	Homo sapiens
EAW77604	99.9	vacuolar protei...	Homo sapiens
EAW77603	99.9	vacuolar protei...	Homo sapiens
CAF25188	99.9	VPS13C-2B prote...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB007922	1.4e-06	21.9	KIAA0453

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

Motif_DB interpro_ID From To Entry Definition

None - - - - -

Motif_DB	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

- - - - - - -

Method	No.	N terminal	transmembrane region	C terminal	type	length
-	-	-	-	-	-	-

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: ACCAAGAGTACAAGAGACAGG

Primer_r
: CATCAAAGTAACCACAACCTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 15

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CCGGTTATAGTCATCCCACAG
Primer_r	: ATGCCTGGCTGGATCACTGTC
PCR product length	: 188(2.1k) bp
PCR conditions	: 95 °C15 sec66 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	YES	YES
Warning for coding interruption:	YES	NO

Length of 3'UTR	0 bp
Genome contig ID	gi51511731r_59933910
PolyA signal sequence (ATTAAA,-27)	+--------+--------+----*----+---- ATTTTGAAATTAAAGAGGTTGTGGTTACTTTGATG
Flanking genome sequence (97544 - 97495)	----+--------+--------+--------+--------+----* AAAAAATCAGAAAAGAAAGGAAGGCCTTTACATGAGCTAAATGTCCTGCA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	15	r	60031454	60139939	39	100.0	Perfect prediction

Primer_f	: ACCAAGAGTACAAGAGACAGG
Primer_r	: CATCAAAGTAACCACAACCTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1421

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information