FLJ00270

Product ID	ORK05340
Accession No	AK160368
Clone name	sj07613
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4768 bp) Predicted protein sequence (352 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 352 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD18711	2.2e-146	100.0	FLJ00270 protei...	Homo sapiens
Q8IZF4	7.7e-144	100.0	Probable G-prot...	Homo sapiens
BAF85472	9.9e-143	99.4	unnamed protein...	Homo sapiens
AAN46670	1.6e-128	99.7	G-protein coupl...	Homo sapiens
BAD18748	6.3e-119	100.0	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018311	1.4e-21	32.4	KIAA0768
D87469	2e-19	30.0	KIAA0279
AB018329	2.1e-17	28.4	KIAA0786
AB020628	2.6e-17	28.6	KIAA0821
AB011536	3.1e-15	25.9	KIAA0812

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000203	9	62	PF01825	GPS domain
HMMPfam	IPR000832	69	336	PF00002	G-protein coupled receptor family 2 (secretin-like)
HMMSmart	IPR000203	9	62	SM00303	GPS domain
ProfileScan	IPR000203	10	62	PS50221	GPS domain
ProfileScan	IPR000832	72	331	PS50261	G-protein coupled receptor family 2 (secretin-like)
ScanRegExp	IPR000169	234	244	PS00639	Peptidase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	49	HLTYFAVLMQLSPALVPAELLAP	71	SECONDARY	23
	2	76	SLVGCSISIVASLITVLLHFHF	97	PRIMARY	22
	3	107	IHMNLHASVLLLNIAFLLSPAFA	129	SECONDARY	23
	4	144	ALHYALLSCLTWMAIEGFNLYLL	166	PRIMARY	23
	5	180	FKLGVLGWGAPALLVLLSLSVKS	202	PRIMARY	23
	6	235	VHSVLVMGYGGLTSLFNLVVLAW	257	PRIMARY	23
	7	281	VTVLGLTVLLGTTWALAFFSFGV	303	PRIMARY	23
	8	308	QLFLFTILNSLYGFFLFLWFCSQ	330	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test