Gene/Protein Characteristic Table for KIAA0638
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Product ID ORK00584
Accession No AB014538
Description pleckstrin homology-like domain, family B, member 1, transcript variant 1
Clone name hj03347s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5449 bp)
Predicted protein sequence (1384 aa)
Flexi ORF Clone FXC00584
Source Human adult brain
Rouge ID mKIAA0638 by Kazusa Mouse cDNA Project
Note We replaced hj03347, former representative clones for KIAA0638 with hj03347s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 5449 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1208 bp
Genome contig ID gi51511727f_117883551
PolyA signal sequence
(AATAAA,-26)
+----*----+----*----+----*----+----
CAGCAGAACAATAAAGCCTTTGGACTACGGAAGTG
Flanking genome sequence
(150402 - 150451)
----+----*----+----*----+----*----+----*----+----*
AGTGGAAGGCCGGTGTGGGGCTTGGCGCTGAGGCACTTGGGGATAGGTCT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 11 f 117983547 118033951 23 99.7 Terminal No-hit
Features of the protein sequence
Description

Length: 1384 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q86UU1 0 100.0 Pleckstrin homo...
Homo sapiens
XP_001162204 0 99.7 pleckstrin homo...
Pan troglodytes
EAW67404 0 99.3 pleckstrin homo...
Homo sapiens
XP_546499 0 95.7 similar to plec...
Canis lupus fam...
XP_001162611 0 98.9 pleckstrin homo...
Pan troglodytes
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000253 71 132 PF00498 Forkhead-associated
IPR001849 1264 1377 PF00169 Pleckstrin-like
HMMSmart IPR001849 1264 1379 SM00233 Pleckstrin-like
ProfileScan IPR001849 1263 1377 PS50003 Pleckstrin-like
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f TTAGAGCCAGAAGGGATGAAG
Primer_r ACAGAACTCCAACCATAACCC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 11
Experimental conditions
Panel name GeneBridge 4
Primer_f TTAGAGCCAGAAGGGATGAAG
Primer_r ACAGAACTCCAACCATAACCC
PCR product length 97 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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