HUGE

Gene/Protein Characteristic Table for KIAA1228

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK04976
Accession No. :	AB033054
Description :	Protein FAM62B.
HUGO Gene Name :	family with sequence similarity 62 (C2 domain containing) member B (FAM62B)
Clone Name :	fh05620s1 [Vector Info]
Source :	Human fetal brain
Note :	We replaced fh05620, former representative clones for KIAA1228 with fh05620s1. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 5742 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 3208 bp

Genome contig ID gi89161213r_158116450
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
CATGTATTTATTCAATAAAACCTTTATGTTAGCTC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGTCGTGATTTGTTTTTCTCTGTCTTTTGAAGGGAGGTGTTCAGTGTGCA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 7 r 158216450 158314949 23 99.3 Perfect prediction

Features of the protein sequence	Description

Length: 843 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
A0FGR8	100.0	Extended synapt...	Homo sapiens
EAX04591	100.0	family with seq...	Homo sapiens
EAX04590	99.9	family with seq...	Homo sapiens
XP_001083514	98.1	similar to fami...	Macaca mulatta
EDL33467	91.9	DNA segment, Ch...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018290	1.3e-78	37.1	KIAA0747
AB023202	5.7e-05	30.3	KIAA0985
AB046817	6.1e-05	32.6	KIAA1597
AB018294	0.0005	23.1	KIAA0751

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR000008 330 342 PR00360 C2 calcium-dependent membrane targeting

IPR000008 354 367 PR00360 C2 calcium-dependent membrane targeting

IPR000008 376 384 PR00360 C2 calcium-dependent membrane targeting

HMMPfam IPR000008 309 395 PF00168 C2 calcium-dependent membrane targeting

IPR000008 460 539 PF00168 C2 calcium-dependent membrane targeting

IPR000008 725 814 PF00168 C2 calcium-dependent membrane targeting

HMMSmart IPR000008 308 410 SM00239 C2 calcium-dependent membrane targeting

IPR000008 459 554 SM00239 C2 calcium-dependent membrane targeting

IPR000008 724 829 SM00239 C2 calcium-dependent membrane targeting

ProfileScan IPR000008 309 395 PS50004 C2 calcium-dependent membrane targeting

IPR000008 445 539 PS50004 C2 calcium-dependent membrane targeting

IPR000008 723 814 PS50004 C2 calcium-dependent membrane targeting

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000008	330	342	PR00360	C2 calcium-dependent membrane targeting
IPR000008	354	367	PR00360	C2 calcium-dependent membrane targeting
IPR000008	376	384	PR00360	C2 calcium-dependent membrane targeting
HMMPfam	IPR000008	309	395	PF00168	C2 calcium-dependent membrane targeting
IPR000008	460	539	PF00168	C2 calcium-dependent membrane targeting
IPR000008	725	814	PF00168	C2 calcium-dependent membrane targeting
HMMSmart	IPR000008	308	410	SM00239	C2 calcium-dependent membrane targeting
IPR000008	459	554	SM00239	C2 calcium-dependent membrane targeting
IPR000008	724	829	SM00239	C2 calcium-dependent membrane targeting
ProfileScan	IPR000008	309	395	PS50004	C2 calcium-dependent membrane targeting
IPR000008	445	539	PS50004	C2 calcium-dependent membrane targeting
IPR000008	723	814	PS50004	C2 calcium-dependent membrane targeting

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 24 GGVLSVELPGLLAQLARSFALLL 46 SECONDARY 23

2 50 ALGYLGLSFSWVLLALALLAWC 71 PRIMARY 22

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	24	GGVLSVELPGLLAQLARSFALLL	46	SECONDARY	23
2	50	ALGYLGLSFSWVLLALALLAWC	71	PRIMARY	22

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: ATCTATAAAGCAGCAGCACTC

Primer_r
: ATTGTGTCTACTCTGCCTAAG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 7

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	3208 bp
Genome contig ID	gi89161213r_158116450
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- CATGTATTTATTCAATAAAACCTTTATGTTAGCTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGTCGTGATTTGTTTTTCTCTGTCTTTTGAAGGGAGGTGTTCAGTGTGCA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	7	r	158216450	158314949	23	99.3	Perfect prediction

Primer_f	: ATCTATAAAGCAGCAGCACTC
Primer_r	: ATTGTGTCTACTCTGCCTAAG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1228

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information