HUGE

Gene/Protein Characteristic Table for KIAA1418

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB037839
Description :	Sphingomyelin phosphodiesterase 4.
HUGO Gene Name :	sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4)
Clone Name :	hj05336 [Vector Info]
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4900 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
NO NO

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 1146 bp

Genome contig ID gi89161199r_130525452
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
GACCATGGATCTGAATAAACATGTCCTTGCTTCTG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGTCTTCTGGCACCTGGGCTCAGTCTCTAGGAGGTTCACCTGTACAGAGT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 2 r 130625452 130656793 20 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 889 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
ACA48221	100.0	SKNY protein [H...	Homo sapiens
EAW55597	99.8	hypothetical pr...	Homo sapiens
Q9NXE4	100.0	Sphingomyelin p...	Homo sapiens
XP_001063553	89.2	similar to CG69...	Rattus norvegicus
XP_001058775	89.1	similar to CG69...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

None - - - - -

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 851 VASLFCVGPLPCTLLLTLGYVLY 873 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	851	VASLFCVGPLPCTLLLTLGYVLY	873	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: ATTCACCCCACAGGCGACATG

Primer_r
: GTTTATTCAGATCCATGGTCG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 2

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	NO	NO
Warning for coding interruption:	YES	NO

Length of 3'UTR	1146 bp
Genome contig ID	gi89161199r_130525452
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- GACCATGGATCTGAATAAACATGTCCTTGCTTCTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGTCTTCTGGCACCTGGGCTCAGTCTCTAGGAGGTTCACCTGTACAGAGT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	2	r	130625452	130656793	20	100.0	Perfect prediction

Primer_f	: ATTCACCCCACAGGCGACATG
Primer_r	: GTTTATTCAGATCCATGGTCG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1418

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information